Optimization of Liquid-Liquid Extraction Step for Clavulanic Acid From Fermentation Broth Using Solvent Mixtures

Mancilha, Mariane de A.; Guimarães, Gabriela C.; Nardi, João Cláudio S. de C.; Oliveira, José Henrique M.; Hirata, Daniela B.

doi:10.5935/0100-4042.20140213

Cited by 1 publication

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“…Furthermore, organic solvent mixtures containing methyl‑ethyl ketone/ethyl acetate and methyl‑isobutyl ketone/ethyl acetate allow higher extraction levels of CA from fermentation broths. The equivolumetric mixtures of methyl‑ethyl ketone/ethyl acetate and ethyl‑isobutyl ketone/ethyl acetate led to extraction yields of 50 and 44.7%, respectively, which is approximately 30% higher compared to the obtained for single ethyl acetate extraction [ 200 ].…”

Section: Downstream Processing Of Camentioning

confidence: 99%

Clavulanic Acid Production by Streptomyces clavuligerus: Insights from Systems Biology, Strain Engineering, and Downstream Processing

2021

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Clavulanic acid (CA) is an irreversible β-lactamase enzyme inhibitor with a weak antibacterial activity produced by Streptomyces clavuligerus (S. clavuligerus). CA is typically co-formulated with broad-spectrum β‑lactam antibiotics such as amoxicillin, conferring them high potential to treat diseases caused by bacteria that possess β‑lactam resistance. The clinical importance of CA and the complexity of the production process motivate improvements from an interdisciplinary standpoint by integrating metabolic engineering strategies and knowledge on metabolic and regulatory events through systems biology and multi-omics approaches. In the large-scale bioprocessing, optimization of culture conditions, bioreactor design, agitation regime, as well as advances in CA separation and purification are required to improve the cost structure associated to CA production. This review presents the recent insights in CA production by S. clavuligerus, emphasizing on systems biology approaches, strain engineering, and downstream processing.

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Section: Downstream Processing Of Camentioning

confidence: 99%