Immobilization of lignocellulolytic enzymes in magnetic nanoparticles

Sánchez-Ramírez, Jaquelina; Martínez-Hernández, José L.; Segura-Ceniceros, Elda Patricia; Contreras-Esquivel, Juan Carlos; Medina-Morales, Miguel A.; Aguilar, Cristóbal N.; Il’ina, A. M.

doi:10.5935/0100-4042.20140085

Cited by 7 publications

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis

Sánchez-Ramírez

Martínez-Hernández

Segura-Ceniceros

et al. 2016

Bioprocess Biosyst Eng

133

View full text Add to dashboard Cite

In the present study, Trichoderma reesei cellulase was covalently immobilized on chitosan-coated magnetic nanoparticles using glutaraldehyde as a coupling agent. The average diameter of magnetic nanoparticles before and after enzyme immobilization was about 8 and 10 nm, respectively. The immobilized enzyme retained about 37 % of its initial activity, and also showed better thermal and storage stability than free enzyme. Immobilized cellulase retained about 80 % of its activity after 15 cycles of carboxymethylcellulose hydrolysis and was easily separated with the application of an external magnetic field. However, in this reaction, K was increased eight times. The immobilized enzyme was able to hydrolyze lignocellulosic material from Agave atrovirens leaves with yield close to the amount detected with free enzyme and it was re-used in vegetal material conversion up to four cycles with 50 % of activity decrease. This provides an opportunity to reduce the enzyme consumption during lignocellulosic material saccharification for bioethanol production.

show abstract

Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis

Sánchez-Ramírez

Martínez-Hernández

Segura-Ceniceros

et al. 2016

Bioprocess Biosyst Eng

133

View full text Add to dashboard Cite

show abstract

Tyrosinase enzyme purification and immobilization from Pseudomonas sp. EG22 using cellulose coated magnetic nanoparticles: characterization and application in melanin production

El-Aziz,

Faraag,

Ibrahim

et al. 2023

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Melanin is a brown-black pigment with significant roles in various biological processes. The tyrosinase enzyme catalyzes the conversion of tyrosine to melanin and has promising uses in the pharmaceutical and biotechnology sectors. This research aims to purify and immobilize the tyrosinase enzyme from Pseudomonas sp.EG22 using cellulose-coated magnetic nanoparticles. Various techniques were utilized to examine the synthesized nanoparticles, which exhibited a spherical shape with an average diameter of 12 nm and a negative surface potential of − 55.7 mV with a polydispersity index (PDI) of 0.260. Comparing the immobilized magnetic tyrosinase enzyme with the free enzyme, the study’s findings showed that the immobilized tyrosinase enzyme had optimal activity at a pH of 6 and a temperature of 35 °C, and its activity increased as the concentration of tyrosine increased. The study investigated the antibacterial and anticancer bioactivity of the enzyme’s melanin product and found that it exhibited potential antibacterial activity against a multi-drug resistant strain including S. aureus and E. coli. The produced melanin also demonstrated the potential to decrease cell survival and induce apoptosis in initiation cells.

show abstract

Laccase Validation as Pretreatment of Agave Waste Prior to Saccharification: Free and Immobilized in Superparamagnetic Nanoparticles Enzyme Preparations

Sánchez-Ramírez

Martínez-Hernández

López-Campos³

et al. 2016

Waste Biomass Valor

View full text Add to dashboard Cite

Immobilization of lignocellulolytic enzymes in magnetic nanoparticles

Cited by 7 publications

References 1 publication

Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis

Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis

Tyrosinase enzyme purification and immobilization from Pseudomonas sp. EG22 using cellulose coated magnetic nanoparticles: characterization and application in melanin production

Laccase Validation as Pretreatment of Agave Waste Prior to Saccharification: Free and Immobilized in Superparamagnetic Nanoparticles Enzyme Preparations

Contact Info

Product

Resources

About