Microwave-Assisted Extraction Combined with HPLC-MS/MS for Diagnosis of Fungal Contamination in Building Materials

Horbik, Daria; Łowińska‐Kluge, Aldona; Górski, Zbigniew; Stanisz, Ewa; Zgoła‐Grześkowiak, Agnieszka

doi:10.5935/0103-5053.20130191

Cited by 5 publications

(19 citation statements)

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“…Ergosterol was determined according to the procedure described previously . Briefly, 200 mg of sample was placed in a 12 mL glass culture tube, then 2 mL of methanol was added, followed by 0.5 mL of 2 mol L −1 NaOH.…”

Section: Methodsmentioning

confidence: 99%

“…Ergosterol was determined according to the procedure described previously. 20,29 Briefly, 200 mg of sample was placed in a 12 mL glass culture tube, then 2 mL of methanol was added, followed by 0.5 mL of 2 mol L −1 NaOH. The tube was sealed with a rubber-lined screw cap and placed in a 200 mL screw-capped high-density polyethylene bottle (Bel-Art Products, Wayne, NJ, USA).…”

Section: Analysis Of Ergosterol and Mycotoxin Contents Using Liquid Cmentioning

confidence: 99%

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Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

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Section: Methodsmentioning

confidence: 99%

Section: Analysis Of Ergosterol and Mycotoxin Contents Using Liquid Cmentioning

confidence: 99%

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

et al. 2017

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“…7,23,27 Efficiency of tested antifungal agent on the basis of bioluminescence measurement was also studied. Influence of biocorrosion on changes of selected physico-chemical properties of infected materials was discussed on the basis of pH and EDS measurements.…”

Section: Assessment Of the Fungi Activity On The Tested Materialsmentioning

confidence: 99%

A comprehensive study on the risk of biocorrosion of building materials

Łowińska‐Kluge

Horbik

Zgoła‐Grześkowiak

et al. 2016

Corrosion Engineering, Science and Technology

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The fungal genera are known for their high ability to biodeteriorate construction materials. Weight loss and release of calcium are observed when materials, especially concrete, are exposed to fungi. The paper presents the procedures enabling to assess the risk of biocorrosion of buildings at various stages of its development. The presented results lead to selection of effective analytical methodology for this propose. The study was conducted on materials commonly used as construction materials such as granite, ceramic, concrete and plaster. Surfaces of the tested samples were fouled by three selected fungal genera. Changes in properties of the materials due to interaction of fungi have been studied with the use of ergosterol content measurements, microscopic picturing, bioluminescence imaging, measurements of pH and X-ray spectroscopy analysis. Particular attention was paid to propose an appropriate method of estimating the effect of fungi on the surface of building materials using advanced analytical techniques.

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“…Ergosterol can be an indicator of fungal growth, also in food products [9][10][11]. Nevertheless, the enumeration of total number of fungi as their occurrence ascertainment is also a recognized and an important method in microbiological state evaluation of food products [12].…”

Section: Introductionmentioning

confidence: 99%

Quality assessment of goji fruits, cranberries, and raisins using selected markers

Jeszka-Skowron

Oszust

Zgoła‐Grześkowiak

et al. 2018

Eur Food Res Technol

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The aim of the study was to determine the antioxidant activity, total phenolic content, and total carotenoid content of dried fruit extracts prepared from goji fruits, cranberries, and raisins. Ergosterol content of samples-as a marker of fungal contamination-was measured by LC-MS/MS technique. For the goji fruit samples, fungal community-level physiological profiling and fungal isolate catabolic diversity were tested to evaluate more precisely their fungal contamination. The highest antioxidant activity and total phenolic content were determined for dried goji fruits. Simultaneously, the dried goji fruits were contaminated by ergosterol − 3.8 µg g −1 of dried fruit and the bulk goji fruits were contaminated by heat-resistant fungi. The study of fungal community as a fungal contamination marker of dried goji fruits was performed by a combination of the Biolog FF plates and traditional culture methods which is the first application of this composite analytical approach. Ergosterol content could be an indicator of fungal growth on dried fruits. The Biolog FF microplates can be used as a quick, easy, cheap, and effective method for the detection of heat-resistant fungi in dried fruits.

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Microwave-Assisted Extraction Combined with HPLC-MS/MS for Diagnosis of Fungal Contamination in Building Materials

Cited by 5 publications

References 0 publications

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

Positive and negative aspects of green coffee consumption – antioxidant activity versus mycotoxins

A comprehensive study on the risk of biocorrosion of building materials

Quality assessment of goji fruits, cranberries, and raisins using selected markers

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