Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã

“…The formation of three types of calli also identified in the banana plant. These authors observed that the Type I calli with transparent and aqueous appearance had elongated cells, Type II was characterized by small yellow aggregations containing clustered cells with greater embryogenic potential, and Type III with large yellow clusters with isodiametric cells 26 . In oil palm trees, the presence of diverse types of calli in the same culture medium was also observed, and by means of cytochemical analyses, the authors identified that the beige and globular calli have embryogenic characteristics 19 .…”

Section: Resultsmentioning

confidence: 99%

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

Batista

Mendonça

Pádua

et al. 2018

Braz. arch. biol. technol.

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The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the growth regulator dicamba (1.13, 4.52, and 9.04 µM) and without it. At 60 days of culturing, the calli were analyzed by scanning electron microscopy and at 90 days were evaluated by light microscopy in regard to the embryogenic characteristics of the cells. Different type of calli were induced in leaf explants, designated as Type I with light yellow coloring, Type II with dark yellow coloring, and Type III of brown coloring; however, only Type I had embryogenic characteristics. In the meristematic explants, only one type of callus was induced, and it had embryogenic characteristics. At 90 days of culturing, the formation of somatic embryos in the different embryogenic stages was observed and the formation of procambium, protoderm, and ground meristem tissues. At 150 days of culturing, the concentration of 1.13 µM of dicamba was prominent in the formation of somatic embryos in the different embryogenic stages.

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“…3B). According to Ribeiro et al (2012) such cells are seen in non-embryogenic callus. Cells with such morphology were also observed by other researchers in the callus of different plants.…”

Section: Morphological and Ultra-structural Analysismentioning

confidence: 99%

Remedial effect of ascorbic acid and citric acid on oxidative browning of Glycyrrhiza glabra callus cultures

Vijayalakshmi¹,

Shourie²

2016

bta

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Oxidative browning is a common and severe problem in plant tissue cultures including callus cultures of Glycyrrhiza glabra , where browning is caused by accumulation and oxidation of phenolic compounds. Brown callus results in decreased biosynthetic potential and poor growth, eventually leading to cell death. In this study, the effect of different concentrations and combinations of antioxidants-ascorbic acid, and citric acid on browning of G.glabra callus was studied. Morphological and ultra-structural changes related to browning and antioxidant treatment were also studied using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to evaluate the cellular characteristics and metabolic activity of control and treated callus. Intensity of browning in callus remarkably decreased with increasing concentrations of ascorbic acid; the browning was completely overcome at a concentration of 20 mg/l ascorbic acid, in combination with 10 mg/l citric acid. An electron microscopy analysis showed that brown callus was found to have ruptured cell surface and a disorganized cell structure with the absence of cell organelles that affected callus development and lead to cell death. Non-brown callus had all the characteristics typical for a non-embryogenic callus cells with high-level activity showing all vital cell organelles including abundant and elongated mitochondria. Therefore, we conclude that ascorbic acid and citric acid have wide applications in tissue cultures of Glycyrrhiza glabra where browning restricts the callus development, and lead to the establishment of non-embryogenic callus with high metabolic activity for further use in secondary metabolite production.

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Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã

Cited by 9 publications

References 17 publications

Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars

Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis

Remedial effect of ascorbic acid and citric acid on oxidative browning of Glycyrrhiza glabra callus cultures

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