Expression of genes related to soil flooding tolerance in soybeans

Casarotto, Gabriele; Kaspary, Tiago Edu; Cutti, Luan; Thomas, Andre Luis; Neto, José Fernandes Barbosa

doi:10.4025/actasciagron.v41i1.42709

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…In addition, the expression of some potential candidate genes for flooding tolerance might be genotype-specific. Similar results with higher expression of a few potential candidate genes under flooding stress were observed even in susceptible genotype (Casarotto et al 2019). So, it can be considered that these two genes, Glyma.10g050300 and Glyma.12g030900, are potential candidate genes for flooding tolerance in soybean.…”

Section: Qrt-pcr and Gene Expressionsupporting

confidence: 81%

Integrating RNA Sequencing and Quantitative Trait Locus Mapping to Identify Potential Candidate Genes for Flooding Tolerance in Soybean

Dhungana

Seo

Park

et al. 2021

Korean J. Breed. Sci.

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Flooding stress causes a significant reduction in soybean yield. The development of flood-tolerant cultivars is an effective way to minimize yield loss due to flooding. Information on candidate genes for flooding tolerance is useful for developing tolerant lines. The objective of this study was to identify potential candidate genes for flooding tolerance in soybean by integrating the results of a quantitative trait locus analysis and RNA sequencing. A total of 19 genes showed good amplification in capillary electrophoresis and were further analyzed through a reverse transcription quantitative polymerase chain reaction (qRT-PCR); two of these genes showed differential expression among tolerant and susceptible lines. The expression of Glyma.12g030900 and Glyma.10g050300 in leaf and root tissues, respectively, was higher in several tolerant lines than in the susceptible lines under flooding stress. The chlorophyll index of the tolerant lines was also consistently higher than that of the susceptible lines over two years, supporting the qRT-PCR results. This study provides useful information on flooding tolerance in soybeans.

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Section: Qrt-pcr and Gene Expressionsupporting

confidence: 81%

Integrating RNA Sequencing and Quantitative Trait Locus Mapping to Identify Potential Candidate Genes for Flooding Tolerance in Soybean

Dhungana

Seo

Park

et al. 2021

Korean J. Breed. Sci.

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“…As NO induces GmGLB1, GmGLB1 would be induced by abiotic stresses that increase NO. Valliyodan et al (2014) and Casarotto et al (2019) have already reported that flooding induces expression of GmGLB1 and it may contribute to flooding tolerance. As NO was produced and the expression of GmGLB1 was strongly induced by flooding but not by salt (Figures 2, 6A).…”

Section: Discussionmentioning

confidence: 96%

Expression of soybean plant hemoglobin gene family under abiotic stresses

Araragi

Ikeura

Uchiumi

2021

Plant Biotechnology

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Many abiotic stresses induce the generation of nitric oxide (NO) in plant tissues, where it functions as a signal molecule in stress responses. Plants modulate NO by oxidizing it to NO 3 − with plant hemoglobin (GLB), because excess NO is toxic to cells. At least eight genes encoding GLB have been identified in soybean, in three clades: GLB1, GLB2, and GLB3. However, it is still unclear which GLB genes are responsible for NO regulation under abiotic stress in soybean. We exposed soybean roots to flooding, salt, and two NO donors-sodium pentacyanonitrosylferrate (III) dihydrate (SNP) and S-nitroso-N-acetyl-d,l-penicillamine (SNAP)-and analyzed expression of GLB genes. GmGLB1, one of two GLB1 genes of soybean, significantly responded to both SNP and SNAP, and its induction was almost completely repressed by a NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. GmGLB1 responded to flooding but not to salt, suggesting that it is responsible for NO regulation under NO-inducing abiotic stresses such as flooding. GmGLB3, one of two GLB3 genes of soybean, did not respond to NO donors at all but did respond to flooding, at a lower level than GmGLB1. These results suggest that flooding induces not only NO but also unknown factor(s) that induce GmGLB3 gene in soybean.

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“…On the other hand, in Medicago sativa , many differentially expressed genes related to photosynthesis and nitrogen-metabolism-related genes were identified after waterlogging stress [ 140 ]. Transcriptome analysis in G. max after waterlogging stress showed a set of differentially expressed genes related to ET signaling, energy metabolism, and fermentation pathways [ 141 ]. During waterlogging stress in wheat plants, a transcriptome analysis was performed which showed a number of differentially expressed genes associated with oxidoreductase activity and biological response to ABA and SA [ 40 ].…”

Section: Strategies For Improving Waterlogging Tolerance In Plants: P...mentioning

confidence: 99%

Exploring the Potential of Multiomics and Other Integrative Approaches for Improving Waterlogging Tolerance in Plants

Tyagi

Ali

Park

et al. 2023

Plants

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Soil flooding has emerged as a serious threat to modern agriculture due to the rapid global warming and climate change, resulting in catastrophic crop damage and yield losses. The most detrimental effects of waterlogging in plants are hypoxia, decreased nutrient uptake, photosynthesis inhibition, energy crisis, and microbiome alterations, all of which result in plant death. Although significant advancement has been made in mitigating waterlogging stress, it remains largely enigmatic how plants perceive flood signals and translate them for their adaptive responses at a molecular level. With the advent of multiomics, there has been significant progress in understanding and decoding the intricacy of how plants respond to different stressors which have paved the way towards the development of climate-resistant smart crops. In this review, we have provided the overview of the effect of waterlogging in plants, signaling (calcium, reactive oxygen species, nitric oxide, hormones), and adaptive responses. Secondly, we discussed an insight into past, present, and future prospects of waterlogging tolerance focusing on conventional breeding, transgenic, multiomics, and gene-editing approaches. In addition, we have also highlighted the importance of panomics for developing waterlogging-tolerant cultivars. Furthermore, we have discussed the role of high-throughput phenotyping in the screening of complex waterlogging-tolerant traits. Finally, we addressed the current challenges and future perspectives of waterlogging signal perception and transduction in plants, which warrants future investigation.

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Expression of genes related to soil flooding tolerance in soybeans

Cited by 6 publications

References 30 publications

Integrating RNA Sequencing and Quantitative Trait Locus Mapping to Identify Potential Candidate Genes for Flooding Tolerance in Soybean

Integrating RNA Sequencing and Quantitative Trait Locus Mapping to Identify Potential Candidate Genes for Flooding Tolerance in Soybean

Expression of soybean plant hemoglobin gene family under abiotic stresses

Exploring the Potential of Multiomics and Other Integrative Approaches for Improving Waterlogging Tolerance in Plants

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