Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’

Prizão, Eliane Cristina; Gonçalves, Letícia de Menezes; Milaneze-Gutierre, Maria Auxiliadora; Mangolin, Claudete Aparecida; Machado, Maria de Fátima Pires da Silva

doi:10.4025/actasciagron.v34i2.12257

Cited by 13 publications

(9 citation statements)

References 10 publications

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“…reported that apart from darkening the medium, activated charcoal is related to significant results concerning root growth of the plantlets (PRIZÃO et al, 2012). Dezan et al (2012), for example, found that the reduction in the salt concentration of MS culture medium was beneficial for the in vitro development of Schomburgkia gloriosa plantlets, as similarly found in this study with the same species.…”

Section: Resultssupporting

confidence: 80%

Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

Hunhoff

Lage

Palú³

et al. 2018

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Tissue culture is an alternative form of producing healthy, vigorous and regular plants on a large scale. The purpose of this study was to evaluate the most efficient culture medium for in vitro plantlet germination and development of three Orchidaceae species. Seeds disinfested of three species were dispersed in distilled water and dripped into basic Murashige and Skoog (MS) medium. The experimental design was completely randomized in a factorial 3 x 4 (three species x four culture media), with 5 replications. Four treatments were established: (1) full-strength MS medium, with the full nutrient concentration (MSØ), (2) full-strength MS medium plus 0.3% activated charcoal (MSØ ACh), (3) half-strength MS medium (½ MS) and (4) half-strength MS medium with 0.3 % activated charcoal (½ MS ACh). Germination was evaluated after 15, 20, 25, 30, and 60 days. The shoot height, leaf number and length, root number and length of plantlets of the three studied species were assessed. In A. variegata, 73% germinated after 60 days in ½ MS ACh medium. In the same period, 100% of E. viparum and S. gloriosa seeds germinated in MSØ ACh medium. The plant height, leaf number and length, root number and length were significantly higher for the species A. variegata and E. viviparum in MSØ ACh medium. The culture media ½ MS and MSØ with addition of activated charcoal favored in vitro germination for the three orchid species of this study. Keywords: culture medium, orchids, tissue culture Aspasia variegata, Epidendrum viviparum, Schomburgkia gloriosa. RESUMO Requerimentos nutricionais para germinação e desenvolvimento in vitro de três espéciesde Orchidaceae do sudoeste da Amazônia brasileira A cultura de tecidos é aplicada como alternativa de produção de mudas sadias, vigorosas, uniformes e em larga escala. O objetivo deste estudo foi avaliar o meio de cultura mais eficiente para a germinação e desenvolvimento in vitro de plântulas de três espécies de Orchidaceae. Sementes desinfestadas das três espécies foram colocadas em água destilada e gotejadas em meio de cultura básico de Murashige and Skoog (MS). O desenho experimental foi completamente ao acaso em um fatorial 3 x 4 (três espécies x quatro meios de cultura) com cinco repetições. Constituído de quatro tratamentos, sendo: (1) meio MS com concentração total de nutrientes (MSØ), (2) meio MS com concentração total de nutrientes acrescido de 0,3% de carvão ativado (MSØ C.A), (3) meio MS com metade das concentrações de nutrientes (½ MS) e (4) meio MS com metade das concentrações de nutrientes, acrescido de 0,3 % de carvão ativado (½ MS C.A). A avaliação da germinação foi realizada aos 15, 20, 25, 30 e 60 dias. Foram mensuradas altura da parte aérea, número e comprimento das folhas e número e comprimento de raízes de plântulas das três espécies estudadas. Em A. variegata verificou-se uma germinação de 73% aos 60 dias em meio ½ MS C.A. E. viviparum e S. gloriosa obtiveram 100 % de germinação para o mesmo período, em meio MSØ C.A. A altura da planta, número e comprimento de folhas, n...

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Section: Resultssupporting

confidence: 80%

Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

Hunhoff

Lage

Palú³

et al. 2018

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“…Species of the Cattleya genus and their hybrids are currently the most commercialized orchids in Brazil due to their large, colorful flowers (PRIZÃO et al, 2012). The asymbiotic cultivation of orchids can be used to reduce the effects of their predation in forests, to obtain in vitro germination of a large fraction of their seeds, and to make possible the re-establishment of these species in natural environments.…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

et al. 2016

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ABSTRACT:Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L -1 of sucrose, solidified with 6 g. L -1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0 C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L -1 ). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L -1 ) promoted higher dry mass accumulation and better control of water loss by plants.

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“…Therefore, in general activated charcoal presence was benefi cial for the development of the aerial part of the seedlings. PRIZÃO et al (2012) confi rmed that the presence of charcoal was particularly important in inducing roots and sprouts in the double hybrid of orchid 'BLC Pastoral Innocence' when inoculated in medium without growth regulator.…”

Section: Resultsmentioning

confidence: 89%

“…This shows that seedlings cultivated in the medium with higher salt availability and charcoal presence benefi ted in terms of growth parameters. PRIZÃO et al (2012) confi rmed that the use of activated charcoal is useful for root induction in seedlings of Cattleya bicolor.…”

Section: Resultsmentioning

confidence: 93%

In vitro behaviour of Aspasia variegata, an epiphytic orchid from the Brazilian Cerrado

et al. 2013

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Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’

Cited by 13 publications

References 10 publications

Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids

In vitro behaviour of Aspasia variegata, an epiphytic orchid from the Brazilian Cerrado

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