Exames parasitológicos, imunoistoquímicos e histopatológicos para detecÇão de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral

Abstract

ResumoO propósito do presente trabalho foi o estudo da Leishmaniose Visceral Canina -LVC por meio de métodos parasitológicos e imunoistoquímicos para a detecção de formas amastigotas de Leishmania (L.) chagasi em baço, além de descrever a histopatologia das lesões esplênicas em 34 cães, com diferentes manifestações clínicas da LVC, eutanasiados pelo Centro de Controle de Zoonoses de Ilha Solteira, SP. Esses animais foram examinados clinicamente antes da eutánásia e de acordo com os sinais clínicos da LVC, foram classificados em três grupos: assintomáticos (8 cães), oligossintomáticos (17 cães) e sintomáticos (9 cães). Após a realização desses exames, dos 34 cães, 22 (64,7%) estavam positivos e 12 (35,3%) negativos. Desses cães positivos, 1/22 (4,5%) era assintomático, 12/22 (54,5%) eram oligossintomáticos e 9/22 (40,1%) sintomáticos. Pela histopatologia, os cães, especialmente os sintomáticos apresentavam o baço com inflamação crônica e espessamento na região capsular e trabecular, além de extensa alteração morfológica na polpa vermelha e branca pela presença de grande quantidade de macrófagos repletos de amastigotas, pela reação granulomatosa inflamatória e pelas áreas hemorrágicas. Os exames histopatológicos e a detecção microscópica direta da L. (L.) chagasi revelaram que o baço é um órgão útil para auxiliar no diagnóstico da LVC. A coloração imunoistoquímica foi a que detectou o maior número de tecidos esplênicos positivos com amastigotas, além de elucidar os casos suspeitos pelos exames parasitológicos, principalmente, nos animais assintomáticos ou oligossintomáticos.Palavras-chave: Leishmania (L.) chagasi, Leishmaniose Visceral, cães, baço, imunoistoquímica. AbstractThe purpose of this work was a Canine Visceral Leishmaniasis -CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. The data of this work from histopathologic examination and direct microcopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest num...

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

“…Our results coincided with others studies (GIUNCHETTI et al, 2008;LIMA et al, 2007;TAFURI et al, 1996), who observed well-organized intralobular granulomas in the livers of infected dogs. The disorganized tissue architecture in the spleen relating to predominance of parasitized macrophages was observed in our study and others researches (SANTANA et al, 2008;TASCA et al, 2009).…”

Histological changes and immunolabeling ofLeishmania infantum in kidneys and urinary bladder of dogs

“…The glomerulonephritis pattern was membranoproliferative, which is in agreement with previously described findings in dogs with CVL (COSTA et al, 2003). The results from the present study confirm that immunohistochemistry is an important procedure for detect L. infantum in different organs (COSTA et al, 2003;TASCA et al, 2009). In addition, it is the first description of the presence of amastigote forms of L. infantum in the bladders of dogs.…”

Molecular and serological detection of Leishmania spp. in captive wild animals from Ilha Solteira, SP, Brazil

“…using serological and molecular methods in captive wild animals from a zoo located in Ilha Solteira, São Paulo, Brazil, an area where VL is endemic and affects about 9% of the 3,798 domestic canid population (ASSIS et al, 2008;TASCA et al, 2009;QUEIROZ et al, 2010).…”