Interaction of Chalcones With Ct-Dna by Spectrophotometric Analysis and Theoreticalsimulations

Abstract

publicado na web em 08/07/2016 Chalcones are open chain molecules precursors of flavonoids and isoflavonoids, found spread in edible plants. Because they are easily accessible trough Claisen Shmidt condensation, a great variety of derivatives are available. They have also shown potential in pharmacological and biological applications. It is known that chalcone derivatives display a role in the treatment of complex diseases such as cancer, among others, where the DNA is considered as the target for the action of these kinds of compounds. This action is commonly explained as the inhibition of the DNA replications and transcriptions through interactions. However, not conclusive associations between these DNA-Drug interactions and toxicity have been found. This research focuses on the capacity of a chalcone`s family to interact with DNA. Therefore, the binding constants for each compounds with Calf Thymus DNA [CT-DNA] were determined by spectrophotometric titration at room temperature. In addition, the effect of increasing the chalcone`s concentration over the relative viscosity of CT-DNA at room temperature was assessed. On the other hand, with the aim to find the optimal DNAchalcone configurations, as well as consistently predict their binding, a computational work was undertaken. To accomplish these goals within a reasonable time framework, an empirical scoring function (AScore) and a docking engine (ShapeDock) were performed using the ArgusLab package. The results of viscosity and docking measurement provided structural insights which suggest that chalcones bind with DNA via interaction as well as intercalation. The presence of interactions is also evidenced by the spectrophotometric study which showed luminescence quenching of the chalcones upon interaction with CT-DNA.

Fabrication of Transparent Thin Film of Octahedral Molybdenum Metal Clusters by Electrophoretic Deposition

“…The pathways of the valence electrons are metal-metal charge transfer and metal-ligand-metal charge transition through the localized orbital of the metallic core and ligands. 13,70 The localized orbitals may stably store the valence electrons. When the amount of the Mo 6 clusters on the ITO glass increases, it achieves a close-packed assembly of the localized orbitals between the Mo 6 clusters.…”

The luminescent Chalcones. Its potential use as a luminescent and antitumoral agents

“…Chalcones were prepared following a previously described methodology (8,11,16) by adding, in a dropwise manner, a solution of the corresponding substituted benzaldehyde, (7.34 RESULTADOS: Se observaron efectos citotóxicos sobre las líneas celulares del tipo HepG2 y EA.hy926. Dado que la estructura de la 2´-hidroxichalcona puede ser usada como sonda fluorescente, estos compuestos pueden ser útiles en el diagnóstico del cáncer y la localización del tumor, ya que pueden permitir la observación a través de la fluorescencia y la regresión del tumor durante el tratamiento, por lo que son candidatas potenciales como nuevos agentes anticancerígenos que podrían actuar sobre hepatomas humanos.…”

“…The wide range of biological activity associated with many Chalcone derivatives isolated from natural products has stimulated interest in developing synthetic strategies to synthesize heterocyclic systems based on the chalcone core. Along with efficient methodologies implemented for their preparation, investigations have also focused on studying their reactivity and assessing their possible biological activities (4)(5)(6). Particularly in the pharmaceutical field, chalcones and their derivatives have been used in different applications for treating cancer (6)(7)(8)(9)(10)(11)(12).…”

“…Along with efficient methodologies implemented for their preparation, investigations have also focused on studying their reactivity and assessing their possible biological activities (4)(5)(6). Particularly in the pharmaceutical field, chalcones and their derivatives have been used in different applications for treating cancer (6)(7)(8)(9)(10)(11)(12).…”

“…Up to date, luminescent biomarker properties of chalcones were almost unknown. Therefore, to promote the use of chalcones as biomarkers in biological and medical fields, more studies concerning their cellular uptake, subcellular localization, and cytotoxicity (3,(6)(7)(8)10,11,14) should be undertaken.…”