Single-Walled Carbon Nanotubes as Enhancing Substrates for PNA-Based Amperometric Genosensors

Fortunati, Simone; Rozzi, Andrea; Curti, Federica; Giannetto, Marco; Corradini, Roberto; Careri, Maria

doi:10.3390/s19030588

Cited by 16 publications

(7 citation statements)

References 26 publications

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“…Here, a layer by layer form of polypyrrole and SWCNT provided a useful solid matrix for enzyme immobilization, indicating a good performance with excellent linearity from 1 mM to 50 mM of glucose concentration, and high sensitivity of 7.06 uA/mM. In addition, an electrochemical sensor implemented on SWCNT combining screen printed electrodes [13] and glassy carbon electrode (GCE) [31] were also introduced for better performances.…”

Section: Single-wall Carbon Nanotubesmentioning

confidence: 99%

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

2020

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Background: The electrochemical biosensor is one of the typical sensing devices based on transducing the biochemical events to electrical signals. In this type of sensor, an electrode is a key component that is employed as a solid support for immobilization of biomolecules and electron movement. Thanks to numerous nanomaterials that possess the large surface area, synergic effects are enabled by improving loading capacity and the mass transport of reactants for achieving high performance in terms of analytical sensitivity. Main body: We categorized the current electrochemical biosensors into two groups, carbon-based (carbon nanotubes and graphene) and non-carbon-based nanomaterials (metallic and silica nanoparticles, nanowire, and indium tin oxide, organic materials). The carbon allotropes can be employed as an electrode and supporting scaffolds due to their large active surface area as well as an effective electron transfer rate. We also discussed the non-carbon nanomaterials that are used as alternative supporting components of the electrode for improving the electrochemical properties of biosensors. Conclusion: Although several functional nanomaterials have provided the innovative solid substrate for high performances, developing on-site version of biosensor that meets enough sensitivity along with high reproducibility still remains a challenge. In particular, the matrix interference from real samples which seriously affects the biomolecular interaction still remains the most critical issues that need to be solved for practical aspect in the electrochemical biosensor. Recently, various electrochemistry-driven biosensing methods have been introduced for simple and miniaturized analytical devices for on-site analysis. This trend can be applied to replace the commercial lab instruments manufactured by the renowned in vitro diagnosis

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Section: Single-wall Carbon Nanotubesmentioning

confidence: 99%

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

2020

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“…A broad spectrum of compounds that find applications in healthcare diagnosis and POC analysis of diseases can be detected with such biosensor materials [22]. Carbon allotrope-based nanomaterials consisting of fullerenes, [23,24,25,26,27] nanotubes (CNT) [28,29,30,31,32,33], films of graphene and its derivatives [34,35,36,37], quantum dots [38,39,40,41], and nanodiamonds [42,43,44,45,46,47] play a substantial role in recent advancements in the biosensor domain. In addition to greater sensitivity and novel mechanisms, such sensors offer a higher spatial resolution in case of localised detection along with real-time and label-free non-destructive sensing.…”

Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

Nanomaterials for Healthcare Biosensing Applications

Pirzada

Altıntaş

2019

Sensors

153

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In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field. Nanomaterials introduce versatility to the sensing platforms and may even allow mobility between different detection mechanisms. The prospect of a combination of different nanomaterials allows an exploitation of their synergistic additive and novel properties for sensor development. This paper covers more than 290 research works since 2015, elaborating the diverse roles played by various nanomaterials in the biosensing field. Hence, we provide a comprehensive review of the healthcare sensing applications of nanomaterials, covering carbon allotrope-based, inorganic, and organic nanomaterials. These sensing systems are able to detect a wide variety of clinically relevant molecules, like nucleic acids, viruses, bacteria, cancer antigens, pharmaceuticals and narcotic drugs, toxins, contaminants, as well as entire cells in various sensing media, ranging from buffers to more complex environments such as urine, blood or sputum. Thus, the latest advancements reviewed in this paper hold tremendous potential for the application of nanomaterials in the early screening of diseases and point-of-care testing.

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“…To overcome these limitations, the scientific community has focused on the engineering and development of innovative biosensors, which offer remarkable versatility in terms of target species that can be quantified. In fact, by applying a suitable biomolecule to be used as a receptor, it is possible to target mainly antibodies [ 17 , 18 , 19 , 20 ], antigens [ 21 , 22 , 23 , 24 ] or oligonucleotides [ 25 , 26 , 27 , 28 ]. Furthermore, biosensing methods are usually rapid, implemented in portable and cost-effective analytical devices as well as in smart PoCT devices for analytical signal acquisition.…”

Section: Introductionmentioning

confidence: 99%

Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor

et al. 2022

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An IoT-WiFi smart and portable electrochemical immunosensor for the quantification of SARS-CoV-2 spike protein was developed with integrated machine learning features. The immunoenzymatic sensor is based on the immobilization of monoclonal antibodies directed at the SARS-CoV-2 S1 subunit on Screen-Printed Electrodes functionalized with gold nanoparticles. The analytical protocol involves a single-step sample incubation. Immunosensor performance was validated in a viral transfer medium which is commonly used for the desorption of nasopharyngeal swabs. Remarkable specificity of the response was demonstrated by testing H1N1 Hemagglutinin from swine-origin influenza A virus and Spike Protein S1 from Middle East respiratory syndrome coronavirus. Machine learning was successfully used for data processing and analysis. Different support vector machine classifiers were evaluated, proving that algorithms affect the classifier accuracy. The test accuracy of the best classification model in terms of true positive/true negative sample classification was 97.3%. In addition, the ML algorithm can be easily integrated into cloud-based portable Wi-Fi devices. Finally, the immunosensor was successfully tested using a third generation replicating incompetent lentiviral vector pseudotyped with SARS-CoV-2 spike glycoprotein, thus proving the applicability of the immunosensor to whole virus detection.

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Single-Walled Carbon Nanotubes as Enhancing Substrates for PNA-Based Amperometric Genosensors

Cited by 16 publications

References 26 publications

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

Nanomaterials for Healthcare Biosensing Applications

Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor

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