Identification of novel single nucleotide polymorphisms in the growth hormone ( GH ) gene in Anatolian water buffalo ( Bubalus bubalis ) populations in Turkey

Ünal, Emel Özkan; Işık, Raziye; Soysal, M. I.

doi:10.37496/rbz4920190260

Revista Brasileira De Zootecnia

2020

DOI: 10.37496/rbz4920190260

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Identification of novel single nucleotide polymorphisms in the growth hormone ( GH ) gene in Anatolian water buffalo ( Bubalus bubalis ) populations in Turkey

Emel Özkan Ünal

Raziye Işık

M. I. Soysal

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2023

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Cited by 2 publications

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Cloning, Sequencing and Expression of Goonch Bagarius bagarius (Hamilton, 1822) Growth Hormone

Muhammad,

Bashir

2023

Croatian Journal of Fisheries

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Goonch Bagarius bagarius (Hamilton, 1822) is one of the largest and fastest-growing catfish of the Indus River found at Taunsa Barrage, Pakistan. The full-length cDNA of the growth hormone gene (600 bp) was amplified by reverse transcription of mRNA isolated from the pituitary gland of B. bagarius. The full-length growth hormone gene encodes a putative polypeptide of 200 amino acids with a molecular mass of 22.58 kDa. The precursor of B. bagarius growth hormone (GH) is composed of 22 amino acids as a signal peptide and 178 amino acids of a mature peptide. There were six conserved Cys residues in GH protein (20, 71, 135, 173, 190 and 198) that maintain the structural integrity of this protein. One putative N-glycosylation site was present at the 197th amino acid. The total number of positively charged (Arg and Lys) and negatively charged (Asp and Glu) residues is 42 and 31, respectively. The B. bagarius GH gene shows more than 90% sequence homology with other catfishes. The mature protein GH gene was expressed in Escherichia coli using pET-28 expression vector, and the recombinant protein of 19.5 kDa was detected through SDS-PAGE analysis. This study suggests that cloning and expression of B. bagarius GH gene would provide basic information for transgenic studies aimed at a faster growth rate. This recombinant GH may be produced on a large scale to exploit its growth-promoting function in other cultured fishes.

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Cloning, Sequencing and Expression of Goonch Bagarius bagarius (Hamilton, 1822) Growth Hormone

Muhammad,

Bashir

2023

Croatian Journal of Fisheries

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Polymorphisms of Growth Hormone and Calpastatin Genes and Their Association with Sheep Meat Quality

Surov,

Skorykh,

Skokova

et al. 2023

Russ. Agricult. Sci.

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Identification of novel single nucleotide polymorphisms in the growth hormone ( GH ) gene in Anatolian water buffalo ( Bubalus bubalis ) populations in Turkey

Cited by 2 publications

References 38 publications

Cloning, Sequencing and Expression of Goonch Bagarius bagarius (Hamilton, 1822) Growth Hormone

Cloning, Sequencing and Expression of Goonch Bagarius bagarius (Hamilton, 1822) Growth Hormone

Polymorphisms of Growth Hormone and Calpastatin Genes and Their Association with Sheep Meat Quality

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