The influence of storage method on the transparency of pig crystalline lens

Artigas, Cristina; Fandiño, Adriana; Navea, Amparo; López-Murcia, María M; Bosch‐Morell, Francisco; Araújo-Miranda, R.; Artigas, José María Gatell

doi:10.5935/0004-2749.20190011

Cited by 2 publications

(1 citation statement)

References 14 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is important to note that, samples might be more transparent than what we have analyzed. Previous studies have shown that lenses stored in formalin lose nearly 30% of their transparency [ 13 ]. However, since all our samples were processed the same, relative transparency between samples is maintained and this is what we compared.…”

Section: Discussionmentioning

confidence: 99%

Soemmerring’s Rings Developed around IOLs, in Human Donor Eyes, Can Present Internal Transparent Areas

D’Antin

Tresserra

Barraquer

et al. 2022

IJMS

View full text Add to dashboard Cite

Soemmerring’s rings consist of a ring of lens epithelial derived cells that grow along the periphery of an aphakic lens capsule, or around an intraocular lens. These rings when visualized frontally, appear opaque, however, in some cases the cells that compose these rings are organized in the same fashion as those in normal transparent adult lenses. Thus, our purpose was to test whether any part of the adult Soemmerring’s ring could be transparent and how this related to morphological factors. To study this, 16 Soemmerring’s rings were extracted from donor eye globes. After imaging, they were thickly sectioned sagittally in order to analyze the degrees of transparency of different areas. All samples were also histologically analyzed using alpha smooth muscle actin, Vimentin, wheat germ agglutinin and DAPI. Our results showed that many samples had some transparent areas, mostly towards the center of their cross-section. Of the factors that we analyzed, only lens fiber organization at the bow region and an increased area of mature lens fiber cells had a significant relation to the degree of transparency at the center. Thus, we can conclude that as Soemmerring’s rings mature, they can develop organized and transparent areas of lens cells.

show abstract

Section: Discussionmentioning

confidence: 99%