Investigation of the Interaction of Sertraline with Calf Thymus DNA by Spectroscopic Methods

Dorraji, Parisa Seyed; Jalali, Fahimeh

doi:10.5935/0103-5053.20130123

Cited by 9 publications

(8 citation statements)

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“…The thermodynamic parameters indicated that the interaction between [DEX] and DNA was spontaneous and hydrophobic interactions were present as major forces in the DEX-DNA binding that dominates the minor groove of binding. Similar observation was found in some other studies (Dorraji et al, 2013;Lv et al, 2014;Sultana et al, 2013).…”

Section: Discussionsupporting

confidence: 80%

Study of Interaction of Dextromethorphan Hydrobromide with Deoxyribonucleic Acid by Fluorescence Quenching

et al. 2016

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Interactions with many clinically active therapeutic agents with deoxyribonucleic acid (DNA) are well studied and it expedites deciphering the structure of DNA and to investigate the pathological implication of those molecules in a living organism. The interaction of dextromethorphan hydrobromide (DEX) with calf thymus DNA (ct DNA) was studied employing UV absorption and fluorescence spectroscopic techniques. The binding affinity of DEX to DNA was calculated at different temperatures and the stoichiometry of binding was characterized to be about 1 dextromethorphan molecule per nucleotide. Hypochromic effect was found in the absorption spectra of dextromethorphan, and its wavelength had no shift in the presence of DNA indicating external binding mode of dextromethorphan to DNA. Quenching constants 3532 L/ mol and 12446L/ mol at 298 K and 308 K respectively with correlation co-efficient of 0.974 and 0.976, using SternVolmer equation and the quenching mechanism was found to be dynamic. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DEX in the presence of DNA, indicating the interaction between DEX and DNA. Based on this, hydrophobic interaction were found to play the dominating role in DEX-DNA binding and those binding forces also indicate the binding site of dextromethorphan to be in the minor groove of DNA.

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Section: Discussionsupporting

confidence: 80%

Study of Interaction of Dextromethorphan Hydrobromide with Deoxyribonucleic Acid by Fluorescence Quenching

et al. 2016

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“…Circular dichroism spectroscopy is valuable to determine the mobility and orientation of intercalated ligands in dsDNA. The CD spectrum of dsDNA shows a positive peak at near 275 nm related to base stacking and a negative peak at near 245 nm related to the helical geometry of BDNA respectively 26 , 27 . On addition of OEO/thymol to a solution of DNA, slight changes in CD spectrum were detected.…”

Section: Resultsmentioning

confidence: 99%

In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA

Jamali

Kavoosi

Safavi

et al. 2018

Sci Rep

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Oliveria decumbens is an Iranian endemic plant used extensively in traditional medicine. Recently, some studies have been performed on biological effects of Oliveria essential oil (OEO). However, to our knowledge, the anticancer activity of OEO has not been reported. Based on our GC/MS analysis, the basic ingredients of OEO are thymol, carvacrol, p-cymene and γ-terpinene. Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. Despite having a limited effect on L929 normal cells, OEO/thymol induced cytotoxicity in MDA-MB231 breast cancer monolayers (2D) and to a lesser extent in MDA-MB231 spheroids (3D). Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. Indeed, OEO/thymol increased the Reactive Oxygen Species (ROS) level leading to mitochondrial membrane potential (MMP, ΔΨm) loss, caspase-3 activation and DNA damage caused S-phase cell cycle arrest. Furthermore, immunoblotting studies revealed the activation of intrinsic and maybe extrinsic apoptosis pathways by OEO/thymol. Additionally, in-vitro experiments, indicated that OEO/thymol interacts with DNA via minor grooves confirmed by docking method. Altogether, our reports underlined the potential of OEO to be considered as a new candidate for cancer therapy.

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“…The calculated binding constants for the Iso-DNA systems are slightly lower than those calculated by absorption spectroscopy due to the difference in the method used [ 37 ]. However, the magnitude of the evaluated association constants still confirms the higher affinity of Isoc, with respect to Isoa and Isob, to the DNA base pairs, which is in agreement with the data derived from UV-Vis spectroscopy.…”

Section: Resultsmentioning

confidence: 99%

Interactions of Isophorone Derivatives with DNA: Spectroscopic Studies

et al. 2015

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Interactions of three new isophorone derivatives, Isoa Isob and Isoc with salmon testes DNA have been investigated using UV-Vis, fluorescence and circular dichroism spectroscopic methods. All the studied compounds interact with DNA through intercalative binding mode. The stoichiometry of the isophorone/DNA adducts was found to be 1:1. The fluorescence quenching data revealed a binding interaction with the base pairs of DNA. The CD data indicate that all the investigated isophorones induce DNA modifications.

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Investigation of the Interaction of Sertraline with Calf Thymus DNA by Spectroscopic Methods

Cited by 9 publications

References 0 publications

Study of Interaction of Dextromethorphan Hydrobromide with Deoxyribonucleic Acid by Fluorescence Quenching

Study of Interaction of Dextromethorphan Hydrobromide with Deoxyribonucleic Acid by Fluorescence Quenching

In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA

Interactions of Isophorone Derivatives with DNA: Spectroscopic Studies

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