A Chemometric Model Applied to Fatty Acid Determination in Blood

Lopes, Thiago Beirigo; Rimland, Casey A.; Nagassaki, Sabrina; Geloneze, Bruno; Marsaioli, Anita J.

doi:10.5935/0103-5053.20130201

Cited by 3 publications

(4 citation statements)

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“…The lipids were extracted from 100 lL of plasma applying a modified Folch's methodology (Folch et al, 1957;Iverson et al, 2001). The FA were converted to their respective FA methyl esters (FAME) as described by Croset et al (2000), and the selected ion monitoring chromatograms were obtained as described by Lopes et al (2013). The experiments were carried out using an Agilent-6890 series equipped with a Hewlett Packard-5973 mass selective detector.…”

Section: Gc-ms Acquisition and Processingmentioning

confidence: 99%

Blood Metabolome Changes Before and After Bariatric Surgery: A¹H NMR-Based Clinical Investigation

Lopes

Geloneze²,

Pareja³

et al. 2015

OMICS: A Journal of Integrative Biology

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Excessive body fat and obesity have adverse health effects and result in significant morbidity such as type 2 diabetes mellitus. The health burden of obesity can be reduced with the Roux-en-Y gastric bypass (RYGB) weight-loss bariatric surgery. Little is known on the molecular changes that occur at the metabolome level before and after bariatric surgery, with a view to clinical biomarker development. Hence, we employed a metabolomics approach in 10 obese diabetic patients who underwent bariatric surgery. Metabolomics data were obtained by T2- and diffusion-edited hydrogen nuclear magnetic resonance ((1)H NMR) spectra to monitor the metabolic and lipoprotein profiles, and gas chromatography-mass spectrometry (CG-MS) to access the fatty acid profile before and 12 months after RYGB. Using hierarchical partial least squares discriminant analysis, we found that RYGB induces several key metabolic alterations associated with glucose homeostasis, as well as fatty acid and amino acid metabolism. The levels of lactate (Krebs' intermediate cycle) decreased after RYGB. The leucine, isoleucine, valine, lactate, and glucose levels were higher in the samples before RYGB (p<0.05). Additionally, the levels of very low-density lipoprotein, unsaturated lipids, and N-acetyl-glycoprotein were higher before RYGB. By contrast, levels of the high-density lipoprotein and phosphatidylcholine were higher after bariatric surgery. These results collectively offer important holistic integrative biology data to develop future clinically relevant metabolomics biomarkers related to bariatric surgery in connection with obesity.

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Section: Gc-ms Acquisition and Processingmentioning

confidence: 99%

Blood Metabolome Changes Before and After Bariatric Surgery: A¹H NMR-Based Clinical Investigation

Lopes

Geloneze²,

Pareja³

et al. 2015

OMICS: A Journal of Integrative Biology

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“…In the literature, most fatty acid analyses are based on a methylation derivatization (the derivatives are known as FAMEs; fatty acid methyl esters), followed by GC-MS or GC-FID [26,52,55,56]. This derivatization reaction is well-documented and its advantages and disadvantages have been previously discussed in the literature [52].…”

Section: Discussionmentioning

confidence: 99%

“…As the value of the quadratic coefficient was small, if the calibration range was reduced by removing the two highest concentrations (50 and 20 mg/L points), the calibration would become linear. However, based on the experiments, it was decided to keep the quadratic calibrations in order to enable the quantification of a wider range of concentrations [55,57]. Dilution of the sample is another practical approach worth considering when the expected concentrations are approximatively known and/or when they are presumed to be high.…”

Section: Discussionmentioning

confidence: 99%

A Gas Chromatography-Mass Spectrometry Method for the Determination of Fatty Acids and Sterols in Yeast and Grape Juice

et al. 2021

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Lipids are essential components of all living cells. In an oenological context, the supply of unsaturated lipids in grape juice allows the yeasts to grow and ferment, despite very low levels of oxygen. The current study proposes a systematic optimization procedure for the analysis of fatty acids and sterols relevant to the grape fermentation process, including both extracellular and intracellular (i.e., yeast cells) lipids. Even though it was extensive, the sample preparation yielded reproducible results for all compounds of interest. The stability of the analyzed compounds was also tested to offer some implementation flexibility for the extensive procedure. The performance parameters (i.e., selectivity, linearity, limit of detection and quantitation, accuracy, and precision) indicated that the method was suitable for future practical implementation. The proof of concept also suggests that the list of compounds of interest can be expanded if additional peaks are identified. Given the large variation in concentrations, the dilution of the matrix needs to be carefully considered in order to ensure that the lipids of interest are still within the dynamic range and not below the limit of detection and/or quantification.

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“…O resultado da má absorção de gordura é um balanço energético negativo que produz uma perda de peso subtraída por placebo em de 2,6% (DAVIDSON et al, 1999;KHERA et al, 2016). A perda modesta de peso, apesar de associada com melhoras metabólicas (ZELBER-SAGI et al, 2006, LOPES, GELONEZE et al, 2013, ainda está associada com problemas clínicos, tais quais a defecação espontânea, consistência fecal anormais devido à esteatorréia, e também com deficiências em vitaminas lipossolúveis (MELIA et al, 1996;MCDUFFIE et al, 2002). A utilização clínica de orlistate segue ativa, mas cada vez menos frequente.…”

Section: Inibição Da Lipase Intestinal Com Orlistateunclassified

Tratamento farmacológico da obesidade: passado, presente e futuro.

Geloneze

2019

HU Rev

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O tratamento da obesidade deve ser baseado na percepção de que a obesidade é uma enfermidade crônica, neuroquímica e recidivante. Sendo assim, a combinação de eficácia, sustentabilidade de longo prazo e perfil de segurança de excelência devem nortear a utilização dos medicamentos disponíveis assim como das novas moléculas vindouras. No momento, a liraglutida parece ser a mais completa droga para a obesidade como doença crônica, mas certamente deverá ser superada pelos novos peptídeos de múltiplo agonismo e/ou pela combinação de várias medicações ainda não disponíveis em baixas dosagens para pacientes precisa e individualmente selecionados.

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A Chemometric Model Applied to Fatty Acid Determination in Blood

Cited by 3 publications

References 1 publication

Blood Metabolome Changes Before and After Bariatric Surgery: A¹H NMR-Based Clinical Investigation

Blood Metabolome Changes Before and After Bariatric Surgery: A¹H NMR-Based Clinical Investigation

A Gas Chromatography-Mass Spectrometry Method for the Determination of Fatty Acids and Sterols in Yeast and Grape Juice

Tratamento farmacológico da obesidade: passado, presente e futuro.

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A Chemometric Model Applied to Fatty Acid Determination in Blood

Cited by 3 publications

References 1 publication

Blood Metabolome Changes Before and After Bariatric Surgery: A1H NMR-Based Clinical Investigation

Blood Metabolome Changes Before and After Bariatric Surgery: A1H NMR-Based Clinical Investigation

A Gas Chromatography-Mass Spectrometry Method for the Determination of Fatty Acids and Sterols in Yeast and Grape Juice

Tratamento farmacológico da obesidade: passado, presente e futuro.

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Blood Metabolome Changes Before and After Bariatric Surgery: A¹H NMR-Based Clinical Investigation

Blood Metabolome Changes Before and After Bariatric Surgery: A¹H NMR-Based Clinical Investigation