In vitro degradation of poly (L-co-D,L lactic acid) containing PCL-T

Duarte, Marcia Adriana Tomaz; Duek, Eliana Aparecida de Rezende; Motta, Adriana Cristina

doi:10.4322/polimeros.2014.056

Cited by 10 publications

(16 citation statements)

References 15 publications

(16 reference statements)

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“…Furthermore, no significant changes were found in the pH of the incubation solution, throughout all the degradation period, remaining at approximately 7.4 (data not shown). Our study showed a good correlation between the low weight loss of all membranes and insignificant pH variations in the PBS solution, as shown in other studies with similar matrices and time …”

Section: Resultssupporting

confidence: 90%

“…The increase of PLGA incorporation to 30% (w/w) preserved the viability of the cell lines as well as their proliferation capacity in comparison to pure PCL, being similar to pure PLGA. These results are in agreement with previous studies . Conversely, the favorable results presented by the blend PCL70/PLGA30 correlate with high surface roughness presented by this membrane, since the literature has attributed the improvements in cell proliferation to topographic cues promoted by surface roughness …”

Section: Discussionsupporting

confidence: 93%

“…The degradation kinetics is affected by factors related to the physicochemical characteristics of the polymer system. 9,18,23,[37][38][39] In this study, the effects of PCL:PLGA blending and microgrooving were investigated through 90 days of degradation, in an aqueous physiological environment, PBS [ Fig. 3(a,b)].…”

Section: Surface Characterization Young's Modulus and In Vitro Mass mentioning

confidence: 99%

“…Our study showed a good correlation between the low weight loss of all membranes and insignificant pH variations in the PBS solution, as shown in other studies with similar matrices and time. [37][38][39][40] The surface hydrophobicity of the scaffolds is a relevant key factor for governing cell response, which can be assessed by measuring the contact angle through water spread of a droplet on the surface. 26 All smooth membranes indicated favorable hydrophilicity and exhibited water contact angles of 65.8 6 5.68 (pure PCL), 67.5 6 4.18 (PCL/PLGA 90:10), 67.9 6 3.18 (PCL/PLGA 80:20), 65.7 6 1.78 (PCL/ PLGA 70:30) and 76.8 6 1.58 (pure PLGA).…”

Section: Surface Characterization Young's Modulus and In Vitro Mass mentioning

confidence: 99%

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Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Valente

Chagastelles

Nicoletti

et al. 2018

J Biomedical Materials Res

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This study investigated the effects of smooth and microgrooved membrane blends, with different polycaprolactone (PCL)/ poly(lactic-co-glycolic acid) (PLGA) ratios on the viability, proliferation, and adhesion of different mammalian cell types. The polymer matrices with and without microgrooves, obtained by solvent casting, were characterized by field-emission scanning electron microscopy, atomic force microscopy, contact angle and Young's modulus. Blend characterization showed an increase in roughness and stiffness of membranes with 30% PLGA, without any effect on the contact angle value. Pure PCL significantly decreased the viability of Vero, HaCaT, RAW 264.7, and human fetal lung and gingival fibroblast cells, whereas addition of increasing concentrations of PLGA led to a reduced cytotoxicity. Increased proliferation rates were observed for all cell lines. Fibroblasts adhered efficiently to smooth membranes of the PCL70/PLGA30 blend and pure PLGA, compared to pure PCL and silicone. Microgrooved membranes promoted similar cell adhesion for all groups. Microstructured membranes (15 and 20-μm wide grooves) promoted suitable orientation of fibroblasts in both PCL70/PLGA30 and pure PLGA, as compared to pure PCL. Neuronal cells of the dorsal root ganglion exhibited an oriented adhesion to all the tested microgrooved membranes. Data suggest a satisfactory safety profile for the microgrooved PCL70/PLGA30 blend, pointing out this polymer combination as a promising biomaterial for peripheral nerve regeneration when cell orientation is required. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1522-1534, 2018.

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Section: Resultssupporting

confidence: 90%

Section: Discussionsupporting

confidence: 93%

Section: Surface Characterization Young's Modulus and In Vitro Mass mentioning

confidence: 99%

Section: Surface Characterization Young's Modulus and In Vitro Mass mentioning

confidence: 99%

See 2 more Smart Citations

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Valente

Chagastelles

Nicoletti

et al. 2018

J Biomedical Materials Res

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“…O aglomerado desses glóbulos gera uma estrutura porosa. Duarte [17] ao estudar de PLDLA/PCL-T na forma de membranas já havia verificado que a presença do PCL-T modificava a membrana de PLDLA tornando-a porosa.…”

Section: Mev -Microscopia Eletrônica De Varredura (Pinos)unclassified

Caracterização de pinos da blenda poli(L-co-D,L ácido láctico)/poli(caprolactona triol) (PLDLA/PCL-T) e análise das propriedade mecânicas dos pinos durante degradação in vitro

2016

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ResumoOs dispositivos de fixação óssea, metálicos convencionais, usados em cirurgia crâniomaxilofacial têm apresentado alguns problemas, tais como, corrosão, inflamação e infecção, além de neoformação de estrutura óssea mecanicamente inferior devido à atrofia gerada pela diferença de módulo elástico entre metal e osso, razões que têm levado ao aumento do interesse por dispositivos poliméricos bioarreabsorvíveis. Os polímeros biorreabsorvíveis mais utilizados nesta aplicação pertencem à família dos poli (α-hidroxi ácidos), que têm como característica degradarem por hidrólise de suas ligações ésteres, tal como copolímero poli (L-ácido láctico-co-D, L ácido láctico), PLDLA. Neste trabalho foram investigados alguns efeitos da adição de poli (caprolactona triol), PCL-T sobre PLDLA. Foram preparados pinos por fusão de blendas nas seguintes composições 100/0, 90/10, 70/30 and 50/50 (m/m), PLDLA/PCL-T. Os pinos foram caracterizados por diferentes técnicas (DSC, MEV e ensaio mecânico). A degradação in vitro dos pinos foi investigada, sendo observado que a adição de PCL-T no PLDLA modificou suas propriedades mecânicas e morfológicas. Tais mudanças podem apresentar potencial para outras aplicações do material, onde a questão da flexibilidade se faça necessária. Palavras-chaves: PLDLA/PCL-T, caracterização, propriedades mecânicas, in vitro. AbstractThe bone fixation devices, conventional metallic cranium used in surgery have presented some problems, such as corrosion, inflammation and infection, and a lower mechanically newly formed bone structure due to the atrophy caused by the difference in stiffness between metal and bone. These reasons have led to increased interest in bioreabsorbable polymeric devices. The most bioresorbable polymers used in this application belong to the family of poly (α-hydroxy acids), which are characterized degrade by hydrolysis of its ester linkages, such as copolymer poly (L-lactic acid-co-D, L lactic acid) (PLDLA). In this work was investigated some effects of the addition poly (caprolactone triol), PCL-T in the PLDLA. Pins were prepared by melting in the 100/0, 90/10, 70/30 and 50/50 (w/w) compositions, PLDLA/PCL-T. The pins were characterized by different methods (DSC, SEM and mechanical test). The in vitro pins degradation was investigated. It was observed that the addition of PCL-T of PLDLA modifies the mechanical properties, morphological, we conclude that PLDLA/PCL-T can be a potential material for various applications, where the question of flexibility to make necessary.

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Development of co‐electrospun membrane composed poly(l‐co‐d,l‐lactic acid) and natural rubber with potential application in dermal dressing

Pinto,

V. Quevedo,

Komatsu

et al. 2024

J of Applied Polymer Sci

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Wound healing represents a global challenge for the healthcare system, resulting in substantial costs and physical and emotional impacts on individuals. There is an increasing demand for innovative alternatives to treat this condition. The co‐electrospinning technique enables the production of porous membrane composed of natural and synthetic polymers, which exhibit unique functions and adjustable mechanical properties that assist in the tissue healing process. Therefore, the present study aims to investigate the physical–chemical, mechanical, and biological properties of membrane composed of poly(l‐co‐d,l‐lactic acid) (PLDLA) co‐electrospun with different concentrations of natural rubber (NR) (1.5%, 2.0%, and 2.5%). Scanning electron microscopy analysis revealed the porous surface of the membranes. Fourier‐transform infrared spectroscopy confirmed the presence of NR in the material. The low Young's modulus obtained in the mechanical tensile test for the PLDLA membrane co‐electrospun with 1.5% NR indicated its elastic capacity under low tension. The results corroborate with the differential scanning calorimetry, and thermogravimetric analysis, which highlights the compatibility between polymers at this specific proportion, as well as in vitro biocompatibility. The findings underscore the potential of PLDLA and NR co‐electrospun membranes as promising candidates for formulating biomaterials for applications such as dermal dressings.

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In vitro degradation of poly (L-co-D,L lactic acid) containing PCL-T

Cited by 10 publications

References 15 publications

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Caracterização de pinos da blenda poli(L-co-D,L ácido láctico)/poli(caprolactona triol) (PLDLA/PCL-T) e análise das propriedade mecânicas dos pinos durante degradação in vitro

Development of co‐electrospun membrane composed poly(l‐co‐d,l‐lactic acid) and natural rubber with potential application in dermal dressing

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