Detection of podocyturia in patients with lupus nephritis

Sabino, Amélia Rodrigues Pereira; Teixeira, Vicente de Paulo Castro; Nishida, Sônia K.; Sass, Nelson; Mansur, Juliana; Kirsztajn, Gianna Mastroianni

doi:10.5935/0101-2800.20130043

Cited by 12 publications

(9 citation statements)

References 18 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recently published study by our group likewise did not observe such association. 20 Although a statistical relationship between proteinuria and podocyturia has not been demonstrated, we cannot rule out a possible association between these parameters, as described by certain authors. 4,7 It is possible that other factors, such as size sample, affected the result.…”

Section: Discussionmentioning

confidence: 75%

“…20 While the referred study was predominantly methodological, the present one has its focus on clinical application of the laboratory methods tested and previously approved. Here, in a new group of patients, we have also emphasized the use of a widely known system of classification of lupus activity (BILAG) to facilitate understanding and comparison.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Is there a role for urinary podocyte excretion assessment in lupus nephritis?

et al. 2016

Self Cite

View full text Add to dashboard Cite

Objective To establish the occurrence and intensity of podocyturia and its relation to grade of disease activity, as defined by clinical and laboratory criteria. Methods Prospective, cross-sectional study involving 50 patients with lupus nephritis and 29 controls, which had podocyturia levels determined from random urine samples using an immunofluorescence technique. Disease activity was graded by BILAG (renal criteria) and an additional system used in the service (S2). Results Fifty patients with lupus nephritis (WHO classes III, IV and V), with a median age of 37 years, were evaluated. Of these, 86.5% were female, and 52% were BILAG A. Podocyturia quantification in the lupus nephritis and control groups differed significantly (p ¼ 0.009). This score was higher in relation to classes III, IV and V. The correlation with C3 consumption was stronger (p ¼ 0.011) than with C4. The highest levels were found in the most active groups (A and B of BILAG and S2). Lower podocyturia correlated with a lower dose of prednisone. There was no association with the intensity of proteinuria, hematuria or pyuria, serum creatinine levels, among others. Conclusions Podocyturia assessment, which was performed by immunofluorescence in this study, can be used as an indicator of disease activity with the advantage of being a urinary biomarker. The levels proved to be higher in patients with lupus nephritis than in the controls and were particularly higher in class IV. ARTICLE HISTORY

show abstract

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Is there a role for urinary podocyte excretion assessment in lupus nephritis?

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Segundo o número de podócitos na urina, foram assumidos dois grupos: grupo GN (função glomerular normal), com até 100 podócitos, e grupo GP (provável glomerulopatia), com mais de 100 podócitos. Esse ponto de corte foi baseado nos achados de estudo realizado por Sabino et al 8 . A dosagem de creatinina na urina foi realizada mediante o método do picrato alcalino.…”

Section: Métodosunclassified

“…O entendimento da biologia celular dos podócitos parece ser decisivo tanto para o diagnóstico molecular de doença glomerular como para o desenvolvimento de terapias mais específicas para o futuro 6 . A podocitúria parece ser um marcador não invasivo de doença ativa ou em evolução, e sua presença marcante na urina poderia ser um fator comum em situações que evoluem com proteinúria, como glomerulonefrites crô-nicas, e até mesmo um marcador da gravidade de doença preexistente 7,8 . Considerando que na pré-eclâmpsia (PE) exista risco potencial para dano renal definitivo, alguns autores identificaram em mulheres com PE perda urinária significativa de podócitos, ainda que não tenha sido definido um padrão passível de ser adotado como um fator prognóstico, como um indicador de problemas imediatos ou futuros 9,10 .…”

Section: Introductionunclassified

Podocitúria em gestantes hipertensas crônicas pode predizer dano renal?

Roberto¹,

Facca²,

Sato³

et al. 2015

Rev. Bras. Ginecol. Obstet.

Self Cite

View full text Add to dashboard Cite

Resumo OBJETIVO: Avaliar a presença de podocitúria em gestantes hipertensas crônicas no terceiro trimestre da gestação e a associação com doença renal. MÉTODOS: Estudo observacional descritivo em uma amostra de conveniência de 38 gestantes hipertensas crônicas. Os podócitos foram marcados com técnica de imunofluorescência indireta com antipodocina e diamidino-fenilindol (DAPI). A contagem foi feita a partir de 30 campos analisados de forma aleatória, corrigida pela creatinina urinária (podócitos/mg de creatinina). Foram assumidos dois grupos: grupo GN (função glomerular normal), com até 100 podócitos, e grupo GP (provável glomerulopatia), com mais de 100 podócitos. A dosagem de creatinina foi realizada com uso da técnica do picrato alcalino. As variáveis de análise foram o índice de massa corpórea, a idade gestacional na coleta, a pressão arterial sistólica e a pressão arterial diastólica no momento da coleta. Para a análise dos dados, foi utilizado o programa SPSS -versão 16.0. (IBM -USA). Nas análises estatísticas, foi utilizado o teste do χ 2 , sendo consideradas diferenças significantes valores de p<0,05. RESULTADOS: A contagem de podócitos no grupo GN teve mediana de 20,3 (0,0 a 98,1), e no grupo GP, de 176,9 (109,1 a 490,6). A média do índice de massa corpórea foi 30,2 kg/m 2 (DP=5,6), a média da idade gestacional foi de 35,1 semanas (DP=2,5), a mediana da pressão arterial sistólica foi de 130,0 mmHg (100,0-160,0) e a mediana da pressão arterial diastólica de 80,0 mmHg (60,0-110,0). Não houve correlação significativa entre podocitúria e índice de massa corpórea (p=0,305), idade gestacional na coleta (p=0,392), pressão arterial sistólica (p=0,540) e pressão arterial diastólica (p=0,540). CONCLUSÕES: Não foi identificado um padrão de podocitúria compatível com a presença de glomerulopatia ativa, ainda que algumas das gestantes (15,8%) tenham exibido perda podocitária expressiva. Consideramos ser prematuro recomendar para a prática clínica rotineira a incorporação da pesquisa de podocitúria ao longo do pré-natal de gestantes hipertensas crônicas. Abstract PURPOSE:To evaluate the presence of podocyturia in chronic hypertensive pregnant women in the third trimester of pregnancy and its possible association with renal disease. METHODS: This was an observational study of a convenience sample of 38 chronic hypertensive pregnant women. The podocytes were labeled by the indirect immunofluorescence technique with anti-podocin and diamidino-phenylindole (DAPI). The count was made on 30 random fields analyzed and corrected according to urinary creatinine (podocytes/mg creatinine). The patients were assigned to two groups: NG (normal glomerular function), up to 100 podocytes, and GP (probable glomerulopathy), more than 100 podocytes. Urinary creatinine was measured by the alkaline picrate method. The variables analyzed were body mass index, gestational age, and systolic and diastolic blood pressure at the time of sample collection. Data were analyzed using the SPSS -version 16.0 (IBM -USA). Statistical analysis was perf...

show abstract

“…Following our standardized technique, podocytes were counted in 10 randomly chosen 20× fields and the average of the counted podocytes in the microscopy fields was considered as the final count for each subject. Results were corrected based on the urinary creatinine concentration of the initial urinary volume of 20 ml employed for podocyte counting [ 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

Increased urinary CD80 excretion and podocyturia in Fabry disease

et al. 2016

View full text Add to dashboard Cite

BackgroundCertain glomerulopathies are associated with increased levels of CD80 (B7-1). We measured the urinary excretion of CD80, podocyturia and proteinuria in controls and in subjects with Fabry disease either untreated or on enzyme replacement therapy (ERT).MethodsCross-sectional study including 65 individuals: controls (n = 20) and Fabry patients (n = 45, 23 of them not on ERT and 22 on ERT). Variables included age, gender, urinary protein/creatinine ratio (UPCR), estimated glomerular filtration rate (eGFR), urinary uCD80/creatinine ratio (uCD80) and podocyturia. CD80 mRNA expression in response to lyso-Gb3, a bioactive glycolipid accumulated in Fabry disease, was studied in cultured human podocytes.ResultsControls and Fabry patients did not differ in age, eGFR and gender. However, UPCR, uCD80 and podocyturia were significantly higher in Fabry patients than in controls. As expected, Fabry patients not on ERT were younger and a higher percentage were females. Non-ERT Fabry patients had less advanced kidney disease than ERT Fabry patients: UPCR was lower and eGFR higher, but uCD80 and podocyturia did not differ between non-ERT or ERT Fabry patients. There was a significant correlation between uCD80 and UPCR in the whole population (r 0.44, p 0.0005) and in Fabry patients (r 0.42, p 0.0046). Lyso-Gb3 at concentrations found in the circulation of Fabry patients increased uCD80 expression in cultured podocytes.ConclusionsFabry disease is characterized by early occurrence of increased uCD80 excretion that appears to be a consequence of glycolipid accumulation. The potential for uCD80 excretion to reflect early, subclinical renal Fabry involvement should be further studied.

show abstract

Detection of podocyturia in patients with lupus nephritis

Abstract: Our findings show that podocyturia with anti-podocin could be useful in monitoring disease activity in LN patients.

Cited by 12 publications

References 18 publications

Is there a role for urinary podocyte excretion assessment in lupus nephritis?

Is there a role for urinary podocyte excretion assessment in lupus nephritis?

Podocitúria em gestantes hipertensas crônicas pode predizer dano renal?

Increased urinary CD80 excretion and podocyturia in Fabry disease

Contact Info

Product

Resources

About