Microbiological analysis of contact lens cases: impact of the hospital
            environment

Bôas, Vinicius Targa Villas; Júnior, Gildásio Castello de Almeida; Almeida, Margarete Teresa Gottardo de; Gonçalves, Maicon Santos; Coelho, Leandro Fernandes

doi:10.5935/0004-2749.20180074

Cited by 8 publications

(7 citation statements)

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“…Many variables were present in the study not allowing the exact type of bacteria to be identified. But more than 85% of the bacteria were gram-negative bacilli [36].…”

Section: Discussionmentioning

confidence: 99%

Microbial Analysis of Contact Lenses

Tamilmani

Muralidharan

2021

JPRI

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Introduction: Contact lenses are small, thin lenses which are worn directly on the surface of the eyes. They can be worn aesthetically or to correct vision. Contact lens related eye infections can lead to serious complications such as blindness, and are associated with several risk factors such as sleeping with lenses, exposure to water, not adhering to replacement schedules, and reusing disinfecting solutions, among others. The severity of the infection may vary with the degree of pathogenicity of the microorganism. Hygiene and handling of contact lenses play a very important role. The main aim of this study is to assess the microbial analysis of contact lenses. Materials and Methods: A total of 15 lenses were collected in 2 ml sterile saline solution individually and manually agitated for five minutes. The lens was then removed using a sterile toothpick from the container. The sterile container was stored at 4°C till it was processed. 50 microliter of the sample was transferred using a pipette and inoculated on nutrient agar, blood agar and sabouraud dextrose agar. The microorganism (fungus or bacteria) were identified by standard protocol. Results and Discussion: Bacillus, Staphylococcus aureus and CONS [Coagulase Negative Staphylococci] were found in blood agar. No fungal growth was found among the samples. More importance could be given to contact lenses handling and hygiene to avoid eye related bacterial and fungal infections. Conclusion: In the present study, the total CFU (Colony Forming Unit) was found to be confluent in all the participants who wore contact lenses for 4 months compared to those who have worn contact lenses from 15 and 28 days. Only bacterial growth was seen in the culture plate. There was no fungal growth seen from the samples collected.

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“…Many variables were present in the study not allowing the exact type of bacteria to be identified. But more than 85% of the bacteria were gram-negative bacilli [36].…”

Section: Discussionmentioning

confidence: 99%

Microbial Analysis of Contact Lenses

Tamilmani

Muralidharan

2021

JPRI

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“…This finding could be owing to the higher rate of contact lens use (40.4%), which is a risk factor for gram-negative infections. 13,17…”

Section: Discussionmentioning

confidence: 99%

Microorganisms and Antibiotic Resistance of Bacterial Keratitis at a Rural County Hospital in Seville

Pascua¹,

Bernal²,

Sanchez³

et al. 2022

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

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To review the incidence, resistance patterns, and management of bacterial keratitis during the past 4 years. Methods: We retrospectively reviewed the clinical records of microbiological isolates from patients with a clinical diagnosis of bacterial keratitis. Results: A total of 159 patients were analyzed, and 102 microorganisms were isolated from 129 cultures. In these cultures, 23.7% of the microorganisms were gram positive, 60.8% were gram negative, and 15.5% were fungi. Pseudomonas aeruginosa was the most common bacteria (9.2%), followed by Serratia marcescens (4.4%) and Staphylococcus aureus (4%). Resistance to fluoroquinolones and aminoglycosides was found to be 23.1% and 53.1% in gram-positive and 2.8% and 13.9% in gram-negative bacteria, respectively. Resistance to ceftazidime against gram-negative bacteria was 13.9%. No resistance to vancomycin was observed. Conclusions: A high resistance rate to aminoglycosides and fluoroquinolones was observed in gram-positive bacteria. We concluded that fluoroquinolones or aminoglycosides may not be suitable for initial monotherapy in patients with severe bacterial keratitis.

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“…Guidance from professional bodies and solution manufacturers with respect to lens case maintenance may be contradictory [467]; lens case replacement advice varies between 1-3 months and few mention the need to rub and store cases face down. Further efforts to minimise case contamination may be achieved by use of non-ridged cases [468] and possibly cylindrical cases [469]. Promising outcomes in limiting biofilm formation have also been reported, through incorporation of various compounds into the materials of polypropylene contact lens cases [470][471][472], but additional testing is needed to establish their biocompatibility and safety.…”

Section: Cleaning Instructionmentioning

confidence: 99%