Potential Diagnostic Assay for Cystinuria by Capillary Electrophoresis Coupled to Mass Spectrometry

Barbosa, C.; Gonçalves, Norberto S.; Bechara, Etelvino José Henriques; Assunção, Nilson Antônio

doi:10.5935/0103-5053.20130085

Cited by 2 publications

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“…The goal of metabolomics is to study changes in the metabolome, which is composed of all biomolecules in a biological system of molecular mass up to 1,500 Da containing different chemical functions and variable concentration ranges found in a biological system (Fiehn, 2001;Barbosa et al, 2012;Viant et al, 2017). Several analytical platforms have been used for metabolomics analyses, such as gas chromatography-mass spectrometry (GC-MS) (Jonsson et al, 2004), liquid chromatography-mass spectrometry (LC-MS) (Cubbon et al, 2010), capillary electrophoresis-mass spectrometry (CE-MS) (Barbosa et al, 2013), and nuclear magnetic resonance (NMR) (Du et al, 2019), but LC-MS has been widely used for biological analysis and life science studies. Performing a metabolomics study in cellular culture (in vitro studies) avoids invasive techniques for obtaining biological samples.…”

Section: Introductionmentioning

confidence: 99%

Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples

et al. 2022

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Cardiovascular diseases (CVDs) are noncommunicable diseases known for their complex etiology and high mortality rate. Oxidative stress (OS), a condition in which the release of free radical exceeds endogenous antioxidant capacity, is pivotal in CVC, such as myocardial infarction, ischemia/reperfusion, and heart failure. Due to the lack of information about the implications of OS on cardiovascular conditions, several methodologies have been applied to investigate the causes and consequences, and to find new ways of diagnosis and treatment as well. In the present study, cardiac dysfunction was evaluated by analyzing cells’ alterations with untargeted metabolomics, after simulation of an oxidative stress condition using hydrogen peroxide (H2O2) in H9c2 myocytes. Optimizations of H2O2 concentration, cell exposure, and cell recovery times were performed through MTT assays. Intracellular metabolites were analyzed right after the oxidative stress (oxidative stress group) and after 48 h of cell recovery (recovery group) by ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) in positive and negative ESI ionization mode. Significant alterations were found in pathways such as “alanine, aspartate and glutamate metabolism”, “glycolysis”, and “glutathione metabolism”, mostly with increased metabolites (upregulated). Furthermore, our results indicated that the LC-MS method is effective for studying metabolism in cardiomyocytes and generated excellent fit (R2Y > 0.987) and predictability (Q2 > 0.84) values.

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Section: Introductionmentioning

confidence: 99%

Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples

et al. 2022

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“…Yet another benefit of urine collection is that it is not invasive and large volumes of samples can be easily collected. Through these factors, urine has become a promising fluid for use in metabolic studies (Barbosa, Gonçalves, Bechara, & Assunção, ; Gowda et al, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Yet another benefit of urine collection is that it is not invasive and large volumes of samples can be ABBREVIATIONS: 5p minus, 5p−; ultra-performance liquid chromatography mass spectrometry, UPLC-MS/MS; alanine, Ala; arginine, Arg; asparagine, Asn; aspartate, Asp; citrulline, Cit; glutamine, Gln; glutamic acid, Glu; glycine, Gly; histidine, His; isoleucine, Ile; leucine, Leu; lysine, Lys; methionine, Met; ornithine, Orn; phenylalanine, Phe; proline, Pro; serine, Ser; threonine, Thr; tryptophan, Trp; tyrosine, Tyr; valine, Val; acetylornithine, AcOrn; asymmetric dimethylarginine, Adma; symmetric dimethylarginine, Sdma; alpha-aminoadipic acid, alpha AAA; methionine sulfoxide, MetO; nitro-tyrosine, N-Tyr; 3hydroxybutyrylcarnitine, C4-OH; trans-4-hydroxyproline, T4 OH Pro; serotonin, 5HT; electrospray ionization, ESI; standard deviation, SD; Metabolite Set Enrichment Analysis, MSEA; tricarboxylic acid cycle, TCA cycle easily collected. Through these factors, urine has become a promising fluid for use in metabolic studies (Barbosa, Gonçalves, Bechara, & Assunção, 2013;Gowda et al, 2008;Wang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Targeted analysis reveals alteration in pathway in 5p minus individuals

Furtado

Leite

Jedlicka

et al. 2020

Biomedical Chromatography

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Cri du Chat or 5p minus (5p−) syndrome is characterized by a deletion located on the chromosome 5 short (−p) arm and has an incidence rate of 1 in 50,000 individuals worldwide. This disease manifests in disturbances across a range of systems biochemicals. Therefore, a targeted metabolomics analysis was evaluated in patients with 5p− syndrome to help unravel the biochemical changes that occur in this disease. Urine samples were collected from people of both sexes aged 1–38 years old and analyzed by ultra‐performance liquid chromatography coupled to mass spectrometry. Student' statistical test, metabolomic pathway analysis and metabolite set enrichment analysis were applied to the data. Alterations of some amino acids and amine biogenics levels were found in Cri du Chat Syndrome individuals. The alteration of most of these metabolites is associated with energy recuperation and glycolysis. In general, we found the catabolism of some metabolic pathways to be affected in 5p− patients.

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Potential Diagnostic Assay for Cystinuria by Capillary Electrophoresis Coupled to Mass Spectrometry

Cited by 2 publications

References 0 publications

Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples

Comprehending Cardiac Dysfunction by Oxidative Stress: Untargeted Metabolomics of In Vitro Samples

Targeted analysis reveals alteration in pathway in 5p minus individuals

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