Impact of iron overload on interleukin-10 levels, biochemical parameters and oxidative stress in patients with sickle cell anemia

Abstract

ObjectiveThe aim of this study was to evaluate the impact of iron overload on the profile of interleukin-10 levels, biochemical parameters and oxidative stress in sickle cell anemia patients. MethodsA cross-sectional study was performed of 30 patients with molecular diagnosis of sickle cell anemia. Patients were stratified into two groups, according to the presence of iron overload: Iron overload (n = 15) and Non-iron overload (n = 15). Biochemical analyses were performed utilizing the Wiener CM 200 automatic analyzer. The interleukin-10 level was measured by capture ELISA using the BD OptEIAT commercial kit. Oxidative stress parameters were determined by spectrophotometry. Statistical analysis was performed using GraphPad Prism software (version 5.0) and statistical significance was established for p-values < 0.05 in all analyses. ResultsBiochemical analysis revealed significant elevations in the levels of uric acid, triglycerides, very low-density lipoprotein (VLDL), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), urea and creatinine in the Iron overload Group compared to the Non-iron overload Group and significant decreases in the high-density lipoprotein (HDL) and low-density lipoprotein (LDL). Ferritin levels correlated positively with uric acid concentrations (p-value < 0.05). The Iron overload Group showed lower interleukin-10 levels and catalase activity and higher nitrite and malondialdehyde levels compared with the Non-iron overload Group. ConclusionThe results of this study are important to develop further consistent studies that evaluate the effect of iron overload on the inflammatory profile and oxidative stress of patients with sickle cell anemia.

Serum Ferritin Is Inversely Correlated with Testosterone in Boys and Young Male Adolescents: A Cross-Sectional Study in Taiwan

“…A human study reported that IL-10 supplementation is associated with increased risks of hyperferritinemia and anemia in Crohn’s disease patients [ 27 ]. On the other hand, sickle cell anemia patients with iron overload, defined by elevated serum ferritin of >2247 pmol/L, had lower serum IL-10 levels compared to non-iron-overloaded patients [ 28 ]. Future studies investigating the interactive effects of IL-10 and serum ferritin on testosterone are needed in order to understand how a shift in the anti-/proinflammatory balance contributes to testosterone levels in boys and adult men.…”

Serum hepcidin levels in women infected with HIV-1 under antiviral therapy

“…A previous study has demonstrated that, in rats receiving toxic doses of iron, the elevation of hepcidin expression in the liver was accompanied concomitantly by a significant increase of serum uric acid levels [Toledano et al, ]. It has been shown that in patients with iron overload, urea and ferritin, a protein that stores iron in the cell, levels correlated positively with uric acid levels [Barbosa et al, ]. Although iron is essential for human life, free iron in excess can have deleterious effects by catalyzing the Fenton reaction (ferrous ion‐mediated decomposition of hydrogen peroxide) and lipid peroxidation.…”

Cytokine profiles in sickle cell anemia: Pathways to be unraveled

“…IL-10 is an anti-inflammatory cytokine whose main effect is inhibition of the synthesis of various cytokines, such as TNF-α, GM-CSF, IL-1, IL-6, IL-8 and IL-12, to promote the uptake and retention of iron within monocytes and the reticuloendothelial system. IL-10 also inhibits the proliferation of TH1 cells, decreasing cytolytic function and the secretion of TH1 cytokines and facilitating the development of a TH2 response [57].…”