2016
DOI: 10.5935/0103-5053.20160040
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The Role of the Conformational Dynamics of Glutathione S-Transferase Epsilon Class on Insecticide Resistance inAnopheles gambiae

Abstract: Glutathione S-transferases (GSTs) are enzymes capable of metabolizing cytotoxic compounds. The enzyme AgGSTE2, member of epsilon class GSTs (GSTE), is the most important GST conferring resistance to dichloro-diphenyl-trichloroethane (DDT) in Anopheles gambiae. We have investigated the conformational dynamics of three GSTE variants (GSTE2, GSTE2-I114T/F120L, GSTE5) from A. gambiae. Large-scale motions of helices H2 and H4 and conformational transition of the C-terminal governs the opening of the G-site and is e… Show more

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Cited by 5 publications
(4 citation statements)
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“…gambiae from West Africa. Pontes et al [50] have demonstrated, in a molecular dynamics study, that AgGSTE2-F120L may be associated with DDT resistance in An. gambiae.…”
Section: Discussionmentioning
confidence: 99%
“…gambiae from West Africa. Pontes et al [50] have demonstrated, in a molecular dynamics study, that AgGSTE2-F120L may be associated with DDT resistance in An. gambiae.…”
Section: Discussionmentioning
confidence: 99%
“…On the other hand, the conformational dynamics of the GSTE class on insecticide resistance in An. gambiae showed noticeable rearrangement for AgGSTE2-F120L and confer increased DDT resistance (Pontes et al., 2016). However, it could be postulated that conformational dynamics study will be helpful in the understanding of the potential impact of D148N mutation on the function of GSTe2.…”
Section: Discussionmentioning
confidence: 99%
“…This mutation modified GSTE2-DDT binding cavity, increasing accessibility of DDT by GSTE2 and consequently increased detoxification of DDT to DDE, thereby resulting in resistance to DDT [93]. In a study by Pontes et al [94], I114T/F120L mutation in GSTE2 of An. gambiae was observed to cause structural rearrangement with a displacement of a Glu116 residue.…”
Section: Metabolic Resistancementioning
confidence: 99%
“…gambiae was observed to cause structural rearrangement with a displacement of a Glu116 residue. The displaced Glu116 was postulated to act as a base that activated GSH, which bound DDT, leading to DDT resistance in the mosquito [94]. Increased expression of GSTE4 is associated with pyrethroid resistance in An.…”
Section: Metabolic Resistancementioning
confidence: 99%