Urinary Excretion Profile of Luteinizing Hormone in Brazilian Athletes

Abstract

Luteinizing hormone (LH) can be used by athletes as an alternative way to take illicit advantage of androgenic effects, since it stimulates the secretion of testosterone by the Leydig cells. As consequence, LH use is illegal for male athletes and the hormone is included in the World Anti-Doping Agency (WADA) prohibited list. Since LH is an endogenous substance, the strategy used to allow the detection of LH abuse was the establishment of a reference value. All analyses were developed by IMMULITE ® , which was submitted to a complete validation performance with urine. The hormone showed to be stable at 4 °C and -20 °C for at least 40 days and for 10 days at room temperature and unstable after the second cycle of freezing and thawing. The reference population of male Brazilian athletes showed a cut-off value of 37.4 mIU mL-1. The results indicate the assay is suitable for application in doping control analysis. ]]>

Doping control analysis at the Rio 2016 Olympic and Paralympic Games

“…Added 100 μL MSTFA/NH 4 I/2-mercaptoethanol (1000:2:6), incubated at 60°C for 20 minutes and analysed by GC-MS-MS. [3] hCG/LH Homogenised urine (400 μL) was loaded into the automated immunoassay analyser. [3,7] Anabolic agents, peptide hormones, growth factors, related substances and mimetics, β2 agonists, hormone and metabolic modulators, diuretics and masking agents, stimulants, narcotics, cannabinoids, glucocorticoids, β-blocker Two urine aliquots were used. Aliquot I: 10 μL of urine was diluted in 50 μL of 2% acetic acid, centrifuged (10 G/5 minutes) and stored (10°C).…”

“…The XXXI Summer Olympic Games (August [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]2016) and the XV Paralympic Games (September 7-18, 2016) were held in Rio de Janeiro, marking the first time that these events were organised in South America. Doping control analyses have been conducted in Brazil since 1989, were incorporated into the International Olympic Committee (IOC) accreditation in 2002, and were absorbed by the World Anti-Doping Agency (WADA) accreditation system in 2005.…”