Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water

Abstract

The chemical and pharmaceutical industries are the main generators of residues, such as phenolic microcontaminants, including catechol, resorcinol, p-nitrophenol and 4-chlorophenol. Therefore, this work aims to identify these microcontaminants through an emerging contaminant biosensor by means of an enzymatic biosensor constructed with an enzymatic extract from the fungus Marasmiellus colocasiae. Based on the differential pulse voltammetry electrochemical technique, the biosensor was used to analyze the patterns of catechol, resorcinol, p-nitrophenol and 4-chlorophenol. The analysis of a sample prepared with these standards in water from the public supply network was also carried out. As a result, it was possible to verify that the biosensor developed in this study is more sensitive than conventional methods and has a greater affinity for catechol. In the sample prepared with the standards, it was possible to qualitatively identify the presence of 4-chlorophenol, resorcinol and catechol. The proposed biosensor was sensitive and has potential for application in the analysis of microcontaminants in the environment with the detection limit = 0.17 µmol L-1, and the quantification limit = 0.52 µmol L-1.

Enzymatic Electroanalytical Biosensor Based on Maramiellus colocasiae Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha

“…Works developed by the research group corroborated the results obtained with the biosensor developed in this research with other immobilized polyphenol oxidases electrodes for the detection of catechol group phenolic compounds, catechins, and galactic acid [ 5 ]. The results presented in Table 3 present compiled biosensors in the literature with the one proposed, comparing the detection limit and electrochemical techniques used.…”

“…Studies have shown that the enzymatic biosensor of M. colocasiae presented relevant results for a new analytical tool with low cost, high sensitivity, and reproducibility, guiding the group’s focus on research with real samples under usual conditions for the detection of phenolic compounds, optimizing the control of quality in green tea, aiming at the development of new electrochemical detection devices [ 5 ].…”

“…Over the years, biosensor technology has drawn attention to the agri-food sector due to the high demand for analyses associated with contamination, adulteration, additives, allergens, and food packing problems in foodstuff [ 1 , 2 ]. In this scenario, electrochemical biosensors are attractive analytical tools that take advantage of remarkable attributes, such as easy-to-operate, sensitive, simple-to-construct, and fast responsive devices for monitoring safety and quality of food [ 3 , 4 , 5 , 6 , 7 ]. Among the components in foodstuffs, phenolic compounds have been highlighted since they provide added value, owing to their well-known health benefits and their function as stabilizers [ 8 ].…”

“…White rot fungi are important phenolic degraders and have intense enzymatic activity, such as the pathogenic fungus Marasmiellus colocasiae [ 18 , 19 , 20 ]. Crude enzymatic extract from Marasmiellus colocasiae is rich in polyphenoloxidase allowing the development of electrochemical biosensors for phenolic compound detection [ 5 ].…”

Development of a sustainable nanosensor using green Cu nanoparticles for simultaneous determination of antibiotics in drinking water

“…Indeed, the cross-use of knowledge in the eld of electrochemistry, nanophysics and nanotechnology of integrated circuits has allowed the design of chemical and biological sensors. The development of electrochemical sensors for the quantication of water contaminants [33][34][35][36][37][38][39][40] and the detection of antibiotics 41,42 has attracted the attention of researchers due to their cost effectiveness, high sensitivity, fast response, and simplicity of sample preparation compared to other methods of analysis. Nonetheless, their practical application in the analysis of real samples has been oen limited by the passivation of the electrode surface.…”