Coupling DLLME-CE for the Stereoselective Analysis of Venlafaxine and Its Main Metabolites after Biotransformation by Fungi

Bortoleto, Marcela Armelim; Bocato, Mariana Zuccherato; Pupo, Mônica Tallarico; Gaitani, Cristiane Masetto de; Oliveira, Anderson Rodrigo Moraes de

doi:10.5935/0103-5053.20150174

Cited by 2 publications

(3 citation statements)

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“…The combination of partial filling capillary electrophoresiselectrospray ionization-mass spectrometry (CE-ESI-MS) was used for the enantioseparation of several basic analytes and their metabolites including VFX and its metabolites. By using a 40 mM ammonium acetate BGE at pH 4.0 containing 2 mg/mL CM-β-CD, good enantioseparation was observed for VFX, ODVFX, and NDVFX (Cherkaoui, Rudaz, Varesio, & Veuthey, 2001 Cunninghamella elegans ATCC 10028B (Bortoleto, Bocato, Pupo, Gaitani, & Oliveira, 2015).…”

Section: Chromatographic Methodsmentioning

confidence: 99%

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Enantioselective analysis of venlafaxine and its active metabolites: A review on the separation methodologies

Hancu

Lupu

Milan

et al. 2020

Biomedical Chromatography

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Venlafaxine (VFX) is a serotonin and norepinephrine reuptake inhibitor chiral drug used in therapy as an antidepressant in the form of a racemate consisting of R-and S-VFX. The two enantiomers of VFX exhibit different pharmacological activities: R-VFX inhibits both norepinephrine and serotonin synaptic reuptake, whereas S-VFX inhibits only the serotonin one. R-and S-VFX are metabolized in the liver to the respective Rand SO -desmethylvenlafaxine (ODVFX), R-and S-N-desmethylvenlafaxine (NDVFX), and R-and S-N,O-didesmethylvenlafaxine (NODVFX). The pharmacological profile of ODVFX is close to that of VFX, whereas the other two chiral metabolites (NDVFX and NODVFX) have lower affinity for the receptor sites. The pharmacokinetics of the VFX enantiomers appear stereoselective, including the metabolism process. In the past 20 years, several studies describing the enantioselective analysis of R-and S-VFX in pharmaceutical formulations and its chiral metabolites in biological matrices were published. These methods encompass liquid chromatography coupled with UV detection, mass spectrometry, or tandem mass spectrometry, and capillary electrophoresis. This paper reviews the published methods used for the determination of the individual enantiomers of VFX and its chiral metabolites in different matrices.

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Section: Chromatographic Methodsmentioning

confidence: 99%

“…The chiral separation was achieved using a 50 mM phosphate BGE at pH 2.0 and a mixture of 1% (m/v) CM‐β‐CD and 8 mM α‐CD as chiral selectors. The study showed a stereoselective biotransformation of VFX into NDVFX by the fungus Cunninghamella elegans ATCC 10028B (Bortoleto, Bocato, Pupo, Gaitani, & Oliveira, 2015).…”

Section: Introductionmentioning

confidence: 99%

Enantioselective analysis of venlafaxine and its active metabolites: A review on the separation methodologies

Hancu

Lupu

Milan

et al. 2020

Biomedical Chromatography

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“…The study demonstrated a stereoselective biotransformation of VFX into S-NDVFX by the fungus Cunninghamella elegans ATCC 10028B with 100% enantiomeric excess (Bortoleto, Bocato, Pupo, Gaitani, & Oliveira, 2015). The optimal electrophoretic conditions for the enantioselective determination methods of VFX are summarized in Table 4.…”

Section: Venlafaxinementioning

confidence: 99%

Capillary electrophoresis in the enantioseparation of modern antidepressants: An overview

Hancu

Budău

Muntean

et al. 2018

Biomedical Chromatography

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Chirality is a key subject in modern drug research as well as in the pharmaceutical industry and drug development. Almost all second-generation modern antidepressants are chiral substances; however in therapy some are used as racemic mixtures while others are used as pure enantiomers. The development of enantioseparation methods of chiral antidepressants and their metabolites is one of the keys in understanding their enantioselective drug action. For this purpose, efficient and reliable analytical methods are needed, and capillary electrophoresis has proved to be an interesting and advantageous alternative to the more frequently used chromatographic techniques. In this review electrodriven methods available for the chiral discrimination of selective serotonin reuptake inhibitors (fluoxetine, citalopram, sertraline) and selective serotonin and norepinephrine reuptake inhibitors (venlafaxine, duloxetine) are presented and discussed.

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Coupling DLLME-CE for the Stereoselective Analysis of Venlafaxine and Its Main Metabolites after Biotransformation by Fungi

Cited by 2 publications

References 1 publication

Enantioselective analysis of venlafaxine and its active metabolites: A review on the separation methodologies

Enantioselective analysis of venlafaxine and its active metabolites: A review on the separation methodologies

Capillary electrophoresis in the enantioseparation of modern antidepressants: An overview

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