Characterization of rotavirus strains from day care centers: pre- and post-rotavirus vaccine era

Morillo, Simone Guadagnucci; Luchs, Adriana; Cilli, Audrey; Costa, Fernanda Faria; Carmona, Rita de Cássia Compagnoli; Timenetsky, María do Carmo Sampaio Tavares

doi:10.2223/jped.1981

Cited by 24 publications

(29 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Morillo et al demonstraram que o genótipo G2P [4] foi a única cepa observada em 2007 durante uma vigilância de genótipos de RV em crianças < 5 anos com gastroenterite aguda provenientes de creches no estado de São Paulo por um período de 5 anos 9 . Entretanto, investigações mais detalhadas sobre a proteção heterológica conferida pela vacina monovalente contra o RV são pontos centrais para entender seu comportamento imunológico 7,10 .…”

Section: Introductionunclassified

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

et al. 2011

Self Cite

View full text Add to dashboard Cite

Objective: To monitor rotavirus (RV) and norovirus (NoV) infections in hospitalized children ≤ 5 years with acute gastroenteritis in the state of São Paulo, Brazil, during a 6-year period (2004)(2005)(2006)(2007)(2008)(2009).Methods: This retrospective study was conducted with 61 medical centers with convenient surveillance fecal specimens, investigated by enzyme-linked immunosorbent assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis, reverse polymerase chain reaction and sequencing to genotype characterization.Results: RV and NoV infections were detected in 29.6% (144∕487) and 29.2% (26/89) of the samples, respectively. The most frequent RV genotypes detected were G9P [8] Métodos: Este estudo retrospectivo foi realizado em 61 centros médicos, utilizando amostras fecais coletadas por conveniência, analisadas por ensaio imunoenzimático, eletroforese em gel de poliacrilamida, transcrição reversa seguida de reação em cadeia pela polimerase e sequenciamento para caracterização dos genótipos. Conclusões:A distribuição dos genótipos de RV variou com os anos, acompanhada pela redução no número de casos detectados. Ė necessário intensificar a vigilância pós-implantação da vacina contra RV, visando monitorar as cepas circulantes e sua eficácia contra possíveis genótipos emergentes. Os NoVs têm sido cada vez mais identificados como agentes etiológicos relevantes entre crianças hospitalizadas e exercem um papel importante na etiologia viral da gastroenterite pediátrica aguda no estado de São Paulo. Artigo submetido em 07.04.11, aceito em 15.06.11. J Pediatr (Rio J)

show abstract

Section: Introductionunclassified

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…1 RVA is classified into different P and G genotypes based upon the main neutralization antigens, namely, the spike protein (VP4) and the major outer capsid glycoprotein (VP7). The most prevalent genotypes, G1P [8], G2P [4], G3P [8], G4P [8], and G9P [8], are responsible for approximately 90% of worldwide human RVA infections. 2 However, RVA epidemiology is constantly changing, mainly due to reassortment events, and interspecies transmission of animal RVA to humans.…”

Section: Introductionmentioning

confidence: 99%

“…Rotarix TM is a monovalent vaccine derived from a human G1P [8] strain, and has been introduced in Brazilian Immunization Program since 2006. 4,6 Rotateq TM is a human-bovine reassortant RVA vaccine that contains the human genotypes G1, G2, G3, G4, and P [8], 6 and is available at private clinics. The post-marketing surveillance of circulating RVA genotypes is crucial for vaccine efficacy studies.…”

Section: Introductionmentioning

confidence: 99%

Amino Acid sequence analysis of the two major outer Capsid Proteins (VP7 and VP4) from human-derived canine G3P[3] Rotavirus Strain Detected in Brazil

Luchs¹,

Timenetsky

2013

J Health Biol Sci.

Self Cite

View full text Add to dashboard Cite

Introduction:A close look at the rotavirus group A (RVA) genotypes in Brazil revealed the detection of a rare G3P [3] strain close related to canine strains. The aim of this study was to add to the already known genetic analysis by the description of the G3P[3] (IAL-R2638 strain) amino acid characteristics. Methods: Amino acid sequence analysis and protein based trees were conducted using BioEdit and MEGA 4.0. Results: The VP7 and VP4 protein of the IAL-R2638 strain displayed the highest amino acid identity to the canine-derived human strain HCR3A (99.2%), and to the canine strain RV52/96 (96.4%), respectively. IAL-R2638 strain did not possess an extra VP7 N-linked glycosylation site at amino acid 238 recently described for some G3 strains, as well as RotaTeq TM G3 vaccine strain. The topology exhibited by phylogenetic trees in previous analysis were maintained in the present amino acid-based trees, reinforcing a stable relationship between G3P[3] strains. Conclusions: Amino acid analysis data were consistent with the previous sequence of data obtained for the IAL-R2638 strain, supporting its possible canine origin. Theoretically, RotaTeq TM vaccine could efficiently protect against G3P[3] infections based on the lack of the extra VP7 N-linked glycosylation site at amino acid 238. Phylogenetic analysis hypothesizes that all features undergo evolution independently of each other; however, unfavorable effects of nucleotide substitutions may be compensated by substitutions in other positions. The present study raises the question as to whether the amino acid-based trees could be applied as an approach to the study of RVA evolution, avoiding incorrect phylogenetic reconstructions. ResumoIntrodução: A vigilância epidemiológica dos rotavírus do grupo A (RVA) no Brasil possibilitou a detecção de cepa rara G3P[3] geneticamente semelhante às cepas caninas. O objetivo do presente estudo foi realizar a análise de aminoácidos da cepa G3P[3] (IAL-R2638) e complementar as informações adquiridas por meio da análise nucleotídica. Métodos: A análise da sequência de aminoácidos e a construção das árvores proteicas foi realizada utilizando os programas BioEdit e MEGA 4.0. Resultados: As proteínas VP7 e VP4 da cepa IAL-R2638 exibiram maior identidade de aminoácidos com a cepa humana derivada de cão HCR3A (99,2%) e com a cepa canina RV52/96 (96,4%), respectivamente. A cepa IAL-R2638 não apresenta o sítio adicional de glicosilação N na posição 238 na proteína VP7, recentemente descrito em algumas cepas G3, assim como a cepa G3 vacinal da vacina RotaTeq TM . As topologias exibidas nas árvores filogenéticas previamente analisadas foram mantidas nas árvores baseadas em aminoácidos, reforçando o parentesco estável entre cepas G3P [3]. Conclusões: A análise proteica da cepa IAL-R2638 foi consistente com os dados genéticos obitidos previamente, corroborando sua possível origem canina. Teoricamente, a vacina RotaTeq TM pode ser eficaz contra infecções por G3P[3], uma vez que essas cepas não possuem o sítio extra de glicosilação N 238. Anális...

show abstract

“…1,2 Rotavirus (RV) is the most important etiological agent of diarrhea in children under five years old, and accounts for 111 million episodes of gastroenteritis in the world, and for approximately 611,000 deaths annually, mainly in developing countries. 2 In Brazil, several studies were conducted to demonstrate the importance of RV in children morbidity and mortality.…”

Section: Introductionmentioning

confidence: 99%

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Pereira¹,

Raboni²,

Nogueira³

et al. 2011

Braz J Infect Dis

View full text Add to dashboard Cite

Background and objectives: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea. Methods: 293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE). Results: Rotavirus was detected in 34.8% of samples by LA assay, 28.3% of samples by EIA assay and in 25.6% of samples by PAGE assay. Considering the PAGE method as gold standard, the sensitivity, specificity and accuracy of EIA were 94.6%, 94.4% and 94.5%, and to LA were 82.6%, 81.6% and 81.9%, respectively. Conclusions: These results indicate that antigen detection by EIA is a rapid, sensitive and specific method, and could be used in large-scale applications for screening stool samples suspected of RV infection. This study showed decreased incidence of RV infection in hospitalized children prior to the implementation of the national immunization program against RV.

show abstract

Characterization of rotavirus strains from day care centers: pre- and post-rotavirus vaccine era

Cited by 24 publications

References 15 publications

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

Amino Acid sequence analysis of the two major outer Capsid Proteins (VP7 and VP4) from human-derived canine G3P[3] Rotavirus Strain Detected in Brazil

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Contact Info

Product

Resources

About