Production of Medical Grade Silicone for Facial Prosthesis with Bactericidal Properties from the Inclusion of Poly (Diallyldimethylammonium Chloride): An in Vitro Study

Santos, Rennan Luiz Oliveira dos; Gamarra, Juan Gonzalo Aliaga; Lincopán, Nilton; Petri, Denise Freitas Siqueira; Paula, Claudete Rodrigues; Coto, Neide Pena; Dias, Reinaldo Brito e

doi:10.4034/pboci.2019.191.15

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…PDADMAC is often applied in biotechnology for dendronized polymer (DP) gelator formation [ 65 ] and water treatment [ 46 ]. It is deposited on a solid substrate serving as an “anchor layer” [ 66 ] used for producing multilayers (films) of various coverage and structure [ 67 ], in anion-exchange membranes for fuel cells [ 68 ], and in the design of dental materials [ 69 ].…”

Section: Macroionsmentioning

confidence: 99%

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

2023

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Growth factors are a class of proteins that play a role in the proliferation (the increase in the number of cells resulting from cell division) and differentiation (when a cell undergoes changes in gene expression becoming a more specific type of cell) of cells. They can have both positive (accelerating the normal healing process) and negative effects (causing cancer) on disease progression and have potential applications in gene therapy and wound healing. However, their short half-life, low stability, and susceptibility to degradation by enzymes at body temperature make them easily degradable in vivo. To improve their effectiveness and stability, growth factors require carriers for delivery that protect them from heat, pH changes, and proteolysis. These carriers should also be able to deliver the growth factors to their intended destination. This review focuses on the current scientific literature concerning the physicochemical properties (such as biocompatibility, high affinity for binding growth factors, improved bioactivity and stability of the growth factors, protection from heat, pH changes or appropriate electric charge for growth factor attachment via electrostatic interactions) of macroions, growth factors, and macroion-growth factor assemblies, as well as their potential uses in medicine (e.g., diabetic wound healing, tissue regeneration, and cancer therapy). Specific attention is given to three types of growth factors: vascular endothelial growth factors, human fibroblast growth factors, and neurotrophins, as well as selected biocompatible synthetic macroions (obtained through standard polymerization techniques) and polysaccharides (natural macroions composed of repeating monomeric units of monosaccharides). Understanding the mechanisms by which growth factors bind to potential carriers could lead to more effective delivery methods for these proteins, which are of significant interest in the diagnosis and treatment of neurodegenerative and civilization diseases, as well as in the healing of chronic wounds.

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Section: Macroionsmentioning

confidence: 99%

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

2023

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“…In the literature, the antibacterial effect of silicon is well known and documented. However, some studies show no antibacterial activity or even a growth accelerating effect of silicon on Staphylococcus aureus [ 41 , 42 ]. Thus, a slight increase in the number of S. aureus cells surviving the contact with the Si/Au-enriched sample observed in comparison with HT and Au-HT materials may be a consequence of both combined phenomena: bactericidal effect of Au and growth stimulating effect of Si.…”

Section: Resultsmentioning

confidence: 99%

Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

et al. 2021

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Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

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“…The cellular viability and proliferation of L929 cells were assessed after 3 and 5 d of culture using MTT assay kit. The prepared LbL coated samples were exposed to gamma radiation (with a dose of 25 KGy) for sterilization, because sterilization using ultraviolet light has a disruptive effect on PDADMAC structure [49,50]. Each sterile sample with a surface area of 6 cm 2 was added with 1 ml of culture medium.…”

Section: Mtt Assaymentioning

confidence: 99%

Electrospun zein nanofibers loaded with curcumin as a wound dressing: enhancing properties with PSS and PDADMAC layers

Salehi,

Ghaee,

Moris

et al. 2024

Biomed. Mater.

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Development of wound dressings with enhanced therapeutic properties is of great interest in the modern healthcare. In this study, a zein-based nanofibrous wound dressing containing curcumin as a therapeutic agent was fabricated through electrospinning technique. In order to achieve desirable properties, such as antibacterial characteristics, reduced contact angle, and enhanced mechanical properties, the layer-by-layer technique (LbL) was used for coating the surfaces of drug-loaded nanofibers by sequentially incorporating poly (sodium 4-styrene sulfonate) (PSS) as a polyanion and poly (diallyldimethylammonium chloride) (PDADMAC) as a polycation. Various analyses, including scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, drug release assessment., and mechanical tests were employed to assess the characteristics of the prepared wound dressings. Based on the results, coating with polyelectrolytes enhanced the Young's modulus and tensile strength of the electrospun mat from 1.34 MPa and 4.21 MPa to 1.88 MPa and 8.83 MPa, respectively. The coating also improved the controlled release of curcumin and antioxidant activity, while the outer layer, PDADMAC, exhibited antibacterial properties. The cell viability tests proved the appropriate biocompatibility of the prepared wound dressings. Moreover, our findings show that incorporation of the coating layers enhances cell migration and provides a favorable surface for cell attachment. According to the findings of this study, the fabricated nanofibrous wound dressing can be considered a promising and effective therapeutic intervention for wound management, facilitating the healing process.

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Production of Medical Grade Silicone for Facial Prosthesis with Bactericidal Properties from the Inclusion of Poly (Diallyldimethylammonium Chloride): An in Vitro Study

Cited by 6 publications

References 19 publications

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Electrospun zein nanofibers loaded with curcumin as a wound dressing: enhancing properties with PSS and PDADMAC layers

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