Comparing electrophoresis at alkaline pH and high performance liquid chromatography to diagnose Hb S-like hemoglobin

α-chain hemoglobin variants with electrophoretic mobility similar to that of hemoglobin S in newborn screening programs

“…The former include Hbs D, Lepore, Korle-Bu, Osu-Christiansborg and Zürich (13,14) , and the latter Hbs Hasharon, Stanleyville-II, G-Pest, Sunshine Seth, G-Philadelphia, West One and Daneshgah-Tehran (8,9,14) . In the case of the α-chain variants, some technical characteristics can help with the diagnosis; these include the presence of four bands in electrophoresis - two corresponding to Hb A 2 (A 2 and A 2 ') and two to Hb A (A and 'X') - and a lower concentration of 'Hb X' than normally observed in individuals with β-chain variants.…”