DETERMINATION OF ADVANCED GLYCATION (AGEs) AND LIPOXIDATION (ALEs) END PRODUCTS IN FOODS AND BIOLOGICAL SYSTEMS: ADVANCES, CHALLENGES AND PERSPECTIVES

Barbosa, Júnia Helena Porto; Souza, Isis Tami de; Santana, Antônio Euzébio Goulart; Goulart, Marília Oliveira Fonseca

doi:10.5935/0100-4042.20160048

Cited by 4 publications

(4 citation statements)

References 64 publications

(99 reference statements)

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“…The enzyme catalyzed reactions have rate constants that are 100,000 times faster than non-enzymatic reactions and should be considered physiologically meaningful [29]. The qualitative formation of AGEs was verified by spectrofluorimetry measurements, with excitation and emission at wavelengths of 350 to 450 nm respectively [10,11].…”

Section: Introductionmentioning

confidence: 94%

“…Chemically AGEs are covalently linked (fluorescent and non-fluorescent) protein adducts, or cross-linking products between different amino acid residues. Several techniques can be used for the identification and quantification of AGEs, among which the following are notable: spectrofluorimetry, liquid and gas chromatography coupled to mass spectrometry and ELISA [10,11].…”

Section: Introductionmentioning

confidence: 99%

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New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System

Marques¹,

Trevisan²,

Maia³

et al. 2018

Preprint

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Advanced Glycation End Products (AGEs) represent a set of substances that contribute directly to the triggering and/or aggravation of pathologies associated with ageing. AGEs are produced by the reaction between reducing sugars (or α-dicarbonyl compounds) proteins and amino acid residues. Current methodologies require an incubation period of 1-3 weeks to generate AGEs. In this study the reaction time for the formation of AGEs (48 and 3 hours) is significantly reduced by coupling and adapting procedures already existing in the literature to the free radical generation system called the hypoxanthine/xanthine oxidase assay. The capacity of different classes and chemical compounds (aminoguanidine, chlorogenic acid, rutin, extracts of Hancornia speciosa Gomes) were evaluated to inhibit the protein glycation process, acting as capturing agents of α-dicarbonyl species. Aminoguanidine, rutin and the leaf extracts of Hancornia speciosa Gomes show a high capacity to act as α-dicarbonyl compound scavengers (RCS-trapping) and resulting in the inhibition of AGEs formation.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System

Marques¹,

Trevisan²,

Maia³

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…AGEs: advanced glycation end products formation of AGEs [17]. Table 9.1 shows the differences between food groups and cooking methods that contribute to low or high levels of AGEs [4,[18][19][20][21].…”

Section: Sources Of Agesmentioning

confidence: 99%

Dietary Advanced Glycation End Products as Mediators of Obesity: Cellular and Molecular Mechanisms of Action

Eleazu

Nna

Suleiman

et al. 2021

Cellular and Biochemical Mechanisms of Obesity

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Obesity, a disorder of body weight regulatory systems that is characterized by the accumulation of excess body fat is increasingly becoming a global pandemic. Despite several approaches that have been applied to mitigate obesity, they have not been able to totally reverse the obesity and its mediated complication. Advanced glycation end products (AGEs) refer to a group of prooxidant heterogenous compounds whose formation results from nonenzymatic reactions between reactive sugars and proteins, lipids and nucleic acids. While supporting evidence has suggested that AGEs may have contributory roles in the pathogenesis of obesity, there are indications that increased consumption of dietary AGEs increases the circulating AGEs levels and their deposition in the tissues including the adipose tissue, increasing the risk of development of obesity and its comorbidities. Identification of the underlying mechanism may provide an important strategy for novel therapeutic approaches against obesity. This chapter therefore provided novel insights into the role of dietary AGEs in the pathogenesis of obesity and the purported mechanisms of action.

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“… 2015 ; Barbosa et al. 2016 ). The adverse role of these AGE precursors is observed in a wide spectra of pathogenic conditions, including microvascular and macrovascular diseases such as nephropathy, retinopathy, peripheral neuropathy and arteriosclerosis in diabetes mellitus (Lo et al.…”

Section: Introductionmentioning

confidence: 99%

Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil

Xavier

Valentim

Camatari

et al. 2017

Pharmaceutical Biology

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Context: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil – sample A from Ribeira do Pombal and B, from Tucano – were investigated, with new information regarding their biological activities. Objective: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. Material and methods: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH•, FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0.04–25 μg/mL concentrations, for 72 h. The determination of primary phenols by ultra high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) and volatile organic compounds content by gas chromatography-mass spectrometry (GC-MS) were also performed. Results: The EEP polar fractions exhibited up to 90% protection against lipid peroxidation. The IC50 value for anti-glycation activity of EEP was between 16.5 and 19.2 μg/mL, close to aminoguanidine (IC50 = 7.7 μg/mL). The use of UHPLC-MS/MS and GC-MS allowed the identification of 12 bioactive phenols in the EEP and 24 volatile compounds, all already reported. Conclusions: The samples present good antioxidant/anti-glycation/cytotoxic activities and a plethora of biologically active compounds. These results suggest a potential role of propolis in targeting ageing and diseases associated with oxidative and carbonylic stress, aggregating value to them.

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DETERMINATION OF ADVANCED GLYCATION (AGEs) AND LIPOXIDATION (ALEs) END PRODUCTS IN FOODS AND BIOLOGICAL SYSTEMS: ADVANCES, CHALLENGES AND PERSPECTIVES

Cited by 4 publications

References 64 publications

New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System

New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System

Dietary Advanced Glycation End Products as Mediators of Obesity: Cellular and Molecular Mechanisms of Action

Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil

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