CHEMICAL CONSTITUENTS ISOLATED FROMTurnera subulataSm. AND ELECTROCHEMICAL CHARACTERIZATION OF PHAEOPHYTIN B

Abstract

Turnera subulata Sm., known as "Chanana" or "flor-do-Guarujá" in Brazilian folklore, is a plant species belonging to the subfamily Turneroideae of family Passifloraceae, which is used for various medicinal purposes in Brazil. The phytochemical study conducted here led to the isolation and identification of ten compounds present in T. subulata: two mixtures of steroids, sitosterol and stigmasterol (nonglycosylated and glycosylated); a mixture of flavonoids, 5,7,4′-trihidroxiflavona-8-C-α-glucopyranoside and 5,7,3′,4′-tetrahidroxiflavona-8-C-α-glucopyranosidel; and four phaeophytins, phaeophytin purpurin-18-phytyl ester, a rare natural product, phaeophytin a, 13 2 -hydroxy-(13 2 -S)-phaeophytin a, and phaeophytin b. Phaeophytin b exhibited electrochemical activity similar to that of phthalocyanines.

Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from Suaeda vermiculata for Antioxidant and Cytotoxic Activities

“…The sephadex column also isolated pure steroidal compounds (β-sitosterol) in addition to a mixture of steroids that were eluted all together. The IR spectrum of pheo-a exhibited absorptions at 2922 (C-sp3), 3435 (NH), 1694 (CO) and 1377 cm −1 (CN); these results are consistent with the presence of a porphyrin nucleus in this compound [23]. The proton nuclear magnetic resonance ( 1 H-NMR) spectrum of pheo-a showed characteristic peaks for signals of the porphyrin skeleton and pheophytin a, as the spectrum appears at δ H 3.17, 3.40, and 3.67 ppm for three singlet methyl at position number 7, 2, and 12, respectively.…”