State of the Art Two-Dimensional Liquid Chromatography: Fundamental Concepts, Instrumentation, and Applications

Mogollón, Noroska Gabriela Salazar; Lima, Paula Feliciano de; Gama, Mariana R.; Furlan, Mayra Fontes; Braga, Soraia Cristina Gonzaga Neves; Prata, Paloma Santana; Jardim, Isabel Cristina Sales Fontes; Augusto, Fábio

doi:10.5935/0100-4042.20140261

Cited by 4 publications

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“…The main sample preparation techniques that are easily on‐line coupled with a chromatographic instrument by utilizing the so‐called column switching approach to operate multidimensional separations are: On‐line SPE, in‐tube SPME, and turbulent flow chromatography . The instrumentation, set ups and characteristics of these sample preparation techniques will be discussed below.…”

Section: On‐line Sample Preparation Techniques Utilizing Column Switcmentioning

confidence: 99%

On‐line approaches for the determination of residues and contaminants in complex samples

Fumes

Andrade

Franco

et al. 2016

J of Separation Science

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The determination of residues and contaminants in complex matrices such as in the case of food, environmental, and biological samples requires a combination of several steps to succeed in the aimed goal. At least three independent steps are integrated to provide the best available situation to deal with such matrices: (1) a sample preparation technique is employed to isolate the target compounds from the rest of the matrix; (2) a chromatographic (second) step further "purifies" the isolated compounds from the co-extracted matrix interferences; (3) a spectroscopy-based device acts as chromatographic detector (ideally containing a tandem high-resolution mass analyzer) for the qualitative and quantitative analysis. These techniques can be operated in different modes including the off-line and the on-line modes. The present report focus the on-line coupling techniques aiming the determination of analytes present in complex matrices. The fundamentals of these approaches as well as the most common set ups are presented and discussed, as well as a review on the recent applications of these two approaches to the fields of bioanalytical, environmental, and food analysis are critically discussed.

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Section: On‐line Sample Preparation Techniques Utilizing Column Switcmentioning

confidence: 99%

On‐line approaches for the determination of residues and contaminants in complex samples

Fumes

Andrade

Franco

et al. 2016

J of Separation Science

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Fast determination of free metronidazole in rat plasma and peritoneal fluid using HPLC‐UV method

Michele

Bergoza

Gisele

et al. 2022

Biomedical Chromatography

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Peritonitis refers to the inflammatory reaction of the peritoneum to aggression. In addition, it contributes significantly to sepsis. The presence of free concentrations of antimicrobials above the minimum inhibitory concentration at the site of infection is critical to therapeutic response. Metronidazole (MTZ) is an antimicrobial used to treat peritonitis because of its effectiveness against anaerobic microorganisms. This study investigates free MTZ concentrations in peritoneal microdialysate in Wistar rats. A C 18 column (150 Â 4.0 mm, 5 μm) was used for the analysis conducted at 40 C under isocratic conditions. The mobile phase consisted of acetonitrile and an aqueous solution of 50-mM monobasic phosphate buffer and 0.1% triethylamine, with pH 3.0 (10:90, v/v). MTZ calibration was linear in the range of 0.5-30.0 μg/ml. The intra-and inter-day precision was satisfactory with relative standard deviation ≤5.67%. The accuracy ranged from 90.64 to 103.77%, and the lower limit of quantification was 0.5 μg/ml. The developed method was successfully applied in a pilot pharmacokinetic study after MTZ administration (30 mg/kg, intravenously) in rats. The main advantage of the employed method is that it does not require sample processing and protein removal steps. This is the first study to be conducted using MTZ in rats.

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Metabolomics Data Treatment: Basic Directions of the Full Process

Obando

Duarte

Simionato

2021

Advances in Experimental Medicine and Biology

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State of the Art Two-Dimensional Liquid Chromatography: Fundamental Concepts, Instrumentation, and Applications

Cited by 4 publications

References 0 publications

On‐line approaches for the determination of residues and contaminants in complex samples

On‐line approaches for the determination of residues and contaminants in complex samples

Fast determination of free metronidazole in rat plasma and peritoneal fluid using HPLC‐UV method

Metabolomics Data Treatment: Basic Directions of the Full Process

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