Can Ergosterol Be an Indicator of<i>Fusarium</i>Fungi and Mycotoxins in Cereal Products?

Stanisz, Ewa; Zgoła‐Grześkowiak, Agnieszka; Waśkiewicz, Agnieszka; Stępień, Łukasz; Beszterda, Monika

doi:10.5935/0103-5053.20150030

Cited by 11 publications

(19 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ergosterol was determined according to the procedure described previously . Briefly, 200 mg of sample was placed in a 12 mL glass culture tube, then 2 mL of methanol was added, followed by 0.5 mL of 2 mol L −1 NaOH.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

“…revealed that HPTLC measurement of ergosterol on long‐grain white rice can be a useful method to detect fungal activity and ochratoxin production. However, another study showed a lack of correlation between ergosterol content and mycotoxin production in cereal products …”

Section: Introductionmentioning

confidence: 99%

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ergosterol content is an indicator of the total content of the fungal biomass, including toxigenic and non-toxigenic fungi. This is why high ERG levels do not necessarily correlate with mycotoxin levels, especially in plant samples naturally contaminated with mycotoxins (49). The correlation between ERG and mycotoxins levels, however, was reported in controlled experiments with inoculation with Fusarium pathogens (50).…”

Section: Fusarium Spp and Fusariotoxinsmentioning

confidence: 97%

The effect of fertiliser treatments on the severity of Fusarium head blight and mycotoxin biosynthesis in winter rye

Cwalina-Ambroziak

Kurowski²,

Waśkiewicz

et al. 2017

Archives of Industrial Hygiene and Toxicology

Self Cite

View full text Add to dashboard Cite

The fungi of the genus Fusarium cause Fusarium head blight (FHB), a devastating disease that reduces grain yield and quality. They also produce mycotoxins which may pose a serious threat to human and animal health. This study investigated the effects of NPK fertilisation, foliar application of Cu, Zn, and Mn, applied separately and in combination, and of the Nano-Gro ® organic growth stimulator on the occurrence of FHB in cultivar Dańkowskie Diament rye based on the mycological analysis of kernels and on the concentrations of Fusarium mycotoxins in grain. The severity of FHB caused by seven species of the genus Fusarium was influenced by weather conditions in the analysed growing seasons. The applied fertilisation and the Nano-Gro ® organic growth stimulator exerted varied effects on FHB development and the biosynthesis of Fusarium mycotoxins (deoxynivalenol, nivalenol, zearalenone and fumonisins) in grain. The greatest reduction in deoxynivalenol and nivalenol concentrations was noted in 2013, and the levels of moniliformin were lower in treated samples than in absolute control (untreated) samples in both years of the study. The severity of FHB positively correlated with the concentrations of zearalenone, deoxynivalenol, nivalenol, and moniliformin in the grain samples. Greater accumulation of ergosterol was noted in the rye grain harvested in 2013 than in 2012, and fertiliser treatment led to higher ergosterol concentrations than did control treatment. KEY WORDS: deoxynivalenol; ergosterol; fertilisation with microelements; FHB; fumonisins; fungi; moniliformin; nivalenol; zearalenone Fusarium spp., common in all climate zones, can cause many diseases in cereal crops (1-4). These fungi are transmitted through soil and crop residues, compromising plant health as the infection spreads (5). Their pathogens attack plants from the early stages of development (BBCH 30) to maturity (BBCH 77 (3,(7)(8)(9)(10). Fusarium culmorum and F. avenaceum are the most common in Europe, including Poland (2-3, 11). According to Rintelen (12), F. poae and F. tricinctum are more common than F. culmorum and F. graminearum when dry and cool conditions that prevail during flowering are followed by a wet period at a later stage of development. Champeil et al. (13) found that the severity of FHB in wheat is largely determined by high relative humidity, wind, and rainfall during flowering and harvest.According to the literature (14-15), these pathogens differ in their responses to fertilisation with nitrate (N), phosphate (P), potassium (K), and microelements. High N fertilisation levels seem to increase the severity of infections with obligate pathogens but decrease the severity of infections with facultative parasites, including Fusarium spp. (16). Potassium reduces the host plant's susceptibility to infections, but only when applied at optimal rates. Unlike potassium's, phosphate's role in plant resistance to pathogens varies considerably. Microelements such as Cu, Zn, Mn, and B, in turn, seem to be involved in a plant's physiological...

show abstract

“…The determination of ergosterol content was conducted according to the procedure described previously [2,11]. The UltiMate 3000 RSLC chromatographic system from Dionex (Sunnyvale, CA, USA) was used.…”

Section: Ergosterol Content Using Liquid Chromatographymass Spectrometrymentioning

confidence: 99%

“…Ergosterol can be an indicator of fungal growth, also in food products [9][10][11]. Nevertheless, the enumeration of total number of fungi as their occurrence ascertainment is also a recognized and an important method in microbiological state evaluation of food products [12].…”

Section: Introductionmentioning

confidence: 99%

Quality assessment of goji fruits, cranberries, and raisins using selected markers

Jeszka-Skowron

Oszust

Zgoła‐Grześkowiak

et al. 2018

Eur Food Res Technol

Self Cite

View full text Add to dashboard Cite

The aim of the study was to determine the antioxidant activity, total phenolic content, and total carotenoid content of dried fruit extracts prepared from goji fruits, cranberries, and raisins. Ergosterol content of samples-as a marker of fungal contamination-was measured by LC-MS/MS technique. For the goji fruit samples, fungal community-level physiological profiling and fungal isolate catabolic diversity were tested to evaluate more precisely their fungal contamination. The highest antioxidant activity and total phenolic content were determined for dried goji fruits. Simultaneously, the dried goji fruits were contaminated by ergosterol − 3.8 µg g −1 of dried fruit and the bulk goji fruits were contaminated by heat-resistant fungi. The study of fungal community as a fungal contamination marker of dried goji fruits was performed by a combination of the Biolog FF plates and traditional culture methods which is the first application of this composite analytical approach. Ergosterol content could be an indicator of fungal growth on dried fruits. The Biolog FF microplates can be used as a quick, easy, cheap, and effective method for the detection of heat-resistant fungi in dried fruits.

show abstract

Can Ergosterol Be an Indicator ofFusariumFungi and Mycotoxins in Cereal Products?

Cited by 11 publications

References 3 publications

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

The effect of fertiliser treatments on the severity of Fusarium head blight and mycotoxin biosynthesis in winter rye

Quality assessment of goji fruits, cranberries, and raisins using selected markers

Contact Info

Product

Resources

About