Ultra-performance liquid chromatography coupled to electrospray ionization-tandem mass spectrometric method for simultaneous determination of acemetacin in presence of its metabolite indomethacin and degradation products

Background Green chemistry approach was developed for the purpose of saving the environment by using green solvents. Applying the green analytical chemistry principles for traditional methods is considered a challenge. Acemetacin is a commonly used analgesic prodrug that bioactivated to indomethacin. Objectives Developing two simple Eco-friendly chromatographic methods for simultaneous determination of acemetacin and indomethacin. Methods The first is HPLC-DAD method, separation was performed on Waters XBridge® Shield RP18 (250 × 4.6 mm, 5 µm) analytical column with ethanol–ammonium acetate buffer (50 mM, pH 3.5 ± 0.1) (60:40, v/v) as a mobile phase at a flow rate of 1 mL/min at 25 ± 0.5 °C and UV detection at 254 nm. The other method is TLC method coupled with densitometric quantification using pre-coated silica TLC-plates and butanol–ethyl acetate (70:30, v/v) elution system. The plates were scanned at 254 nm. Results Both methods were validated according to the ICH guidelines, linearity was confirmed, for both, over a concentration range of 1–100 µg/mL for the HPLC method and 0.2–7 µg/band for TLC-densitometric one. Methods’ greenness was evaluated by the National Environmental Methods Index, Eco-Scale, Green Analytical Procedure Index metrics and Analytical GREEnness Metric Approach. Conclusion The proposed methods were found to be suitable for determination of studied drugs in their marketed formulations and suggested for routine analysis in quality control laboratories. Highlights HPLC and TLC methods were validated for the assay of acemetacin and indomethacin. Methods’ greenness was evaluated by NEMI, Eco-Scale, GAPI and AGREE metrics and were compared to published methods. The developed HPLC method shortened the elution time of the analyzed drugs saving more time and money and the TLC method lowered the drugs’ detection limit.

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Ultra-performance liquid chromatography coupled to electrospray ionization-tandem mass spectrometric method for simultaneous determination of acemetacin in presence of its metabolite indomethacin and degradation products

Abstract: Ultra-performance liquid chromatography coupled to electrospray ionizationtandem mass spectrometric method for simultaneous determination of acemetacin in presence of its metabolite indomethacin and degradation products

Cited by 2 publications

References 7 publications

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Detection, transformation, and toxicity of indole-derivative nonsteroidal anti-inflammatory drugs during chlorine disinfection

Eco-Friendly Chromatographic Methods for Determination of Acemetacin and Indomethacin; Greenness Profile Assessment

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