Human cornea conservation in coconut water solution

Cid, David Antonio Camelo; Araújo, Marília Cavalcante; Passos, Márcia Vitorino Sampaio; Brasil, Ivelise Regina Canito; Nunes, J. F.; Salgueiro, C. C. de M.; Nogueira, Sávio Carvalho; Costa, Delson João da

doi:10.5935/0004-2749.20210027

Cited by 2 publications

(4 citation statements)

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“…The main characteristics of the included studies, such as storage conditions, time and temperature ranges, the comparative distinct exposition media, and the assessment methods are shown in Table 4. Of the 14 included studies, 13 evaluated the effect of preservation in human corneas [5,12,[22][23][24][25][26][27][28][29][30][31][32]; 1 study was performed with rabbit cornea for a preclinical evaluation of new intermediate storage media with simple formulations [8].…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Optisol-GS was compared with seven distinct commercial media: Chen medium [31], Cornea Cold medium [13], Cornisol [22], Dexsol [25], Eusol-C [27], Kerasave [30,32], and Sinasol [26]. Optisol-GS was also compared with other compositions, such as a coconut water-based solution [23], Optisol-GS supplemented with antibiotics [24,28,29,33], and a minimum essential medium (MEM) with supplements [33].…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Regarding the corneal preservation, experimental times ranged from 1 to 28 days. The most investigated experimental times were 7 days [13,[20][21][22][23][24][25][26][28][29][30][31]34], and 14 days [13,22,24,26,28,30,34]. The mainly reported hypothermic temperature was 4 • C [8,[24][25][26][27]29,[31][32][33] (Table 4).…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Endothelial cell morphology was assessed in eight studies [13,23,25,26,[29][30][31] by the methods of slit-lamp biomicroscopy [23,26,31], specular microscopy [26], and electron microscopy [20,25,26,31] (Table 10). Three studies assessed the corneal transparency [13,23,30] (Table 10). The complete data characterizing the studies are provided in Tables 4, 9 and 10.…”

Section: Cornea Preservation Qualitative Parametersmentioning

confidence: 99%

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Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review

et al. 2022

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Optisol-GS is the most widely used pharmaceutical composition to preserve corneas for transplantation. This systematic review investigated the effects of different cold corneal storage media (CCSM) compared with Optisol-GS on the quality of stored corneas. The literature was searched throughout May 2022 on six databases and grey literature. Studies including corneas (population) exposed to distinct cold storage media (exposure) and Optisol-GS (comparison) that reported qualitative and/or quantitative parameters of cornea quality (outcome) were included. Methodological quality was assessed using ToxRTool. From 4520 identified studies, fourteen were included according to the eligibility criteria, comprising 769 evaluated cornea samples comparing Optisol-GS with commercial and noncommercial media. All studies showed good methodological quality. Experimental times ranged from 1–28 days, mainly using 4 °C as the preservation temperature. Viable endothelial cell density (ECD) and endothelial cell morphology (EC) were the most assessed parameters. ECD results for Cornisol were higher than Optisol-GS in 10 days (p = 0.049) and favored Cornea ColdTM up to 4 weeks (p < 0.05), which also showed better qualitative results. While the standardization of test protocols could improve comparisons, evidence indicates that most CCSM present similar performances on cornea preservation for transplantation at seven days, while some formulations may increase preservation at extended times.

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Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Cornea Preservation Qualitative Parametersmentioning

confidence: 99%

See 2 more Smart Citations

Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review

et al. 2022

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Swept-source OCT for corneal graft quantitative evaluation in the eye bank and the correlation of the measurements to pre-excision values

Kałużny

Mlyniuk

Liberski

et al. 2022

Sci Rep

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Quantitative evaluation of the human corneal grafts stored in the tissue banks is usually limited to endothelial cell density and central thickness. Swept-source OCT (SS-OCT) is capable of measuring the central curvatures of the corneal tissue prepared for transplantation without loss of sterileness, providing insights on its refractive state. The aim of the paper is to compare in vitro SS-OCT measurements with pre-excision values. Hand-held keratometry and ultrasound pachymetry was performed on 22 corneas before excision of corneoscleral button and insertion in the vial with Eusol-C solution (AlchimiaS.r.l, Nicolò, Italy). After 12 to 36 h of hypothermic storage the corneas were examined within the vials with custom built SS-OCT system maintaining a sterile environment. The anterior and posterior central curvatures, and central corneal thickness (CCT) were measured. Rotation of the corneoscleral button was controlled by making a 6-o'clock mark during excision. Mean pre-excision CCT was 626.45 ± 28.71 µm and 468.05 ± 52.96 µm when measured with SS OCT (r = 0.55; p < 0.001). Respective values for average keratometry were 7.74 ± 0.39 mm and 7.92 ± 0.57 mm (r = 0.6; p = 0.22). Although high differences were observed in corneal thickness, keratometric radius of curvature at the flat (r = 0.42; p < 0.001) and steep (r = 0.62; p = 0.014) meridian of the anterior corneal surface, as well as corneal anterior astigmatism (r = 0.3; p < 0.001), showed good correlation with pre-excision values. SS-OCT is capable of providing quantitative evaluation of the human corneal grafts in hypothermic storage. Good correlation between curvature measurements before excision and during banking in the vial indicates its clinical utility.

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Human cornea conservation in coconut water solution

Cited by 2 publications

References 0 publications

Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review

Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review

Swept-source OCT for corneal graft quantitative evaluation in the eye bank and the correlation of the measurements to pre-excision values

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