The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay

Gonçalves, Macks Wendhell; Gambale, Priscilla Guedes; Godoy, Fernanda Ribeiro; Alves, Alessandro Arruda; Rezende, Pedro H. de Almeida; Cruz, Aparecido Divino da; Maciel, Natan Medeiros; Nomura, Fausto; Marco, Paulo De; Silva, Daniela de Melo e

doi:10.3897/zoologia.34.e19865

Cited by 21 publications

(6 citation statements)

References 39 publications

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“…The conducted studies mainly focused on the evaluation of environmental stressors such as agrochemicals and heavy metals in vivo. [223,[227][228][229][230][231][232][233][234][235][236][237], fungicides (captan) [238], pesticides [239,240], antiparasitic agents [239], persistent organic pollutants (POPs) [219] as well as other agrochemicals [241] and poultry litter [242]. These studies indicated the DNA damaging effects of selected chemicals as well as using amphibians as sentinel organisms for the evaluation of environmental pollution.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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“…Of the three species considered in the present study, Gonçalves et al (2019) concluded that D. minutus is the most sensitive to agricultural environments in terms of DNA damage, followed by B. albopunctata and, lastly, P. cuvieri . In fact, P. cuvieri is generally considered to be a good bioindicator for the detection of the genotoxic effects of agricultural insecticides in the field (Gonçalves et al, 2017b), which is why this species was selected for the histopathological analyses of the liver presented in our study.…”

Section: Discussionmentioning

confidence: 99%

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

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Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

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“…Amphibians are excellent bioindicators of environmental health and hence, a continued decline in their populations indicates the deterioration of environmental quality (DeGarady & Halbrook, 2006; Gonçalves et al, 2017; Hodkinson & Jackson, 2005; Linder & Grillitsch, 2000). Moreover, they are integral components of many ecosystems, acting as prey, predators, or herbivores at different trophic levels of the food chain.…”

Section: Introductionmentioning

confidence: 99%

Does atrazine induce changes in predator recognition, growth, morphology, and metamorphic traits of larval skipper frogs (Euphlyctis cyanophlyctis)?

Supekar¹,

Gramapurohit

2022

J Exp Zool Pt A

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Atrazine, an info disruptor, interferes with the olfaction of aquatic organisms by impairing the chemosensory system. Consequently, it affects behavior, physiology, and growth increases mortality and infections, and suppresses the immune system of aquatic animals. In this study, we wanted to determine the sensitivity of larval Euphlyctis cyanophlyctis to different concentrations of atrazine by assessing their antipredator behavior, growth, morphology, and metamorphic traits. The results indicate that exposure to atrazine did not affect the survival of tadpoles. However, it caused retarded growth at higher concentrations. Interestingly, the antipredator behavior of tadpoles toward conspecific alarm cues decreased in a dose‐dependent manner with an increase in the concentration of atrazine. Tadpoles exposed to low concentrations of atrazine had deeper, wider bodies and tails while those exposed to higher concentrations had shallower and narrower bodies with shallower tail muscles. However, at low and moderate concentrations atrazine did not affect size at metamorphosis, it extended the larval duration at higher concentrations.

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The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay

Cited by 21 publications

References 39 publications

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Does atrazine induce changes in predator recognition, growth, morphology, and metamorphic traits of larval skipper frogs (Euphlyctis cyanophlyctis)?

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