DERIVATIZATION AND SPECTROSCOPIC CHARACTERIZATION OF A BIOPOLYMER BASED ONL-LYSINE WITHD-BIOTIN ANALOGS:CO-POLY(L-LYSINE)-GRAFT-(E-N-[X-D-BIOTINYL]-L-LYSINE)

Martínez-Mancera, Flavio Dolores; Hernández-López, José Luis

doi:10.5935/0100-4042.20150173

Cited by 2 publications

(3 citation statements)

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“…43 Distinctively, holo-deoxyHb ZnPPIX embraced a one-power surface-controlled model as i p1,c scales up with υ (curve b) because ZnPPIX now plays as the built-in cofactor of a transmembrane-liked protein, and i p1,c of deoxyHb FePPIX also complied with this rule. Hence, the apparent coverage (Γ) can be reckoned that Γ-[deoxyHb ZnPPIX ] = 0.97 and Γ[deoxyHb FePPIX ] = 1.28 pmol/ mm 2 , 48 which is thought to be reasonable as Γ-[deoxyHb ZnPPIX ]/Γ[deoxyHb FePPIX ] = 75.8% close to the recombination rate (R = 83%). One step further, by means of the linear slope about E p1,c vs log(υ) and the Laviron equation, 49 the rate constant (k) of Fe(III) → Fe(II) could be extrapolated as 4.2 s −1 for oxyHb FePPIX and 3.8 s −1 of Zn(II) → Zn(I) for oxyHb ZnPPIX , which proves to be a competent manmade biocatalyst of comparable electroactivity.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Electrochemiluminescence-Repurposed Abiological Catalysts in Full Protein Tag for Ultrasensitive Immunoassay

et al. 2020

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Being announced as one of the “2019 Top Ten Emerging Technologies in Chemistry” by IUPAC, the directed evolution of artificial metalloenzymes has led to a broad scope of abiotic processes. Here, inspired by those key proteins in bioluminescence, a rudimentary expression of bio-electrochemiluminescent (ECL) macromolecules was achieved via the complexation of zinc proto-porphyrin IX (ZnPPIX) within apo-hemoglobin (apo-Hb). A high-yield monochromic irradiation at 644 nm could be provoked potentiostatically from the reconstituted holo-HbZnPPIX in solutions. Its secondary structure integrity was elucidated by UV and circular dichroism spectrometry, while voltammetry-hyphenated surface plasmon resonance authenticated its ligation conservativeness in electrical fields. Further conjugation with streptavidin rendered a homogeneous Janus fusion of both receptor and reporter domains, enabling a new abiological catalyst-linked ECL bioassay. On the other hand, singular ZnPPIX inside each tetrameric subunit of Hb accomplished an overall signal amplification without the bother of luminogenic heterojunctions. This pH-tolerant and non-photobleaching optics was essentialized to be the unique configuration interaction between Zn and O2, by which the direct electrochemistry of proteins catalyzed the transient progression of O2 → O2 ·– → O2* + hυ selectively. Such principle was implemented as a signal-on strategy for the determination of a characteristic cancer biomarker, the vascular endothelial growth factor, resulting in competent performance at a low detection limit of 0.6 pg·mL–1 and a wide calibration range along with good stability and reliability in real practices. This simple mutation repurposed the O2-transport Hb in the erythrocytes of almost all vertebrates into a cluster of oxidoreductases with intrinsic ECL activity, which would enrich the chromophore library. More importantly, its genetically engineered variants may come in handy in biomedical diagnosis and visual electrophysiology.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Electrochemiluminescence-Repurposed Abiological Catalysts in Full Protein Tag for Ultrasensitive Immunoassay

et al. 2020

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“…Another indication for the formation is the disappearing of the characteristic carbonylfinger band of the acid group at 1700 cm –1 . Furthermore, amide I bands were detectable at 1614 cm –1 . Rising of a new peak at 3338 cm –1 could not be associated to characteristic group signals according to literature.…”

Section: Resultsmentioning

confidence: 64%

“…Furthermore, amide I bands were detectable at 1614 cm −1 . 22 Rising of a new peak at 3338 cm −1 could not be associated to characteristic group signals according to literature.…”

Section: Synthesis and Characterization Of Polymersmentioning

confidence: 85%

N-Hydroxysulfosuccinimide Esters versus Thiomers: A Comparative Study Regarding Mucoadhesiveness

et al. 2019

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The objective of the study was to compare poly(acrylic acid)-N-hydroxysulfosuccinimide reactive esters (PAA-Sulfo-NHS) and poly(acrylic acid)-cysteine conjugates (PAA-Cys) regarding their mucoadhesiveness. Polymer conjugates were synthesized in a water free environment and characterized by UV–vis spectroscopy and FTIR. Water uptake studies were performed, and the polymers were further examined for their mucoadhesive properties and cohesiveness using the rotating cylinder method. Tensile force measurements were conducted to define the strength of adhesion to porcine intestinal mucosa. Additionally, polymer–mucus mixtures were assessed for rheological synergism by measuring the increase in dynamic viscosity. Both modifications led to a prolonged adhesion time compared to unmodified PAA. Fast dissolution of PAA-Sulfo-NHS derivatives was monitored, whereas PAA-Cys tended to extensively swell while exhibiting high cohesive properties. Measurements of tensile force revealed up to 2.7-fold (PAA-Sulfo-NHS) and 2.3-fold (PAA-Cys) enhancement of the maximum detachment force and 7.6-fold (PAA-Sulfo-NHS) and 3.6-fold (PAA-Cys) increase in the total work of adhesion. Formation of a gel network between polymer and mucus was confirmed by a 10.8-fold (PAA-Sulfo-NHS) and 20.8-fold (PAA-Cys) increase in viscosity. Both types of polymers show high mucoadhesive properties due to the formation of covalent bonds with the mucus. As thiolated polymers are capable of forming stabilizing disulfide bonds within their polymeric network, they are advantageous over PAA-Sulfo-NHS.

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DERIVATIZATION AND SPECTROSCOPIC CHARACTERIZATION OF A BIOPOLYMER BASED ONL-LYSINE WITHD-BIOTIN ANALOGS:CO-POLY(L-LYSINE)-GRAFT-(E-N-[X-D-BIOTINYL]-L-LYSINE)

Cited by 2 publications

References 1 publication

Electrochemiluminescence-Repurposed Abiological Catalysts in Full Protein Tag for Ultrasensitive Immunoassay

Electrochemiluminescence-Repurposed Abiological Catalysts in Full Protein Tag for Ultrasensitive Immunoassay

N-Hydroxysulfosuccinimide Esters versus Thiomers: A Comparative Study Regarding Mucoadhesiveness

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