Study of advanced rheumatoid arthritis

Carvalho, Carolina da Silva; Andrade, Luiz Eduardo Coelho; Keusseyan, Silene Peres; Rangel, João Lucas; Ferreira‐Strixino, Juliana; Martin, Aírton Abrahão; Raniero, Leandro

doi:10.4322/rbeb.2014.004

Cited by 15 publications

(13 citation statements)

References 73 publications

(75 reference statements)

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“…Rheumatoid arthritis (RA) is one of the most prevalent autoimmune and degenerative joint diseases with immune hyperactivation and synovitis ( 1 , 2 ). Non-steroidal anti-inflammatory drugs (NSAIDs), disease-modifying antirheumatic drugs (DMARDs), and glucocorticoids are the most commonly used drugs for the clinical treatment of RA ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

DC32, a Dihydroartemisinin Derivative, Ameliorates Collagen-Induced Arthritis Through an Nrf2-p62-Keap1 Feedback Loop

Fan

Yao

et al. 2018

Front. Immunol.

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Artemisinins have been reported to have diverse functions, such as antimalaria, anticancer, anti-inflammation, and immunoregulation activities. DC32 [(9α,12α-dihydroartemisinyl) bis(2′-chlorocinnmate)], a dihydroartemisinin derivative possessing potent immunosuppressive properties, was synthesized in our previous study. Collagen-induced arthritis (CIA) in DBA/1 mice and inflammatory model in NIH-3T3 cells were established to evaluate the effect of DC32 on RA and discover the underlying mechanisms. The results showed that DC32 could markedly alleviate footpad inflammation, reduce cartilage degradation, activate the Nrf2/HO-1 signaling pathway, and increase the transcription of p62 in DBA/1 mice with CIA. Further mechanistic exploration with NIH-3T3 cells indicated that DC32 could increase the transcription, expression, and nuclear translocation of Nrf2. In addition, DC32 promoted degradation of Keap1 protein and upregulated HO-1 and p62 expression. Furthermore, the effect of DC32 on Keap1 degradation could be prevented by p62 knockdown using siRNA. Administration of DC32 could inhibit the activation of Akt/mTOR and ERK, and pretreatment of NIH-3T3 cells with the autophagy inhibitor 3-methyladenine (3-MA) attenuated the degradation of Keap1 induced by DC32. These results suggest that DC32 inhibits the degradation of Nrf2 by promoting p62-mediated selective autophagy and that p62 upregulation contributed to a positive feedback loop for persistent activation of Nrf2. In summary, our present study demonstrated that DC32 significantly suppressed rheumatoid arthritis (RA) via the Nrf2-p62-Keap1 feedback loop by increasing the mRNA and protein levels of Nrf2 and inducing p62 expression. These findings provide new mechanisms for artemisinins in RA treatment and a potential strategy for discovering antirheumatic drugs.

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Section: Introductionmentioning

confidence: 99%

DC32, a Dihydroartemisinin Derivative, Ameliorates Collagen-Induced Arthritis Through an Nrf2-p62-Keap1 Feedback Loop

Fan

Yao

et al. 2018

Front. Immunol.

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“…Many studies demonstrated high sensitivity of FT-IR to analyze the skeletal muscle (Jerônimo et al, 2012), heart muscle (Cheheltani et al 2012), and some biological fluids, such as serum (Carvalho et al, 2014), urine (Khaskheli et al, 2013), and saliva (Khaustova et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

2015

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Introduction: Saliva is the most promising biofluid to monitor the physiological state of athletes, because this method is not invasive and has low contamination risks. The characterization of saliva by Fourier transform infrared spectroscopy (FT-IR) has been studied as an alternative technique to the standard clinical analysis. However, methodological procedures for saliva analysis are not completely clear, especially in terms of influence of storage conditions and sample preparations for infrared analysis. Thawed saliva includes a precipitate, which may influence the infrared spectral analysis. Thus, the purpose of this study was to show the spectral differences of the precipitate, supernatant, and a combo, as well as the best way to classify the physiological state of the athletes by FT-IR. Methods: The saliva collection was performed before, immediately after, and two hours after a handball match. After the storage of samples at -20 ○ C, it was possible to identify two phases (precipitate and supernatant) and to determine the biochemical differences between the spectra of each phase, which were distinctly analyzed by the second derivative and deconvolution bands. Results: The precipitate and supernatant results showed characteristic bands, especially in the protein regions. All FT-IR spectra were also statistically classified by linear discriminant analysis (LDA), using principal component analysis (PCA). The LDA precipitate and supernatant had lower value when compared to combo spectra (Combination of precipitate and supernatant) with 82%, showing that this combination is the best way to discriminate spectra of saliva samples collected before, immediately after, and 2 h after physical effort. Discussion: The results showed that it is possible to differentiate biochemically the two salivary phases, as well as the importance of the homogenization process of saliva samples to classify the physiological status of athletes using FT-IR.

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“…O valor do pH crítico depende da solubilidade dos tecidos duros e da concentração cálcio, fosfato e flúor na solução, pois estes minerais determinarão o grau de saturação da solução e consequentemente direcionarão para a perda ou precipitação mineral, quando a solução se apresentar subsaturada ou supersaturada, em relação ao produto de solubilidade das apatitas dentárias (LUSSI et al, 2011). Soluções ácidas (pH<5,5) com cálcio na sua composição em concentrações maiores que as encontradas no fluido da placa (3,5-8,2mmol CARVALHO, 2014). No presente estudo, a concentração de cálcio variou de 0,289 a 0,501 mmmol/L valores muitos baixos para exercer algum efeito protetor, especialmente nas pastilhas com pH abaixo de 5,5, como observado em alguns produtos analisados neste estudo.…”

Section: Discussionunclassified

O Programa Saúde Na Escola E a Perspectiva De Educação Em Saúde

Mottas¹,

Canal²,

Silva³

et al. 2021

Dinamismo E Clareza No Planejamento Em Ciências Da Saúde 3

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Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético.

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Study of advanced rheumatoid arthritis

Cited by 15 publications

References 73 publications

DC32, a Dihydroartemisinin Derivative, Ameliorates Collagen-Induced Arthritis Through an Nrf2-p62-Keap1 Feedback Loop

DC32, a Dihydroartemisinin Derivative, Ameliorates Collagen-Induced Arthritis Through an Nrf2-p62-Keap1 Feedback Loop

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

O Programa Saúde Na Escola E a Perspectiva De Educação Em Saúde

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