Study of Immobilization of Lipase in Silica by the Sol-Gel Technique

Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free

“…The data implies that the immobilization procedure had the potential to increase the thermal stability. Similar results were reported by Ficanha et al, 15 that analyzed the thermal stability of the immobilized CALB on xerogels. Souza,19 has wrote that immobilized CALB enzyme onto magnetic nanoparticle lost its activity around 20% after 10 min at 60 ºC and the free enzyme, under the same conditions, lost more than 70% of its initial activity.…”

“…The results have demonstrated that the immobilized CALB on this support had a good reuse capacity, what make it useful to industrial application. Similar results were reported by Ficanha et al, 15 who have studied the residual activity of the immobilized CALB on xerogels and have obtained 9 reuses on the basic xerogels support. Macario et al 22 also have studied the immobilization of the Rhizomucor miehei (RML) lipase on different zeolitic supports and have obtained 3 reuse cycles.…”

“…The operational stability related to reuse was performed on a continuous way, following the methodology described by Ficanha et al 15 After reacting for 40 min, the reaction medium was removed from the system, remaining just the solid phase (enzyme/support complex), so the standard solution was re-added (oleic acid and ethanol) and the sample was submitted to a new reaction. When the enzyme/support complex has reached residual activity less or equal to 50% of the initial activity, the reactions stopped to be repeated.…”

“…The esterification activity was determined through the synthesis reaction of ethyl oleate, which was synthetized using oleic acid and ethanol at a molar ratio of 1:1 (standard solution), according to the method describe by Ficanha et al, 15 0.1 g of free or immobilized enzyme was added in 5 mL of standard solution. The reaction occurred at 40 °C in a closed flask using rotary shaker during 40 min.…”