Improvement of the specific detection of &lt;i&gt;Xanthomonas phaseoli&lt;/i&gt; pv. &lt;i&gt;manihotis&lt;/i&gt; based on the &lt;i&gt;pthB&lt;/i&gt; gene

Melo, Rita de Cássia Cerqueira; Bragança, C. A. D.; Pestana, Kátia Nogueira; Silva, Harllen Sandro Alves da; Ferreira, Cláudia Fortes; Oliveira, S. A. S. de

doi:10.4025/actasciagron.v41i1.42708

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Three PCR-based methods were reported for Xpm detection recently. The first method used an optimized version of a previous assay (Verdier et al, 1998) resulting in improved Xpm detection (Cerqueira-Melo et al, 2019). The second approach was based on a multiplexed nested PCR including a broadly conserved fragment of Xpm transcription activator-like (TAL) effector genes and a semispecific region of rpoB, resulting in a wider detection potential for Xpm strains (Bernal-Galeano et al, 2018).…”

Section: Diagnostic Toolsmentioning

confidence: 99%

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Zárate-Chaves¹,

Cruz

López

et al. 2021

Molecular Plant Pathology

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Cassava is an important staple crop as a source of food and income for hundreds of millions of people in tropical countries. This major crop is threatened by several pests and pathogens that significantly affect productivity. Among bacterial pathogens, the vascular and systemic Xanthomonas phaseoli pv. manihotis (Xpm) has received considerable attention due to its devastating potential in the tropics and scientific importance worldwide (Mansfield et al., 2012). This pathogen profile describes the main hallmarks of this pathosystem and updates several reviews and original articles on the subject.Although more bacterial pathogens infect cassava, information in the literature is scarce. In this regard, we also present a compilation of information for a second xanthomonad infecting cassava, the nonvascular pathogen X. cassavae. | D IS E A S E DE SCRIP TI ON AND EPIDEMI OLOGY | Cassava bacterial blight symptomsCassava bacterial blight (CBB), which is caused by the bacterial pathogen Xpm, is characterized by a range of symptoms that

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Section: Diagnostic Toolsmentioning

confidence: 99%

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Zárate-Chaves¹,

Cruz

López

et al. 2021

Molecular Plant Pathology

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Genetic diversity of Xanthomonas phaseoli p v. manihotis populations using rep-PCR and VNTR molecular markers

Oliveira,

Moreira

et al. 2023

Pesq. agropec. bras.

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The objective of this work was to evaluate the genetic diversity of Xanthomonas phaseoli p v. manihotis (Xpm) from eight populations from five cassava producing states in Brazil, through the rep-PCR (BOX-PCR and ERIC-PCR) and variable number of tandem repeat (VNTR) markers. Cassava leaves with symptoms of cassava bacterial blight were collected in eight municipalities, and the Xpm isolates were identified by amplification with primers specific for these isolates. The identity of the Xpm isolates was confirmed with the BOX-PCR, ERIC-PCR, and VNTR markers. The observed selection pressure, together with the mode of reproduction and the mechanisms that increase genetic variability, allows of the pathogen populations to adapt according to microclimate variation, contributing to a differentiated reproductive success. ERIC-PCR and VNTRs are the best markers for evaluating the genetic variability in the eight studied Xpm populations. However, ERIC-PCR is the marker that best separated the groups by population and presented a higher similarity between the isolates of the same population. The study of the genetic diversity of Xpm is key to improve disease monitoring and management strategies in cassava crops.

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Phylogenomic Analysis Supports the Transfer of 20 Pathovars from Xanthomonas campestris into Xanthomonas euvesicatoria

et al. 2023

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The Gram-negative bacterial genus Xanthomonas includes numerous infra-specific taxa known as pathovars, which are defined primarily on host range and disease symptoms. With the advent of molecular sequence data, many pathovars have been transferred from X. campestris into other Xanthomonas species to better harmonise taxonomy and phylogeny. We performed whole-genome shotgun sequencing on pathotype strains of the following X. campestris pathovars: blepharidis, carissae, clerodendri, convolvuli, coriandri, daturae, euphorbiae, fici, heliotropii, ionidii, lawsoniae, mirabilis, obscurae, paulliniae, pennamericanum, spermacoces, uppalii, vernoniae, viegasii and zingibericola. These genomes showed more than 98% average nucleotide identity with the type-strain of X. euvesicatoria and less than 88% with the type-strain of X. campestris. We propose the transfer of these pathovars into X. euvesicatoria and present an emended species description for X. euvesicatoria.

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Improvement of the specific detection of <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> based on the <i>pthB</i> gene

Cited by 3 publications

References 20 publications

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Cassava diseases caused byXanthomonas phaseolipv.manihotisandXanthomonas cassavae

Genetic diversity of Xanthomonas phaseoli p v. manihotis populations using rep-PCR and VNTR molecular markers

Phylogenomic Analysis Supports the Transfer of 20 Pathovars from Xanthomonas campestris into Xanthomonas euvesicatoria

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