In Vitro Evaluation of Fluoride in Saliva After Topical Application of Professional Use Products

Abstract

Objective: To evaluate in vitro the presence of fluoride in saliva after applying professional use products on the surface of dental enamel. Material and Methods: Experimental groups were composed by: Cariostatic 12% (CA), Fluoridated Varnish 5% (FV), Fluorine Acidulate Gel 1.23% (AG) and Fluorine Neutral Gel 2% (NG). Fluoridated dentifrice (FD) and Artificial Saliva (AS) were used as controls. Products (10 µL) were applied to the surface of bovine enamel blocks (4×4×1 mm, n = 18) and immersed in 10 mL of artificial saliva at room temperature. Aliquots of artificial saliva (750 µL) of each sample were collected 1, 2, 4, 8, 24 and 48 hours after application of the products. Analyses were performed in triplicate, using a fluoride ion-specific electrode coupled to a potentiometer. The equipment was calibrated using a standard curve for fluoride analysis from 0.125 to 64 µgF-/mL. Results: Greater fluoride concentration (µgF-/mL) was observed after 1h application, as follows: 197.40 (NG), 172.21 (AG), 20.25 (CA), 14.49 (FV) e 11.81 (FD). Fluoride concentration increased overtime for all groups. After 48h, the following fluoride concentrations were assessed: 428.12 (AG), 267.25 (NG), 65.36 (FV), and 62.52 (CA). Conclusion: Greater fluoride release was observed for AG and NG groups, mostly after 1h application.

Salivary Fluoride Bioavailability after Brushing with Brazilian Red Propolis Dentifrice: A Clinical Study

“…e TF, TSF, and IF concentrations in saliva significantly increased after brushing with dentifrice in this study, reaching maximum concentrations in the first five minutes of the test, gradually decreasing over time, with no significant difference between groups when comparing the different time points. Similar data have been found in studies such as those by Naumova et al [27], Staun Larsen et al [28], and Bezerra et al [29], showing an increase in concentration in the first samples collected, followed by a gradual decrease inversely proportional to the elapsed time. In addition, the study conducted by Naumova et al [27] presented interindividual variability in fluoride concentrations, similar to those found in the present study.…”

Fluoride release from RMGIC versus Giomer concerning different curing devices at different time intervals (in vitro study)

“…(6) Artificial saliva with no fluoride content was used in the current study to avoid the presence of fluoride ions in natural saliva, which may act as a confounding factor, so that the fluoride amount obtained in this study was the pure amount of fluoride ion derived from RMGIC or Giomer fluoride release. (15,16) Many methods have been employed to estimate the amount of fluoride releases such as spectrophotometry, ion chromatography, fluoride ion-specific electrodes, and capillary electrophoresis. (1) Ion-specific electrode with an ion analyzer was used in this study because it is simple, inexpensive and does not require the use of complex laboratory equipment.…”

“…This pattern of release has been observed in previous studies. (16,20) However, Giomer has no initial burst action as RMGIC but Giomer uses pre-reacted glass ionomer technology to form a stable phase of GIC in the restoration. The more extensive acid-base reaction and hydrogel layer of glass fillers are responsible for the high amount of release in Giomer on the first day.…”

Comparison of the Fluoride Release of Silver Diamine Fluoride, Fluoride Varnish, Acidulated Phosphate Fluoride Gel on Extracted Teeth over Various Time Intervals in Artificial Saliva

“…2018, [32] where fluoride concentration reached its peak immediately after silver diamine fluoride application on enamel and that it had returned to the baseline one hour after application. Bezerra et al 2019 [33] concluded that greater fluoride release was observed for Fluorine Acidulate Gel 1.23% (AG) and Fluorine Neutral Gel 2% (NG) groups after 1hr application compared to Cariostatic 12% (CA), Fluoridated Varnish 5% (FV).…”