Determination of dimenhydrinate and cinnarizine in combined dosage form in presence of cinnarizine impurity

Abstract

Three accurate, sensitive and time saving spectrophotometric methods have been developed and validated for determination of mixture of dimenhydrinate (DMH) and cinnarizine (CIN) in presence of cinnarizine impurity (1-(diphenylmethyl)piperazine) (IMP). In method A; dimenhydrinate was determined by measuring 1 D amplitudes at 292.0 nm while cinnarizine and its impurity were determined by 1 DD method at 256.2 and 219.6 nm, respectively, using standard spectrum of 20 µg/mL of dimenhydrinate as a divisor. Method B depends on dividing spectrum of ternary mixture by standard spectrum of 20 µg/mL of dimenhydrinate and then cinnarizine and its impurity were determined in the obtained ratio spectrum by ratio difference method using the difference between 219.0 and 237.2 nm and between 230.0 and 264.0 nm, respectively. On the other hand dimenhydrinate could be determined by dividing spectrum of ternary mixture by standard spectrum of 20 µg/mL of cinnarizine and then it were determined at the obtained ratio spectrum by ratio difference method using the difference between 216.8 and 232.8 nm. Method C is the mean cantering of ratio spectra method (MCR) where the amplitudes at 234.8, 240.0 and 233.6 nm in the second mean centering ratio spectra were used for determination of dimenhydrinate, cinnarizine and its impurity, respectively. The developed methods were validated according to ICH guidelines regarding good accuracy and precision, and they were successfully applied to pharmaceutical formulation and laboratory prepared mixtures. The results were statistically compared with those obtained by reported method and no significant difference was found. KEYWORDS

Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance

“…Determination of drugs in presence of their degradation products and impurities recently became an important point of interest for analysts especially if the drug degradations or impurities have hazardous effects [20][21][22][23][24][25]. Reviewing the published chromatographic methods for determination of LEV, acetonitrile was the organic modifier of choice and C18 column was the used stationary phase.…”

Experimentally designed chromatographic method for the simultaneous analysis of dimenhydrinate, cinnarizine and their toxic impurities

“…Moreover, CIN and DMH were simultaneously determined by TLC-densitometry, 29,30 HPLC 30,31 and UV-spectrophotometry. 32,33 The binary mixture of DMH and CIN was analyzes in presence of DPP a spectrophotometric, 34 TLC-densitometric 35 and an HPLC 35 methods. A single TLC-densitometric method was developed in our laboratory for the simultaneous assay of DMH, CIN and their toxic impurities; BZP and DPP.…”

Validated Spectrophotometric Methods for the Estimation of Cinnarizine in Binary Mixture With Paracetamol in Bulk and Tablets

“…Literature review revealed that several analytical methods were reported for the spectrophotometric determination of CIN in pharmaceutical preparations and biological fluids. These methods include spectrophotometric [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], and spectroflorometric [20][21][22], voltammetry [23], high-performance liquid chromatography (HPLC) [9,[24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41], high-performance thin-layer chromatography (HPTLC) [9,[41][42][43][44][45], and capillary electrophoresis [46]. Other methods were developed to determination of CIN in combination with PCM which include spectrophotometry [47], high-performance liquid chromatography (HPLC) and high-performance thin layer chromatography, (HPTLC)...…”