High Throughput Enzymatic Enantiomeric Excess: Quick-<i>ee</i>

Lima, Maria L. S. O.; Gonçalves, Caroline C. S.; Barreiro, Juliana Cristina; Cass, Quézia B.; Marsaioli, Anita Jocelyne

doi:10.5935/0103-5053.20140282

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“…Due to its successful application to human MAO-A and MAO-B enzymatic activities (Lu et al 2008 ), 7-(3-aminopropoxy)coumarin ( 1 ) was selected as a fluorogenic probe for MAO enzymatic screening in microorganisms, based on our previous experience with whole-cell HTS experiments and analogous probes (Gonçalves and Marsaioli 2014 ; Lima et al 2015 ; Mantovani et al 2010 ). In this work, probe 1 was effective on selecting five fungi with possible MAO activity.…”

Section: Discussionmentioning

confidence: 99%

Monoamine oxidase and transaminase screening: biotransformation of 2-methyl-6-alkylpiperidines by Neopestalotiopsis sp. CBMAI 2030

Costa

Angelis

et al. 2017

Appl Microbiol Biotechnol

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High-throughput screening detected transaminases (TAs) and monoamine oxidases (MAOs) in fungi by applying a fluorogenic probe. Strains F026, F037, F041, F053, and F057 showed the highest enzymatic conversions (31, 60, 30, 40, and 32%, respectively) and where evaluated for their ability to transform piperidines. Strain F053 (Neopestalotiopsis sp. CBMAI 2030) revealed unusual enzymatic activity to deracemize 2-methyl-6-alkylpiperidines. Neopestalotiopsis sp. CBMAI 2030 was capable to convert 2-methyl-6-propylpiperidine, 2-methyl-6-butylpiperidine, and 2-methyl-6-pentylpiperidine in piperideine with 11, 14, and 24% conversion, respectively. The activity was enhanced by cultivating the fungus with 2-methyl-6-pentylpiperidine (38% conversion and 73% ee).Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-017-8389-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%