“…Therefore, the type, size, age, position and orientation of explant used in plant tissue culture and in the multiplication stage (Stage II) of micropropagation are all of great importance Raad et al, 2012b;Pinheiro et al, 2013;Cardoso and Habermann, 2014;Pinheiro et al, 2014). Explants also have different demands for in vitro conditions, like PGRs, nutrient composition of the medium, temperature or light (Gahan and George, 2008;Pinheiro et al, 2013;Cardoso and Habermann, 2014).…”