Musa paradisiaca L. var Semeru (MPS) is a banana with thick skin that produces unused waste. The potential anxiolytic effect of banana peel ethanol extract (BPE) is explored in this study. There were seven groups in this study, namely the normal control group, CMC Na, alprazolam (0.4 mg/kg), tryptophan (270 mg/kg), 5-HTP (18 mg/kg), BPE (140, 280 mg/kg). The number of rats per group in the light-dark box (LDA) test was 4. Meanwhile, the number of rats per group in the elevated plus maze (EPM) test was 6. BPE (140 and 280 mg/kg) was given to rats one hour before the anxiolytic test using a LDA and EPM. BPE (140 and 280 mg/kg) did not significantly increase entries and time spent in the light chamber of the light-dark box. In addition, it also did not significantly affect entries and time spent in open arms in the elevated plus maze. Referring to the LDA and EPM tests, the ethanol extract of MPS did not significantly reduce anxiety.