Fabrication and evaluation of potentiometric sensors of an anticancer drug (Gemcitabine)

Abstract

Accurate, rapid and inexpensive determination of gemcitabine, an anticancer drug, is of high interest. This manuscript describes the use of potentiometric sensors as a basis for this work given their known attractive characteristics that meet our needs. Potentiometric sensors were comprised of carbon paste S1, coated wire S2 and PVC membrane S3, of gemcitabine (an anticancer drug) were fabricated, studied and evaluated. The calibration plots for these electrodes showed a Nernstian slope of 58.4±0.3, 59.5±0.3 and 58.3±0.3 mV per decade with the limit of detection: 6.50×10-5, 7.20×10-5 and 4.60×10-5 for sensors S1, S2 and S3, respectively. The electrodes have a short and stable response time of ~5 seconds and good reproducibility in a pH range of 2.5-9.5. The present sensors show distinct selectivity toward the drug ion in comparison to several inorganic ions, sugars, amino acids and some common drug excipients. Gemcitabine was determined successfully in ampoules and urine using these sensors by the calibration curve method.

Application of Experimental Design Approaches and in Silico Molecular Docking on the Host-Guest Complexes with Cyclodextrin for the Analysis of Benazepril Hydrochloride in Pharmaceutical Formulation

“…19,20 Ion-selective electrodes are portable in nature enabling easier design and assembly, have faster response time, and are more selective. [21][22][23][24] However, many studies showed that using ion-selective sensors based on ionophores had significantly improved the selectively, response, and detection limit of electrodes compared to ion-pair-based sensors. 25,26 Various studies were declared on the progress and application of potentiometric ion-selective electrodes to estimate different drugs using cyclodextrins (CD ) diversity as a modifier relied on host-guest inclusion mechanism.…”