Leishmanicidal Activity of<i>Brosimum glaziovii</i>(Moraceae) and Chemical Composition of the Bioactive Fractions by Using High-Resolution Gas Chromatography and GC-MS

Coqueiro, Aline; Regasini, Luis Octávio; Leme, Gabriel Mazzi; Polese, Luciana; Nogueira, Camila Tita; Cistia, Mayara Lucia Del; Graminha, Márcia Aparecida Silva; Bolzani, Vanderlan S.

doi:10.5935/0103-5053.20140138

Cited by 5 publications

(5 citation statements)

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“…The use of the fingerstick test has shown good reproducibility (0.958 glucose, triglycerides 0.992, 0.940 total cholesterol) and high correlation with the laboratory method (glucose 0.952, 0.990 triglycerides, and total cholesterol 0.944). These results demonstrate the method is feasible for portable conducting research and as an alternative to the monitoring of metabolic disease and cardiovascular risk factors (COQUEIRO et al, 2014;EIZERIK, 2012).…”

Section: Discussionmentioning

confidence: 91%

The Relationship Between Risk Factors for Metabolic Syndrome in the Physically Active Elderly

Maciel¹,

Sonati

Modeneze

et al. 2015

Estud. interdiscip. envelhec.

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O envelhecimento tem um impacto significativo na diminuição de massa magra e do nível de atividade física, estando relacionada à redução na taxa metabólica basal e ao aumento de sobrepeso e obesidade em idosos. O objetivo desse estudo foi identificar correlações entre os fatores de risco da Síndrome Metabólica, a medição do Perímetro Abdominal (PA) e Índice de Massa Corporal (IMC) com os parâmetros sanguíneos de Glicemia em Jejum (GJ), Colesterol Total (CT) e Triglicerídeos (TG), em idosos ativos. Para cálculo do IMC foram coletadas medidas de peso e altura e adotados os critérios de classificação da Organização Mundial da Saúde (OMS), a medida da perímetro abdominal (PA) foi efetuada em centímetros. Os parâmetros sanguíneos de glicemia de jejum (GJ), colesterol total (CT) e triglicérides (TGL) foram avaliados pelo método de coleta de sangue na ponta de dedo com leitura em equipamento Accutrend Plus (Roche). Para análise dos resultados foi realizado o cálculo do coeficiente de correlação de Pearson entre as medidas antropométricas (variáveis independentes) e parâmetros sanguíneos (variáveis dependentes). Regressão linear simples foi aplicada sobre as variáveis significativas (0.05%). A avaliação do IMC mostrou que 71% dos idosos estavam acima do peso e 34% eram obesos. No ponto de corte recomendada, 57% da amostra ter sido indicado por PA. O teste de correlação mostraram evidências sobre a existência de uma associação significativa entre GJ e PA e também entre PA e TG comparado ao uso do IMC. A medição de PA parece ser uma indicação eficaz da relação entre os factores de risco para o SM e deve ser incorporada rotinas para avaliar idosos como um indicador de obesidade abdominal.

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Section: Discussionmentioning

confidence: 91%

The Relationship Between Risk Factors for Metabolic Syndrome in the Physically Active Elderly

Maciel¹,

Sonati

Modeneze

et al. 2015

Estud. interdiscip. envelhec.

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“…Several synthetic and natural products have been explored in order to discover new antileishmanial/antibacterial drugs [9][10][11][12][13][14][15][16][17][18][19][20][21]. In general, the process of drug discovery relies on the identification of bioactive compounds that target biochemical and metabolic pathways that are essential for pathogens viability and infectivity.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

et al. 2020

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Parasitic diseases are a neglected and serious problem, especially in underdeveloped countries. Among the major parasitic diseases, Leishmaniasis figures as an urgent challenge due to its high incidence and severity. At the same time, the indiscriminate use of antibiotics by the population is increasing together with resistance to medicines. To address this problem, new antibiotic-like molecules that directly kill or inhibit the growth of microorganisms are necessary, where antimicrobial peptides (AMPs) can be of great help. In this work, the ferrocene molecule, one active compound with low levels of in vivo toxicity, was coupled to the N-terminus of the RP1 peptide (derived from the human chemokine CXCL4), aiming to evaluate how this change modifies the structure, biological activity, and toxicity of the peptide. The peptide and the conjugate were synthesized using the solid phase peptide synthesis (SPPS). Circular dichroism assays in PBS showed that the RP1 peptide and its conjugate had a typical spectrum for disordered structures. The Fc-RP1 presented antiamastigote activity against Leishmania amazonensis (IC 50 = 0.25 μmol L -1 ). In comparison with amphotericin B, a second-line drug approved for leishmaniasis treatment, (IC 50 = 0.63 μmol L -1 ), Fc-RP1 was more active and showed a 2.5-fold higher selectivity index. The RP1 peptide presented a MIC of 4.3 μmol L -1 against S. agalactiae, whilst Fc-RP1 was four times more active (MIC = 0.96 μmol L -1 ), indicating that ferrocene improved the antimicrobial activity against Gram-positive bacteria. The Fc-RP1 peptide also decreased the minimum inhibitory concentration (MIC) in the assays against E. faecalis (MIC = 7.9 μmol L -1 ), E. coli (MIC = 3.9 μmol L -1 ) and S. aureus (MIC = 3.9 μmol L -1 ). The cytotoxicity of the compounds was tested against HaCaT cells, and no significant activity at the highest concentration tested (500 μg. mL -1 ) was observed, showing the high potential of this new compound as a possible new drug. The coupling of ferrocene also increased the vesicle permeabilization of the peptide, showing a direct relation between high peptide concentration and high carboxyfluorescein release, which indicates the action mechanism by pore formation on the vesicles. Several studies have shown that ferrocene destabilizes cell membranes through lipid peroxidation, leading to cell lysis. It is noteworthy that the Fc-RP1 peptide synthesized here PLOS ONE

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“…The cutaneous form of the disease is the most common manifestation, presenting an annual incidence of 0.7 to 1.3 million new cases [2,3]. The available therapeutic drugs for leishmaniasis treatment are still based on pentavalent antimonials, amphotericin B, miltefosine or paromomycin, which present several limitations such as toxicity, difficult route of administration and variable efficacy [4], and although many efforts have been made towards the discovery of new synthetic or natural antileishmanials [5][6][7][8][9][10][11][12], new therapeutic options are still needed. In recent years, photodynamic therapy (PDT) has been investigated as a potential alternative to control cutaneous leishmaniasis (CL) [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Solution-combustion synthesis of doped TiO 2 compounds and its potential antileishmanial activity mediated by photodynamic therapy

Lopera

Velásquez

Clementino

et al. 2018

Journal of Photochemistry and Photobiology B: Biology

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Photodynamic therapy has emerged as an alternative treatment for cutaneous leishmaniasis, and compounds with photocatalytic behavior are promising candidates to develop new therapeutic strategies for the treatment of this parasitic disease. Titanium dioxide TiO is a semiconductor ceramic material that shows excellent photocatalytic and antimicrobial activity under Ultraviolet irradiation. Due to the harmful effects of UV radiation, many efforts have been made in order to enhance both photocatalytic and antimicrobial properties of TiO in the visible region of the spectrum by doping or through modifications in the route of synthesis. Herein, Fe-, Zn-, or Pt- doped TiO nanostructures were synthesized by solution-combustion route. The obtained compounds presented aggregates of 100 nm, formed by particles smaller than 20 nm. Doping compounds shift the absorption spectrum towards the visible region, allowing production of reactive oxygen species in the presence of oxygen and molecular water when the system is irradiated in the visible spectrum. The Pt (EC = 18.2 ± 0.8 μg/mL) and Zn (EC = 16.4 ± 0.3 μg/mL) -doped TiO presented the higher antileishmanial activities under visible irradiation and their application as photosensitizers in photodynamic therapy (PDT) strategies for the treatment of cutaneous leishmaniasis should be considered.

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Leishmanicidal Activity ofBrosimum glaziovii(Moraceae) and Chemical Composition of the Bioactive Fractions by Using High-Resolution Gas Chromatography and GC-MS

Cited by 5 publications

References 0 publications

The Relationship Between Risk Factors for Metabolic Syndrome in the Physically Active Elderly

The Relationship Between Risk Factors for Metabolic Syndrome in the Physically Active Elderly

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

Solution-combustion synthesis of doped TiO 2 compounds and its potential antileishmanial activity mediated by photodynamic therapy

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