Inflammatory biomarkers in sera of patients with intervertebral disc degeneration

Rodrigues, Luciano Miller Reis; Oliveira, Lilian Zerbinatti de; Silva, Mariane de Barros Ribeiro da; Accardo, Camila de Melo; Giglio, Adriana Braz del; Pinhal, Maria Aparecida da Silva

doi:10.31744/einstein_journal/2019ao4637

Cited by 12 publications

(7 citation statements)

References 29 publications

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“…Several studies have highlighted the important roles of proinflammatory mediators and inflammation responses during IVDD. 23 , 38 Upon degeneration, NP and AF become inflamed, and this inflammatory milieu is associated with a cascade of degenerative events that may eventually result in discogenic pain in patients. 23 , 38 However, the landscape of immune cells and their relationship in the development of IVDD have not been completely understood.…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptomics reveals heterogeneity and intercellular crosstalk in human intervertebral disc degeneration

Wang¹,

Li²,

Huang³

et al. 2023

iScience

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Section: Discussionmentioning

confidence: 99%

Single-cell transcriptomics reveals heterogeneity and intercellular crosstalk in human intervertebral disc degeneration

Wang¹,

Li²,

Huang³

et al. 2023

iScience

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“…According to the literature, IL-1β significantly increased the expression of IL-6 and IL-8, VEGF, chemotactic factor (MCP-1) and disk degradation factor (MMP-3) in IVD cells during hypoxia [21]. The level of IL-6 was elevated both in the patients’ serum with DDD and spinal injuries, but the immunohistochemical expression of IL-6 was significantly higher in the degenerative discs tissue [22].…”

Section: Discussionmentioning

confidence: 99%

Immunomorphogenesis in degenerative disc disease: the role of proinflammatory cytokines and angiogenesis factors

Pravdyuk

Novikova

et al. 2023

Preprint

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Back pain (BP) due to degenerative spinal injury is a severe, often disabling disease and it tends to decrease age of onset. The cellular and molecular basis of the disc and adjacent structures degradation remains in the focus of pathophysiologists and pathomorphologists close attention. The aim of the study was to determine the expression level of proinflammatory cytokines (IL-1β, IL-6, IL-17) and angiogenesis markers (VEGF-A and CD31) in intervertebral disc (IVD) tissue, their association between each other and between disc degeneration in young people with discogenic BP. In patients who underwent discectomy for a disc herniation, a clinical examination, magnetic resonance imaging (MRI) of the lumbar spine, histological and immunohistochemical analyses of these factors in the disc tissue were performed in comparison with the parameters of healthy group samples (controls). Histology image analysis of IVD fragments of the degenerative disc disease (DDD) group detected zones of inflammatory infiltration, combined with vascularization, the presence of granulation tissue and clusters of chondrocytes in the tissue of nucleus pulposus (NP). Statistically significant expression of IL-1β, IL-6, IL-17 and VEGF-A (especially on the chondrocytes surface) and CD31 (in the disc matrix) was evident in the samples of the DDD group compared with the controls (p < 0.0001), that showed a strong correlation with the histological disc degeneration stage (r > 0.5; p < 0.0001). This denotes a high immunoinflammatory potential of chondrocytes and demonstrates their altered morphogenetic properties. The coincidence of the spatial expression of IL-1β and IL-17 in the perivascular zone endothelium and in the vascular lumen (p < 0.01) was found, which point at the inflammatory synergy of these cytokines. High expression of VEGF-A prevailed on the surface of chondrocytes in cell clusters compared to the matrix (p < 0.0001), indicating that the NP cells trigger the angiogenesis. The absence of CD31 in the cracks of NP with high expression in the disc matrix states the secondary nature of IVD defects due to degeneration, and not due to vascular ingrowth. A high number of patients with Modic changes according to MRI data shown the contribution of cytokines to the formation of reactive spondylitis and the clinical course of BP. These results obtained will help to development molecular/cellular targets and basic strategies for therapy of BP with DDD in the early stages in young people.

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“…В тоже время IgA к антигену CD74 не исследовались у пациентов с ДДЗП и хронической болью в спине. В качестве биомаркеров воспаления у пациентов с ДДЗП изучался уровень интерлейкина 6, активность катепсина В, гиалуроновой кислоты в сыворотке крови [14,15]. По результатам этих исследований было установлено, что пациенты с ДДЗП имеют незначительное повышение уровня интерлейкинов.…”

Section: Discussionunclassified

The Level of IgA Antibodies to CD74 in Patients with Spondyloarthritis and Degenerative-Dystrophic Diseases of the Spine

Ребров

Gaydukova

Апаркина

et al. 2022

Arhivʺ vnutrennej mediciny

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Background. According to the scientific literature, anti-CD74 IgA antibodies (IgA anti-CD74) are considered as a possible marker for the diagnosis of axial spondyloarthritis (SpA). The level of IgA anti-CD74 in patients with back pain due to degenerative spine disease has not been studied. Therefore, it could be interesting to compare the serum levels of IgA anti-CD74 in patients with chronic back pain in various diseases. Aim: to compare the levels of IgA anti-CD74 in patients with SpA and degenerative spine diseases. Material and methods. A total of 87 SpA patients (55 male, mean age 41 [29; 49] years) fulfilling the Assessment of Spondyloarthritis International Society (2009) criteria for Axial SpA, and 39 patients (25 male, mean age 45 [34; 53] years) with neurologist-verified degenerative spine diseases (ICD 10 codes — M 51.1 and M 54.4) were enrolled to the study. The serum levels of IgA anti-CD74 were analyzed by enzyme-linked immunosorbent assay (ELISA) in all patients. Results. The median levels of IgA anti-CD74 in patients with SpA were 11.3 [5.4; 19.4] U/ml, in patients with degenerative spine disease — 6.9 [4.5; 13.7] U/ml (p=0.024). IgA anti-CD74 serum levels were above the cut-off value in 58 (66.7 %) patients with SpA and only in 11 (28.2 %) patients with degenerative spine disease (p<0,001). The elevated serum levels of IgA anti-CD74 were detected in 10 (40 %) of 25 male patients and in 1 (7.1 %) of 14 female patients (p = 0.029, χ2 = 4.785) with degenerative spine disease. Conclusion. Serum levels of I gA anti-CD74 were increased in two-thirds of patients with SpA. IgA anti-CD74 was significantly higher in SpA patients compared to patients with degenerative spine disease. >< 0,001). The elevated serum levels of IgA anti-CD74 were detected in 10 (40 %) of 25 male patients and in 1 (7.1 %) of 14 female patients (p = 0.029, χ2 = 4.785) with degenerative spine disease. Conclusion. Serum levels of I gA anti-CD74 were increased in two-thirds of patients with SpA. IgA anti-CD74 was significantly higher in SpA patients compared to patients with degenerative spine disease.

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Inflammatory biomarkers in sera of patients with intervertebral disc degeneration

Cited by 12 publications

References 29 publications

Single-cell transcriptomics reveals heterogeneity and intercellular crosstalk in human intervertebral disc degeneration

Single-cell transcriptomics reveals heterogeneity and intercellular crosstalk in human intervertebral disc degeneration

Immunomorphogenesis in degenerative disc disease: the role of proinflammatory cytokines and angiogenesis factors

The Level of IgA Antibodies to CD74 in Patients with Spondyloarthritis and Degenerative-Dystrophic Diseases of the Spine

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