Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

Hernandes, Camila; Gueuvoghlanian-Silva, Bárbara Yasmin; Monnaka, Vitor Ulisses; Ribeiro, Natalia Mazini; Pereira, Welbert Oliveira; Podgaec, Sérgio

doi:10.31744/einstein_journal/2020ao5294

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Our group investigated the expression of toll-like receptors (TLRs), in T reg cells isolated from peritoneal fluid from patients with and without endometriosis, and we found that there is a change in the activation of TLRs receptors in women with endometriosis, where TLR 1, 2, 3, 4, 5, 6, 7 and 8 were expressed in regulatory T cells isolated from peritoneal fluid from women with endometriosis while only TLR1 and TLR2 receptors were expressed in women without endometriosis [41].…”

Section: Discussionmentioning

confidence: 86%

Microbiome Profile of Deep Endometriosis Patients: Comparison of Vaginal Fluid, Endometrium and Lesion

Hernandes

Silveira²,

Sereia³

et al. 2020

Diagnostics

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This work aimed to identify and compare the bacterial patterns present in endometriotic lesions, eutopic endometrium and vaginal fluid from endometriosis patients with those found in the vaginal fluid and eutopic endometrium of control patients. Vaginal fluid, eutopic endometrium and endometriotic lesions were collected. DNA was extracted and the samples were analyzed to identify microbiome by high-throughput DNA sequencing of the 16S rRNA marker gene. Amplicon sequencing from vaginal fluid, eutopic endometrium and endometriotic lesion resulted in similar profiles of microorganisms, composed most abundantly by the genus Lactobacillus, Gardnerella, Streptococcus and Prevotella. No significant differences were found in the diversity analysis of microbiome profiles between control and endometriotic patients; however deep endometriotic lesions seems to present different bacterial composition, less predominant of Lactobacillus and with more abundant Alishewanella, Enterococcus and Pseudomonas.

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Section: Discussionmentioning

confidence: 86%

Microbiome Profile of Deep Endometriosis Patients: Comparison of Vaginal Fluid, Endometrium and Lesion

Hernandes

Silveira²,

Sereia³

et al. 2020

Diagnostics

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“…The expression of innate immune system-related Toll-like receptors 2 and 4 was found in the ovarian cumulus cells and granulosa cells during ovulation [ 53 ]. It is found that other Toll-like receptor types are found in regulatory T cells from patients with endometriosis [ 54 ]. MAPK1 is involved in regulating cell proliferation and apoptosis [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Targets and Associated Signaling Pathways of Jingshu Granules in Ovarian Cysts Based on Systemic Pharmacological Analysis

Zhu

et al. 2021

BioMed Research International

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Background. More than a third of women could develop ovarian cysts during their lifetime. Jingshu granules are used for the treatment of gynecological disease of primary dysmenorrhea. However, the molecular mechanisms of Jingshu granules in ovarian cysts are still unreported. We aimed to find the active ingredients, molecular targets, and potential signaling pathways of Jingshu granules in ovarian cysts by using the systemic pharmacological analysis. Methods. Firstly, the effect of Jingshu granules on female hormones and reproductive organs of young female rats was evaluated. Secondly, candidate pharmaceutical ingredients of Jingshu granules were retrieved from the traditional Chinese medicine systems pharmacology (TCMSP) database and analysis platform. Potential protein targets for the active ingredients in Jingshu granules were then identified according to the oral bioavailability and drug-likeness indices. Thirdly, ovarian cyst-related gene targets were screened based on different databases. Finally, enrichment analysis was used to analyze the potential biological function of intersection targets between Jingshu granules and ovarian cysts. Results. In young female rats, Jingshu granules reduced the secretion of estradiol, progesterone, and prolactin and could affect the development of the uterus. This suggested that Jingshu granules played roles in hormone secretion and reproduction. From the TCMSP, a total of 1021 pharmaceutical ingredients of Jingshu granules were retrieved. After further screening, a total of 166 active ingredients and 159 protein targets of Jingshu granules were identified. In addition, 4488 gene targets of ovarian cysts were screened out. After taking the intersection, a total of 110 intersection targets were identified between potential protein targets of Jingshu granules and gene targets of ovarian cysts. In the functional analysis of 110 intersection targets, 8 signaling pathways including progesterone-mediated oocyte maturation (MAPK8 and CDK1 involved), GnRH signaling pathway (JUN involved), T cell receptor signaling pathway and Toll-like receptor signaling pathway (MAPK1 involved), NOD-like receptor signaling pathway (TNF, IL6, and IL1B involved), p53 signaling pathway (CDK2 and CDK4 involved), VEGF signaling pathway (MAPK14 involved), and PPAR signaling pathway (PPARG involved) were obtained. Conclusion. Our study revealed that Jingshu granules could function in patients with ovarian cysts through a number of molecular targets and signaling pathways. Our study may provide a new field into the mechanisms of Jingshu granules in ovarian cysts, from the molecular to the signaling pathway level.

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“…Another factor is the mechanisms that modulate the inflammatory response through Toll-Like receptors (TLR) located on the membrane of regulatory T lymphocytes. TLRs, present in greater quantity and variety in women with endometriosis, can identify microorganisms in retrograde menstrual blood and respond with exacerbation of the inflammatory response and consequent worsening of pain [10].…”

Section: Endometriosis and Painmentioning

confidence: 99%

Approach to Pain in Endometriosis Patients: Systematic Literature Review

Costa¹,

Gonçalves²,

Leite³

et al. 2022

JBM

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Background: Endometriosis is a gynecological, multifactorial condition that affects women's life quality, especially due to the pain inherent to it. Pain is caused by physical and psychological aspects; therefore, several factors must be analyzed, aiming to treat the whole. Conventional, drug or surgical treatments are the most used; however, the search for alternative therapeutic methods is growing. Objective: To analyze the approach to pain in women with endometriosis, seeking a better understanding of the pathophysiological mechanisms of this disease, its impact on life quality, as well as the means of treatments in use and innovations in this field. Methods: Systematic literature review, conducted in the Virtual Health Library databases between 2010 and 2021, restricted to articles in English and Portuguese with the descriptors: Endometriosis, Pain, and Treatment. After reading the abstracts, 197 articles were found, and, after reading them, 59 articles were selected. Results: This review analyzed 59 scientific studies that rigorously met the previously established characteristics in the sample selection. The synthesis included the following aspects: author/year of publication, article title, objective, type of study and database. Related factors such as acute or chronic pain, late diagnosis, discrediting symptomatic complaints and trivialization by health professionals have a profound impact on the physical and mental health of women with endometriosis. On the other hand, there are several classes of drugs, surgical procedures, and alternative therapies available for pain management. Conclusion: Given the context addressed, a certain need is suggested for health professionals to better understand the disease and adopt new behaviors or improve existing ones, such as reception, active listening, early diagnosis and comprehensive and individualized treatment. In addition, further studies are needed regarding alternative therapies, seeking to expand the scientific evidence of their benefits.

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Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

Cited by 7 publications

References 25 publications

Microbiome Profile of Deep Endometriosis Patients: Comparison of Vaginal Fluid, Endometrium and Lesion

Microbiome Profile of Deep Endometriosis Patients: Comparison of Vaginal Fluid, Endometrium and Lesion

Molecular Targets and Associated Signaling Pathways of Jingshu Granules in Ovarian Cysts Based on Systemic Pharmacological Analysis

Approach to Pain in Endometriosis Patients: Systematic Literature Review

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