Oxidative stress of crystalline lens in rat menopausal model

Abstract

Purpose: To evaluate lenticular oxidative stress in rat menopausal models. Methods: Forty Wistar female albino rats were included in this study. A total of thirty rats underwent oophorectomy to generate a menopausal model. Ten rats that did not undergo oophorectomy formed the control group (Group 1). From the rats that underwent oophorectomy, 10 formed the menopause control group (Group 2), 10 were administered a daily injection of methylprednisolone until the end of the study (Group 3), and the remaining 10 rats were administered intraperitoneal streptozocin to induce diabetes mellitus (Group 4). Total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) measurements of the crystalline lenses were analyzed. Results: The mean OSI was the lowest in group 1 and highest in group 4. Nevertheless, the difference between the groups was not statistically significant in terms of OSI (p>0.05). The mean TOS values were similar between the groups (p>0.05), whereas the mean TAC of group 1 was significantly higher than that of the other groups (p<0.001). Conclusions:Our results indicate that menopause may not promote cataract formation.

Rosmarinic and Sinapic Acids May Increase the Content of Reduced Glutathione in the Lenses of Estrogen-Deficient Rats

“…A decreased GSH content in the lenses is well documented in various rat cataract models [48,49,50,51,52]. However, there are only few reports describing the GSH content or antioxidative abilities in the lenses of the laboratory animals with estrogen deficiency [53,54]. A study conducted on ovariectomized mice showed that GSH content in the lenses did not change when compared with control animals [53].…”

“…A study conducted on ovariectomized mice showed that GSH content in the lenses did not change when compared with control animals [53]. Acer et al [54] examined total non-enzymatic antioxidant content in the lenses of ovariectomized rats conducting total antioxidant capacity (TAC) test and noted that TAC in the lenses of estrogen-deficient rats was significantly lower than in the lenses of control rats [54]. The results for GSH content in the lenses obtained in our study overlap with these for TAC presented by Acer et al [54], possibly due to the fact that GSH is a predominant non-enzymatic antioxidant in the lenses [47].…”

miR-24-p53 pathway evoked by oxidative stress promotes lens epithelial cell apoptosis in age-related cataracts

“…It has been widely reported that the lens is subjected to oxidative stress throughout its life and that oxidative damage is a major cause of cataract formation ( 21 ). Current research also suggests that with increasing age, the accumulation of oxidized lens components and decreased capacity of repair mechanisms result in increased levels of reactive oxidative species ( 22 ) and LEC death, promoting cataractogenesis ( 21 , 23 ). In the present study, we used H 2 O 2 to induce LEC death and apoptosis as an oxidative stress model.…”

Wpływ naryngeniny na odpowiedź antyoksydacyjną oraz status oksydacyjny w soczewkach szczurów z cukrzycą

“…Zmiany te świadczą o rozwoju stresu oksydacyjnego w soczewkach szczurów z cukrzycą. Stres oksydacyjny w soczewkach szczurów, opisany takimi samymi parametrami, został zaobserwowany również w modelu szczurów z niedoborem estrogenów oraz hiperglikemią [36] oraz po ekspozycji szczurów na promieniowanie UVC [37]. Zaobserwowano również, iż indukowanie cukrzycy spowodowało istotne obniżenie zawartości zarówno białkowych, jak i niebiałkowych grup tiolowych w soczewkach badanych zwierząt.…”