Improving Yield of 1,3-Diglyceride by Whole-Cell Lipase from<i>A. Niger</i>GZUF36 Catalyzed Glycerolysis via Medium Optimization

Li, Cuiqin; Li, Lanxiang; Zhou, Huanjing; Xia, Chaoshuang; He, Laping

doi:10.5935/0103-5053.20140261

Cited by 2 publications

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“…Understanding the interaction mechanisms of miRNAs provides more information on the regulation process of BLM gene expression at the post-transcriptional level. BBD has been applied to determine interactions between multiple factors in previous studies (31). Latin square design and the equivalent line method (2,30–32) are also able to calculate interaction effects, but the lower dose application of factors, more reasonable and three-dimensional arrangements are the main features of BBD which eliminates the dose superposing effects from multiple factors (in the study of factor interaction, multiple factors were used in one treatment group so that the dose of multiple factors will be elevated to create effect results).…”

Section: Discussionmentioning

confidence: 99%

miR‑27b‑3p and miR‑607 cooperatively regulate BLM gene expression by directly targeting the 3'‑UTR in PC3 cells

et al. 2019

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BLM RecQ like helicase (BLM) has a pivotal role in genetic recombination, transcription, DNA replication and DNA repair, which presents the possibility of using BLM as an anti-cancer target for treatment. However, the post-transcriptional control regulation of BLM gene expression is not fully understood and limits the application of drugs targeting BLM for carcinoma therapy in the future. MicroRNAs (miRNAs) inhibit gene expression through interaction with the 3′ untranslated region (3′-UTR) of mRNA at the post-transcriptional stage. Therefore, the current study screened for miRNAs that regulate BLM gene expression, with software predicting that miRNA (miR)-27b-3p, miR-607, miR-361-3p, miR-628-5p and miR-338-3p. BLM gene expression levels in the PC3 prostate cancer cell line and RWPE-2 normal prostate epithelium cell line were detected by reverse transcription-quantitative PCR. Additionally, BLM mRNA levels were following miRNA overexpression for 24 and 48 h. For further miRNA filtration and validation, a dual-luciferase reporter system and western blot analysis were performed, which demonstrated that miR-27b-3p and miR-607 reduce BLM gene expression by directly targeting the BLM mRNA 3′-UTR. A Box-Behnken design experiment suggested that miR-27b-3p and miR-607 have synergetic mutual effects on BLM gene expression. Finally, the suppressive effect of miR-27b-3p and miR-607 on PC3 cell proliferation, colony formation, migration and invasion indicated the benefit of studying BLM as a drug target in cancer. In conclusion, the findings of the current provide evidence that miR-27b-3p and miR-607 have an oncosuppressive function in PC3 cells and cooperatively downregulate BLM expression at the post-transcriptional level.

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Section: Discussionmentioning

confidence: 99%

miR‑27b‑3p and miR‑607 cooperatively regulate BLM gene expression by directly targeting the 3'‑UTR in PC3 cells

et al. 2019

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“…To complement Aspergillus lipase works, Li et al reported a novel method of synthesis of 1,3-diglyceride (1,3-DG), a healthy natural lipid which is extensively used in food processing and utilized as pharmaceutical intermediate. The synthesis of 1,3-DG via glycerolysis of triglyceride (TG) was catalyzed by whole-cell lipase from a new isolated strain, A. niger GZUF36 [111].…”

Section: Pharmaceutical Industrymentioning

confidence: 99%

Aspergillus Lipases: Biotechnological and Industrial Application

Contesini¹,

Calzado²,

Madeira³

et al. 2016

Fungal Metabolites

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Lipases are enzymes with remarkable properties and catalytic versatility. These proteins are capable of catalyzing hydrolytic and synthetic reactions, allowing the production of different compounds. Aspergillus are important producers of lipases, since they are able to secrete large amounts of these proteins to the extracellular media. Several studies have reported the importance of fermentation parameters as well as genetic engineering of Aspergillus strains in order to improve lipase production. Different Aspergillus species secrete lipases with interesting characteristics such as thermostability, stability in a wide pH range, stability in organic solvents, and enantioselectivity toward the substrate. The obtainment of lipases with highlighted characteristics for use in industry is the main focus of several studies. Such lipases can be obtained with screening of Aspergillus strains, protein engineering, and immobilization of lipases that can frequently improve thermostability and enantioselectivity. Among the applications of lipases from Aspergillus, there are studies on the improvement of sensorial properties of different products in the food industry, compatibility with detergents for removal of fat stains from fabrics, and the obtainment of enantiopure pharmaceuticals.

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Improving Yield of 1,3-Diglyceride by Whole-Cell Lipase fromA. NigerGZUF36 Catalyzed Glycerolysis via Medium Optimization

Cited by 2 publications

References 0 publications

miR‑27b‑3p and miR‑607 cooperatively regulate BLM gene expression by directly targeting the 3'‑UTR in PC3 cells

miR‑27b‑3p and miR‑607 cooperatively regulate BLM gene expression by directly targeting the 3'‑UTR in PC3 cells

Aspergillus Lipases: Biotechnological and Industrial Application

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