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Decontaminating leaf samples from crops sprayed with pesticides and nutrient solutions is important for foliar analysis. This study evaluated the effect of different washing methods in coffee and sorghum foliage that had been sprayed with zinc (with or without surfactant). The plants were sprayed with a 3 g L-1 zinc sulfate solution, with and without surfactant. Seven days later, leaves were collected and washed. The experiment was completely randomized in a 2 x 2 x 3 + 2 factorial, with three replications. The first factor represents foliar zinc applications with or without surfactant, the second represents the number of washes (1 or 2) and the third represents the concentration of the wash solution (detergent + hydrochloric acid) at (0 + 0 mL L-1; 1.0 + 3.5 mL L-1 and 2.0 + 7.0 mL L-1). The last one represents two additional treatments without washing (zinc sprayed with and without surfactant). Surfactant strengthens contact between zinc and foliage and enhances absorption. Washing is an indispensable pretreatment for leaf analysis and our study showed that a single wash with detergent + hydrochloric acid (1.0+ 3.5 mL L-1) was the most effective washing method for coffee and sorghum.
Decontaminating leaf samples from crops sprayed with pesticides and nutrient solutions is important for foliar analysis. This study evaluated the effect of different washing methods in coffee and sorghum foliage that had been sprayed with zinc (with or without surfactant). The plants were sprayed with a 3 g L-1 zinc sulfate solution, with and without surfactant. Seven days later, leaves were collected and washed. The experiment was completely randomized in a 2 x 2 x 3 + 2 factorial, with three replications. The first factor represents foliar zinc applications with or without surfactant, the second represents the number of washes (1 or 2) and the third represents the concentration of the wash solution (detergent + hydrochloric acid) at (0 + 0 mL L-1; 1.0 + 3.5 mL L-1 and 2.0 + 7.0 mL L-1). The last one represents two additional treatments without washing (zinc sprayed with and without surfactant). Surfactant strengthens contact between zinc and foliage and enhances absorption. Washing is an indispensable pretreatment for leaf analysis and our study showed that a single wash with detergent + hydrochloric acid (1.0+ 3.5 mL L-1) was the most effective washing method for coffee and sorghum.
This article empirically provides a scientific production trends overview of coffee agronomy at the global level, allowing us to understand the structure of the epistemic community on this topic. The knowledge contributions documented are examined using a bibliometric approach (spatial, productive, and relational) based on data from 1618 records stored in the Web of Science (JCR and ESCI) between 1963 and May 2021, applying traditional bibliometric laws and using VOSviewer for the massive treatment of data and metadata. At the results level, there was an exponential increase in scientific production in the last six decades, with a concentration on only 15 specific journals; the insertion of new investigative peripheral and semiperipheral countries and organizations in worldwide relevance coauthorship networks, an evolution of almost 60 years in relevant thematic issues; and a co-occurring concentration in three large blocks: environmental sustainability of forestry, biological growth variables of coffee, and biotechnology of coffee species; topic blocks that, although in interaction, constitute three specific communities of knowledge production that have been delineated over time.
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