<b>Short-term storage of lebranche mullet <i>Mugil liza</i> (Valenciennes, 1836) semen <i>in natura</i> and diluted with CF-HBSS

Abstract

The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis -SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane integrity was maintained in fresh semen up to 6h, and it was 15-20% higher than in natura semen from 6 to 48h (p < 0.05). The use of CF-HBSS as diluent in the ratio 1:3 is recommended for semen refrigeration of lebranche mullet.

Characterization of short‐term storage of the semen ofTrachelyopterus galeatus(Siluriformes: Auchenipteridae) incorporating dimethylsulphoxide (dmso) to the diluent

“…Trachelyopterus galeatus sperm remain continuously mobile after collection. And the incorporation of other substances into the thinner, such as antioxidants, amino acids, organic acids, cryoprotectants and others, can improve storage time and the performance of sperm during cold storage (Magnotti, de Castro, et al, 2018).…”

Effect of ascorbic acid supplementation on zootechnical performance, haematological parameters and sperm quality of lebranche mullet Mugil liza

“…Sperm motility was activated with oceanic water at salinity of 36, pH 8.55 and temperature of 20.7°C (Magnotti, Castro, et al, ; Magnotti, Figueroa, et al, ). Immediately after activation, a single observer performed optical microscopy (ICC50 HD, Leica Microsystems; 200×) in random fields of each slide without light.…”

“…Livers and gonads were sampled (n = 3 per tank, n = 9 per treatment) to determine gonadosomatic index (GSI) and hepatosomatic index (HSI) (Vazzoler, 1982) Fish were anaesthetized with benzocaine (50 mg/L) in the urogenital region and the anal fins were cleaned and dried with paper towels. Using light abdominal pressure, semen was collected in 1 ml syringes (0.02 ml scale) and immediately stored in ice (without direct contact with the syringes) at 4 ± 2°C (air temperature) protected from light (Magnotti, Castro, et al, 2018;Magnotti, Figueroa, et al, 2018). Samples contaminated with faeces or urine were discarded (Cosson et al, 2008).…”

“…Sperm characteristics vary among fish species and are also influenced by season, reproductive period, environment, climate and the method of cell collection (Solis-Murgas, Felizardo, Ferreira, Andrade, & Veras, 2011). Some semen characteristics of mullet were described by Magnotti, Figueroa, et al (2018) and Magnotti, Castro, et al (2018), analysing cultured (F1)-also in first maturation, and wild species during reproductive migration. The cultured mullets presented better results for cell membrane integrity, motility rate and spermatic density (96 ± 13%, 83.6 ± 15.7% and 31.8 ± 2.9 × 10 9 ml −1 respectively).…”

ADVANCES IN REPRODUCTION OF THE LEBRANCHE MULLET <i>Mugil liza</i>: MATURATION AND SPAWNING OF F1 BREEDERS IN CAPTIVITY

“…The reproductive period began when females presented average oocyte diameter greater than 600 µm, value considered suitable for hormonal induction (Cerqueira et al, 2017). Males were mature and able to reproduce when they released semen after abdominal massage (Magnotti et al, 2018a(Magnotti et al, , 2018bCastro et al, 2019).…”