Application of π receptors to the spectrophotometric determination of naftidrofuryl oxalate in pure form and its pharmaceutical preparation

Abstract

KEYWORDSThree different spectrophotometric methods are developed for the determination of naftidrofuryl oxalate (NAF) in pure form and its pharmaceutical preparation. The methods are based on charge transfer complexation reactions of NAF as n-electron donor with either p-chloranilic acid (PCA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) or tetracyano ethylene (TCNE) as π-receptors to give highly colored anion radicals species. The colored products were quantified spectrophotometrically at 515, 588 and 396 nm in PCA, DDQ and TCNE methods, respectively. Under the optimized experimental conditions, Beer's law is obeyed over the concentration ranges of 75.0-300.0, 25.0-150.0 and 15.0-50.0 μg/mL NAF for PCA, DDQ and TCNE methods, respectively. The proposed methods were applied successfully to the determination of NAF in pure form and its commercial tablets with good accuracy and precision. Statistical comparison of the results was performed using Student's t-test and Fratio at 95% confidence level, showing that there is no significant difference between the reference and the proposed methods with regard to accuracy and precision. Further, the validity of the proposed methods was confirmed by standard addition technique.Chloranilic acid Tetracyanoethylene Naftidrofuryl oxalate Pharmaceutical preparation Charge transfer complexation 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

“…The proposed HPLC-UV method is very simple and rapid as it does not require any reagent or sample preparation. Furthermore in the previously reported HPLC methods [33][34][35][36], the mobile phase consisted of either complicated buffers which cause column corrosion, or methanol which has several disadvantages such as forming a relatively viscous solution with water giving high pressures, lower elution strength than acetonitrile, and producing a high noise in UV detection region. In addition, acute or chronic exposure to methanol by inhalation or ingestion has several health hazard effects such as blurred vision, headache, dizziness, and nausea.…”

“…In addition, the obtained results were statistically compared with those of reported method [36], in respect to accuracy and precision. At 95% confidence level, no significant difference was observed between the calculated and theoretical values of both the proposed and reported method using t-and F-tests as shown in Table 8.…”

“…A statistical comparison of the results obtained by analysis of pure rutin and ascorbic acid by the proposed and reported method [36] is shown in Table 6. The calculated t and F values are less than tabulated ones, which reveals that there is no significant difference with respect to accuracy and precision between the proposed and reference method.…”

“…In addition, Several methods have been reported for simultaneous determination of mixture of these active ingredients like UV spectrophotometry [23,24], electrochemical method [25][26][27], chemiluminescence [28], capillary electrophoresis [29][30][31][32], high-performance liquid chromatography (HPLC) [33][34][35][36]and NIR FTIR [37]. The objective of this study is to develop a simple, rapid, sensitive and selective method for determination of rutin and ascorbic acid mixture in their pure forms and pharmaceutical dosage forms.…”

Application of π acceptors to the spectrophotometric and spectrofluorimetric determination of vincamine and naftidrofuryl oxalate in their pharmaceutical preparations

“…Several methods have been reported for the determination of NF, including: titrimetry, HPLC, spectrophotometry, spectrofluorimetry and voltammetry…”